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Information on EC 4.6.1.20 - ribonuclease U2

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EC Tree
IUBMB Comments
The enzyme secreted by the fungus Ustilago sphaerogena cleaves at the 3'-phosphate group of purines, and catalyses a two-stage endonucleolytic cleavage. The first reaction produces 5'-hydroxy-phosphooligonucletides and 3'-phosphooligonucleotides ending in Ap or Gp with 2',3'-cyclic phosphodiester, which are released from the enzyme. The enzyme then hydrolyses these cyclic compounds in a second reaction that takes place only when all the susceptible 3',5'-phosphodiester bonds have been cyclised. The second reaction is a reversal of the first reaction using the hydroxyl group of water instead of the 5'-hydroxyl group of ribose. The overall process is that of a phosphorus-oxygen lyase followed by hydrolysis to form the 3'-nucleotides.
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Word Map
The enzyme appears in viruses and cellular organisms
Reaction Schemes
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[RNA] containing adenosine
+
=
an [RNA fragment]-3'-adenosine-3'-phosphate
+
a 5'-hydroxy-ribonucleotide-3'-[RNA fragment]
[RNA] containing guanosine
+
=
an [RNA fragment]-3'-guanosine-3'-phosphate
+
a 5'-hydroxy-ribonucleotide-3'-[RNA fragment]
Synonyms
rnase u2, ribonuclease u2, purine-specific rnase, purine-specific ribonuclease, ribonuclease u2a, ribonuclease u2b, more
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
an [RNA fragment]-3'-adenosine-2',3'-cyclophosphate + H2O = an [RNA fragment]-3'-adenosine -3'-phosphate
show the reaction diagram
(1b)
-
-
-
an [RNA fragment]-3'-guanosine-2',3'-cyclophosphate + H2O = an [RNA fragment]-3'- guanosine-3'-phosphate
show the reaction diagram
(2b)
-
-
-
[RNA] containing adenosine + H2O = an [RNA fragment]-3'-adenosine-3'-phosphate + a 5'-hydroxy-ribonucleotide-3'-[RNA fragment]
show the reaction diagram
(1) overall reaction
-
-
-
[RNA] containing adenosine = an [RNA fragment]-3'-adenosine-2',3'-cyclophosphate + a 5'-hydroxy-ribonucleotide-3'-[RNA fragment]
show the reaction diagram
(1a)
-
-
-
[RNA] containing guanosine + H2O = an [RNA fragment]-3'-guanosine-3'-phosphate + a 5'-hydroxy-ribonucleotide-3'-[RNA fragment]
show the reaction diagram
(2) overall reaction
-
-
-
[RNA] containing guanosine = an [RNA fragment]-3'-guanosine-2',3'-cyclophosphate + a 5'-hydroxy-ribonucleotide-3'-[RNA fragment]
show the reaction diagram
(2a)
-
-
-
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