Reference on EC 4.6.1.24 - ribonuclease T1
Please use the Reference Search for a specific query.
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Uchida, T.; Egami, F.
Microbial ribonucleases with special reference to RNases T1, T2, N1, and U2
The Enzymes, 3rd Ed. (Boyer, P. D. , ed. )
4
205-250
1971
Aspergillus oryzae
-
Takahashi, K.; Moore, S.
Ribonuclease T1
The Enzymes, 3rd Ed. (Boyer, P. D. , ed. )
15
435-468
1982
Aspergillus oryzae
-
Pace, C.N.; Grimsley, G.R.; Barnett, B.J.
Purification of ribonuclease T1
Anal. Biochem.
167
418-422
1987
Aspergillus oryzae
Jervis, L.; Pettit, N.M.
Purification of ribonuclease T1 on porous glass affinity adsorbents
J. Chromatogr.
97
33-38
1974
Aspergillus oryzae
Fields, R.; Dixon, H.B.F.; Law, G.R.; Yui, C.
Purification of ribonuclease T 1 by diethylaminoethylcellulose chromatography
Biochem. J.
121
591-596
1971
Aspergillus oryzae
Kanaya, S.; Uchida, T.
Purification of ribonuclease T1 by affinity chromatography
J. Biochem.
89
591-597
1981
Aspergillus oryzae
Oobatake, M.; Takahashi, S.; Ooi, T.
Conformational stability of ribonuclease T1. I. Thermal denaturation and effects of salts
J. Biochem.
86
55-63
1979
Aspergillus oryzae
Oobatake, M.; Takahashi, S.; Ooi, T.
Conformational stability of ribonuclease T1. II. Salt-induced renaturation
J. Biochem.
86
65-70
1979
Aspergillus oryzae
Tamaoki, H.; Sakiyama, F.; Narita, K.
Chemical modification of ribonuclease T1 with ozone
J. Biochem.
83
771-781
1978
Aspergillus oryzae
Ruterjans, H.; Pongs, O.
On the mechanism of action of ribonuclease T1. Nuclear magnetic resonance study on the active site
Eur. J. Biochem.
18
313-318
1971
Aspergillus oryzae
Takahashi, K.
The structure and function of ribonuclease T1. VII. Further investigations on amino acid composition and some other properties of ribonuclease T1
J. Biochem.
60
239-245
1966
Aspergillus oryzae
Ito, H.; Hagiwara, M.; Takahashi, K.; Ichikizaki, I.
The structure and function of ribonuclease T1 XXIV. Preparation and properties of a stable water-insoluble polyacrylamide derivative of ribonuclease T1
J. Biochem.
82
877-883
1977
Aspergillus oryzae
Koepke, J.; Maslowska, M.; Heinemann, U.; Saenger, W.
Three-dimensional structure of ribonuclease T1 complexed with guanylyl-2,5-guanosine at 1.8 A resolution
J. Mol. Biol.
206
475-488
1989
Aspergillus oryzae
Pace, C.N.; Grimsley, G.R.
Ribonuclease T1 is stabilized by cation and anion binding
Biochemistry
27
3242-3246
1988
Aspergillus oryzae
Walz, F.G.; Kitareewan, S.
Spermine stabilization of folded ribonuclease T1
J. Biol. Chem.
265
7127-7137
1990
Aspergillus oryzae
Thomson, J.A.; Shirley, B.A.; Grimsley, G.R.; Pace, C.N.
Conformational stability and mechanism of folding of ribonuclease T1
J. Biol. Chem.
264
11614-11620
1989
Aspergillus oryzae
Kiefhaber, T.; Quaas, R.; Hahn, U.; Schmid, F.X.
Folding of ribonuclease T1. 1. Existence of multiple unfolded states created by proline isomerization
Biochemistry
29
3053-3061
1990
Aspergillus oryzae
Pace, C.N.; Laurents, D.V.; Thomson, J.A.
pH Dependence of the urea and guanidine hydrochloride denaturation of ribonuclease A and ribonuclease T1
Biochemistry
29
2564-2572
1990
Aspergillus oryzae
Lee, J.C.
Preparation and properties of water-insoluble derivatives of ribonuclease T1
Biochim. Biophys. Acta
235
435-441
1971
Aspergillus oryzae
Kasai, K.; Uchida, T.; Egami, F.; Yoshida, K.; Nomoto, M.
Purification and crystallization of ribonuclease N1 from Neurospora crassa
J. Biochem.
66
389-396
1969
Neurospora crassa
Lindberg, R.A.; Drucker, H.
Characterization and comparison of a Neurospora crassa RNase purified from cultures undergoing each of three different states of derepression
J. Bacteriol.
157
375-379
1984
Neurospora crassa
Kenney, W.C.; Dekker, C.A.
Ribonuclease U1. Physical and chemical characterization of the purified enzyme
Biochemistry
10
4962-4970
1971
Ustilago sphaerogena
Hashimoto, J.; Uchida, T.; Egami, F.
Purification of ribonuclease U 1 and some properties of ribonucleases U 1 and N 1
J. Biochem.
70
903-911
1971
Ustilago sphaerogena
Fletcher, P.L.; Hash, J.H.
Ribonuclease of Chalaropsis species. I. Isolation and physical properties
Biochemistry
11
4274-4280
1972
Chalara sp.
Fletcher, P.L.; Hash, J.H.
Ribonuclease of Chalaropsis species. II. Chemical properties
Biochemistry
11
4281-4285
1972
Chalara sp.
Nomachi, Y.; Komano, T.
Purification and some properties of two acid ribonucleases from the mycelia of Aspergillus niger
J. Gen. Appl. Microbiol.
26
375-385
1980
Aspergillus niger
-
Sevcik, J.; Sanishvili, R.G.; Pavlovsky, A.G.; Polyakov, K.M.
Comparison of active sites of some microbial ribonucleases: structural basis for guanylic specificity
Trends Biochem. Sci.
15
158-162
1990
Fungi
Yoshida, H.; Fukuda, I.; Hashiguchi, M.
Purification by affinity chromatography and physicochemical properties of the guanine-specific ribonuclease of Fusarium moniliforme
J. Biochem.
88
1813-1818
1980
Fusarium verticillioides
Nishikawa, S.; Morioka, H.; Fuchimura, K.; Tanaka, T.; Uesugi, S.; Ohtsuka, E.; Ikehara, M.
Modification of Glu 58, an amino acid of the active center of ribonuclease T1, to Gln and Asp
Biochem. Biophys. Res. Commun.
138
789-794
1986
Escherichia coli
Sevcik, J.; Lamzin, V.S.; Dauter, Z.; Wilson, K.S.
Atomic resolution data reveal flexibility in the structure of RNase Sa
Acta Crystallogr. Sect. D
58
1307-1313
2002
Kitasatospora aureofaciens (P05798), Kitasatospora aureofaciens
Jo Chitester, B.; Walz, F.G., Jr.
Kinetic studies of guanine recognition and a phosphate group subsite on ribonuclease T1 using substitution mutants at Glu46 and Lys41
Arch. Biochem. Biophys.
406
73-77
2002
Aspergillus oryzae
De Vos, S.; Backmann, J.; Prevost, M.; Steyaert, J.; Loris, R.
Hydrophobic core manipulations in ribonuclease T1
Biochemistry
40
10140-10149
2001
Aspergillus oryzae (P00651)
Kumar, K.; Walz, F.G., Jr.
Probing functional perfection in substructures of ribonuclease T1: double combinatorial random mutagenesis involving Asn43, Asn44, and Glu46 in the guanine binding loop
Biochemistry
40
3748-3757
2001
Aspergillus oryzae
Korn, K.; Wennmalm, S.; Foerster, H.H.; Hahn, U.; Rigler, R.
Analysis of the RNase T1 mediated cleavage of an immobilized gapped heteroduplex via fluorescence correlation spectroscopy
Biol. Chem.
381
259-263
2000
Aspergillus oryzae
Nonaka, G.; Ishikawa, T.; Liu, T.T.; Nakajima, H.; Kitamoto, K.
Genetic analysis of growth inhibition of yeast cells caused by expression of Aspergillus oryzae RNase T1
Biosci. Biotechnol. Biochem.
64
2152-2158
2000
Aspergillus oryzae
Loverix, S.; Winqvist, A.; Stromberg, R.; Steyaert, J.
Mechanism of RNase T1: concerted triester-like phosphoryl transfer via a catalytic three-centered hydrogen bond
Chem. Biol.
7
651-658
2000
Aspergillus oryzae
Deswarte, J.; De Vos, S.; Langhorst, U.; Steyaert, J.; Loris, R.
The contribution of metal ions to the conformational stability of ribonuclease T1: crystal versus solution
Eur. J. Biochem.
268
3993-4000
2001
Aspergillus oryzae
Huyghues-Despointes, B.M.; Thurlkill, R.L.; Daily, M.D.; Schell, D.; Briggs, J.M.; Antosiewicz, J.M.; Pace, C.N.; Scholtz, J.M.
pK values of histidine residues in ribonuclease Sa: effect of salt and net charge
J. Mol. Biol.
325
1093-1105
2003
Aspergillus oryzae
Shaw, K.L.; Grimsley, G.R.; Yakovlev, G.I.; Makarov, A.A.; Pace, C.N.
The effect of net charge on the solubility, activity, and stability of ribonuclease Sa
Protein Sci.
10
1206-1215
2001
Aspergillus oryzae
Yakovlev, G.I.; Mitkevich, V.A.; Shaw, K.L.; Trevino, S.; Newsom, S.; Pace, C.N.; Makarov, A.A.
Contribution of active site residues to the activity and thermal stability of ribonuclease Sa
Protein Sci.
12
2367-2373
2003
Aspergillus oryzae
Sevcik, J.; Dauter, Z.; Wilson, K.S.
Crystal structure reveals two alternative conformations in the active site of ribonuclease Sa2
Acta Crystallogr. Sect. D
D60
1198-1204
2004
Kitasatospora aureofaciens
Makarov, A.A.; Yakovlev, G.I.; Mitkevich, V.A.; Higgin, J.J.; Raines, R.T.
Zinc(II)-mediated inhibition of ribonuclease Sa by an N-hydroxyurea nucleotide and its basis
Biochem. Biophys. Res. Commun.
319
152-156
2004
Kitasatospora aureofaciens (P05798), Kitasatospora aureofaciens
Czaja, R.; Perbandt, M.; Betzel, C.; Hahn, U.
Purine activity of RNase T1RV is further improved by substitution of Trp59 by tyrosine
Biochem. Biophys. Res. Commun.
336
882-889
2005
Aspergillus oryzae (P00651)
Czaja, R.; Struhalla, M.; Hoschler, K.; Saenger, W.; Strater, N.; Hahn, U.
RNase T1 variant RV cleaves single-stranded RNA after purines due to specific recognition by the Asn46 side chain amide
Biochemistry
43
2854-2862
2004
Aspergillus oryzae (P00651)
Tsuji, T.; Yanagawa, H.
Foldability, enzymic activity, and interacting ability of barnase mutants obtained by permutation of secondary structure units
Biochemistry
43
6968-6975
2004
Bacillus amyloliquefaciens
Laurents, D.V.; Scholtz, J.M.; Rico, M.; Pace, C.N.; Bruix, M.
Ribonuclease Sa conformational stability studied by NMR-monitored hydrogen exchange
Biochemistry
44
7644-7655
2005
Kitasatospora aureofaciens (P05798)
Hatano, K.; Kojima, M.; Suzuki, E.; Tanokura, M.; Takahashi, K.
Determination of the NMR structure of Gln25-ribonuclease T1
Biol. Chem.
384
1173-1183
2003
Aspergillus oryzae
Stuhalla, M.; Czaja, R.; Hahn, U.
Addressing the chalange of changing the specificity of RNase T1 with rational and evolutionary approaches
ChemBioChem
5
200-205
2004
Aspergillus oryzae
Unno, K.; Juvvadi, P.R.; Nakajima, H.; Shirahige, K.; Kitamoto, K.
Identification and characterization of rns4/vps32 mutation in the RNase T1 expression-sensitive strain of Saccharomyces cerevisiae: Evidence for altered ambient response resulting in transportation of the secretory protein to vacuoles
FEMS Yeast Res.
5
801-812
2005
Aspergillus oryzae
Trevino, S.R.; Gokulan, K.; Newsom, S.; Thurlkill, R.L.; Shaw, K.L.; Mitkevich, V.A.; Makarov, A.A.; Sacchettini, J.C.; Scholtz, J.M.; Pace, C.N.
Asp79 makes a large, unfavorable contribution to the stability of RNase Sa
J. Mol. Biol.
354
967-978
2005
Kitasatospora aureofaciens (P05798)
Schrift, G.L.; Waldron, T.T.; Timmons, M.A.; Ramaswamy, S.; Kearney, W.R.; Murphy, K.P.
Molecular basis for nucleotide-binding specificity: role of the exocyclic amino group "N2" in recognition by a guanylyl-ribonuclease
J. Mol. Biol.
355
72-84
2006
Kitasatospora aureofaciens (P05798), Kitasatospora aureofaciens
Martsev, S.P.; Tsybovsky, Y.I.; Stremovskiy, O.A.; Odintsov, S.G.; Balandin, T.G.; Arosio, P.; Kravchuk, Z.I.; Deyev, S.M.
Fusion of the antiferritin antibody VL domain to barnase results in enhanced solubility and altered pH stability
Protein Eng. Des. Sel.
17
85-93
2004
Bacillus amyloliquefaciens
Giraldo, J.; De Maria, L.; Wodak, S.J.
Shift in nucleotide conformational equilibrium contributes to increased rate of catalysis of GpAp versus GpA in barnase
Proteins
56
261-276
2004
Bacillus amyloliquefaciens (P00648)
Yakovlev, G.I.; Mitkevich, V.A.; Struminskaya, N.K.; Varlamov, V.P.; Makarov, A.A.
Low molecular weight chitosan is an efficient inhibitor of ribonucleases
Biochem. Biophys. Res. Commun.
357
584-588
2007
Aspergillus oryzae, Bacillus amyloliquefaciens, Bacillus intermedius, Bos taurus, Penicillium brevicompactum, Kitasatospora aureofaciens
Kim, J.H.; Marshall, A.G.
Identification and assignment of base pairs in four helical segments of Bacillus megaterium ribosomal 5S RNA and its ribonuclease T1 cleavage fragments by means of 500-MHz proton homonuclear Overhauser enhancements
Biochemistry
29
632-640
1990
Aspergillus oryzae
Kawano, S.; Kakuta, Y.; Nakashima, T.; Kimura, M.
Crystal structures of the Nicotiana glutinosa ribonuclease NT in complex with nucleoside monophosphates
J. Biochem.
140
375-381
2006
Nicotiana glutinosa
Sakai, T.; Nakamura, N.; Umitsuki, G.; Nagai, K.; Wachi, M.
Increased production of pyruvic acid by Escherichia coli RNase G mutants in combination with cra mutations
Appl. Microbiol. Biotechnol.
76
183-192
2007
Escherichia coli
Zeller, M.E.; Csanadi, A.; Miczak, A.; Rose, T.; Bizebard, T.; Kaberdin, V.R.
Quaternary structure and biochemical properties of mycobacterial RNase E/G
Biochem. J.
403
207-215
2007
Mycobacterium tuberculosis
Ermakova, E.
Brownian dynamics simulation of the competitive reactions: binase dimerization and the association of binase and barstar
Biophys. Chem.
130
26-31
2007
Bacillus intermedius (P00649)
Spaar, A.; Dammer, C.; Gabdoulline, R.R.; Wade, R.C.; Helms, V.
Diffusional encounter of barnase and barstar
Biophys. J.
90
1913-1924
2006
Bacillus amyloliquefaciens
Alston, R.W.; Lasagna, M.; Grimsley, G.R.; Scholtz, J.M.; Reinhart, G.D.; Pace, C.N.
Tryptophan fluorescence reveals the presence of long-range interactions in the denatured state of ribonuclease Sa
Biophys. J.
94
2288-2296
2008
Kitasatospora aureofaciens
Yoshida, Y.; Tanaka, M.; Ohkuri, T.; Tanaka, Y.; Imoto, T.; Ueda, T.
Analysis of internal motions of RNase T1 complexed with a productive substrate involving 15N NMR relaxation measurements
J. Biochem.
140
43-48
2006
Aspergillus oryzae
Yin, J.; Bowen, D.; Southerland, W.M.
Barnase thermal titration via molecular dynamics simulations: Detection of early denaturation sites
J. Mol. Graph. Model.
24
233-243
2006
Bacillus amyloliquefaciens
Gu, J.; Wang, J.; Leszczynski, J.
Molecular basis of the recognition process: hydrogen-bonding patterns in the guanine primary recognition site of ribonuclease T1
J. Phys. Chem. B
110
13590-13596
2006
Aspergillus oryzae (P00651)
Horie, Y.; Ito, Y.; Ono, M.; Moriwaki, N.; Kato, H.; Hamakubo, Y.; Amano, T.; Wachi, M.; Shirai, M.; Asayama, M.
Dark-induced mRNA instability involves RNase E/G-type endoribonuclease cleavage at the AU-box and SD sequences in cyanobacteria
Mol. Genet. Genomics
278
331-346
2007
Synechocystis sp. PCC 6803
Jourdan, S.S.; McDowall, K.J.
Sensing of 5 monophosphate by Escherichia coli RNase G can significantly enhance association with RNA and stimulate the decay of functional mRNA transcripts in vivo
Mol. Microbiol.
67
102-115
2008
Escherichia coli
Roca, M.; De Maria, L.; Wodak, S.J.; Moliner, V.; Tunon, I.; Giraldo, J.
Coupling of the guanosine glycosidic bond conformation and the ribonucleotide cleavage reaction: implications for barnase catalysis
Proteins Struct. Funct. Bioinform.
70
415-428
2007
Bacillus amyloliquefaciens (P00648)
-
Makarov, A.A.; Kolchinsky, A.; Ilinskaya, O.N.
Binase and other microbial RNases as potential anticancer agents
Bioessays
30
781-790
2008
Bacillus amyloliquefaciens (P00648), Bacillus intermedius (P00649), Bacillus intermedius, Bacillus intermedius 7P (P00649)
Zasedateleva, O.A.; Mikheikin, A.L.; Turygin, A.Y.; Prokopenko, D.V.; Chudinov, A.V.; Belobritskaya, E.E.; Chechetkin, V.R.; Zasedatelev, A.S.
Gel-based oligonucleotide microarray approach to analyze protein-ssDNA binding specificity
Nucleic Acids Res.
36
e61
2008
Bacillus intermedius
Allgaier, S.; Weiland, N.; Hamad, I.; Kempken, F.
Expression of ribonuclease A and ribonuclease N1 in the filamentous fungus Neurospora crassa
Appl. Microbiol. Biotechnol.
85
1041-1049
2010
Neurospora crassa (P09646), Neurospora crassa
Moors, S.L.; Jonckheer, A.; De Maeyer, M.; Engelborghs, Y.; Ceulemans, A.
Tryptophan conformations associated with partial unfolding in ribonuclease T1
Biophys. J.
97
1778-1786
2009
Aspergillus oryzae (P00651)
Mitkevich, V.A.; Tchurikov, N.A.; Zelenikhin, P.V.; Petrushanko, I.Y.; Makarov, A.A.; Ilinskaya, O.N.
Binase cleaves cellular noncoding RNAs and affects coding mRNAs
FEBS J.
277
186-196
2010
Bacillus intermedius (P00649), Bacillus intermedius
Chung, D.H.; Min, Z.; Wang, B.C.; Kushner, S.R.
Single amino acid changes in the predicted RNase H domain of Escherichia coli RNase G lead to complementation of RNase E deletion mutants
RNA
16
1371-1385
2010
Escherichia coli
Ulyanova, V.; Vershinina, V.; Ilinskaya, O.
Barnase and binase: twins with distinct fates
FEBS J.
278
3633-3643
2011
Bacillus amyloliquefaciens, Bacillus pumilus
Dubey, S.; Kalia, Y.N.
Electrically-assisted delivery of an anionic protein across intact skin: cathodal iontophoresis of biologically active ribonuclease T1
J. Control. Release
152
356-362
2011
Aspergillus oryzae
Song, W.S.; Lee, M.; Lee, K.
RNase G participates in processing of the 5'-end of 23S ribosomal RNA
J. Microbiol.
49
508-511
2011
Escherichia coli, Escherichia coli N3433
Beauchamp, D.L.; Khajehpour, M.
Probing the effect of water-water interactions on enzyme activity with salt gradients: a case-study using ribonuclease t1
J. Phys. Chem. B
114
16918-16928
2010
Aspergillus oryzae
Kobayashi, H.; Katsutani, T.; Hara, Y.; Motoyoshi, N.; Itagaki, T.; Akita, F.; Higashiura, A.; Yamada, Y.; Inokuchi, N.; Suzuki, M.
X-ray crystallographic structure of RNase Po1 that exhibits anti-tumor activity
Biol. Pharm. Bull.
37
968-978
2014
Pleurotus ostreatus (P81762), Pleurotus ostreatus
Kobayashi, H.; Motoyoshi, N.; Itagaki, T.; Tabata, K.; Suzuki, T.; Inokuchi, N.
The inhibition of human tumor cell proliferation by RNase Pol, a member of the RNase T1 family, from Pleurotus ostreatus
Biosci. Biotechnol. Biochem.
77
1486-1491
2013
Pleurotus ostreatus (B1Q4S7), Pleurotus ostreatus
Kobayashi, H.; Motoyoshi, N.; Itagaki, T.; Suzuki, M.; Inokuchi, N.
Effect of the replacement of aspartic acid/glutamic acid residues with asparagine/glutamine residues in RNase He1 from Hericium erinaceus on inhibition of human leukemia cell line proliferation
Biosci. Biotechnol. Biochem.
79
211-217
2015
Hericium erinaceus (B1Q4V2), Hericium erinaceus, Pleurotus ostreatus (P81762), Pleurotus ostreatus
Chao, W.C.; Shen, J.Y.; Lu, J.F.; Wang, J.S.; Yang, H.C.; Wee, K.; Lin, L.J.; Kuo, Y.C.; Yang, C.H.; Weng, S.H.; Huang, H.C.; Chen, Y.H.; Chou, P.T.
Probing water environment of Trp59 in ribonuclease T1: insight of the structure-water network relationship
J. Phys. Chem. B
119
2157-2167
2014
Aspergillus oryzae
Takahashi, K.
Structure and function studies on enzymes with a catalytic carboxyl group(s): from ribonuclease T1 to carboxyl peptidases
Proc. Jpn. Acad. Ser. B Phys. Biol. Sci.
89
201-225
2013
Aspergillus oryzae
Herbert, C.; Dzowo, Y.K.; Urban, A.; Kiggins, C.N.; Resendiz, M.J.E.
Reactivity and specificity of RNase T1, RNase A, and RNase H toward oligonucleotides of RNA containing 8-oxo-7,8-dihydroguanosine
Biochemistry
57
2971-2983
2018
Aspergillus oryzae (P00651), Aspergillus oryzae ATCC 42149 (P00651)
Chao, W.; Shen, J.; Lu, J.; Wang, J.; Yang, H.; Wee, K.; Lin, L.; Kuo, Y.; Yang, C.; Weng, S.; Huang, H.; Chen, Y.; Chou, P.
Probing water environment of trp59 in ribonuclease T1 insight of the structure-water network relationship
J. Phys. Chem. B
119
2157-2167
2015
Aspergillus oryzae (P00651), Aspergillus oryzae ATCC 42149 (P00651)
-
Gerashchenko, M.V.; Gladyshev, V.N.
Ribonuclease selection for ribosome profiling
Nucleic Acids Res.
45
e6
2017
Saccharomyces cerevisiae, Caenorhabditis elegans, Drosophila melanogaster, Escherichia coli, Mus musculus, Aspergillus oryzae (P00651), Saccharomyces cerevisiae BY4741, Escherichia coli BL21alpha, Caenorhabditis elegans N2, Aspergillus oryzae ATCC 42149 (P00651)
Loverix, S.; Laus, G.; Martins, J.C.; Wyns, L.; Steyaert, J.
Reconsidering the energetics of ribonuclease catalysed RNA hydrolysis
Eur. J. Biochem.
257
286-290
1998
Aspergillus oryzae
html completed