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Information on EC - Enterobacter ribonuclease

for references in articles please use BRENDA:EC4.6.1.21
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IUBMB Comments
Preference for cleavage at Cp-A bonds. Homopolymers of A, U or G are not hydrolysed. CpG bonds are hydrolysed less well and there is no detectable hydrolysis between two purines or two pyrimidines. The enzyme catalyses a two-stage endonucleolytic cleavage. The first reaction produces 5'-hydroxy-phosphooligonucletides and 3'-phosphooligonucleotides ending a with 2',3'-cyclic phosphodiester, which are released from the enzyme. The enzyme then hydrolyses these cyclic compounds in a second reaction that takes place only when all the susceptible 3',5'-phosphodiester bonds have been cyclised. The second reaction is a reversal of the first reaction using the hydroxyl group of water instead of the 5'-hydroxyl group of ribose. The overall process is that of a phosphorus-oxygen lyase followed by hydrolysis to form the 3'-nucleotides.
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Word Map
  • contact-dependent
  • cdia-cts
  • analysis
  • two-partner
  • uropathogenic
  • auto-inhibition
  • inter-bacterial
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
Reaction Schemes
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RNA containing adenosine-cytidine
an [RNA fragment]-3'-cytidine-3'-phosphate
a 5'-a hydroxy-adenosine -3'-[RNA fragment]
CdiA-CT toxin, Enterobacter ribonuclease, more
an [RNA fragment]-3'-cytidine-2',3'-cyclophosphate + H2O = an [RNA fragment]-3'-cytidine-3'-phosphate
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RNA containing adenosine-cytidine + H2O = an [RNA fragment]-3'-cytidine-3'-phosphate + a 5'-a hydroxy-adenosine -3'-[RNA fragment]
show the reaction diagram
overall reaction
RNA containing adenosine-cytidine = an [RNA fragment]-3'-cytidine-2',3'-cyclophosphate + a 5'-a hydroxy-adenosine -3'-[RNA fragment]
show the reaction diagram
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