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5'-(U)6-GA-(U)5-3' RNA + H2O
?
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digestion of the platinated 5'-(U)6-GA-(U)5-3' RNA shows a reaction product corresponding to [UUUUUUGA + Pt(NH3)2 + H],+ depicting RNase cleavage 3' to an adenosine predicted to be involved in a cisplatin diadduct. The platinated G*A* RNA adduct is uniquely recognized and processed by RNase U2, whereas other purine-Pt(II) adducts are not. The basis for this selectivity may arise from the RNase's known A - G preference, allowing the endonuclease in some cases to partially recognize the 3' platinated A despite platinum modification
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-
?
RNA + H2O
3'-Phosphomononucleotides + 3'-phosphooligonucleotides + 2',3'-cyclic nucleotide phosphates
RNA containing adenosine 3'-(1-naphthyl phosphate) + H2O
?
-
-
-
-
?
RNA containing adenosine 3'-(benzyl phosphate) + H2O
?
-
-
-
-
?
RNA containing adenosine 3'-(methyl phosphate) + H2O
?
-
-
-
-
?
RNA containing ApA + H2O
?
-
-
-
-
?
RNA containing ApAp + H2O
?
-
-
-
-
?
RNA containing ApC + H2O
?
-
-
-
-
?
RNA containing ApCp + H2O
?
-
-
-
-
?
RNA containing ApCpGp + H2O
?
-
-
-
-
?
RNA containing ApG + H2O
?
-
-
-
-
?
RNA containing ApGp + H2O
?
-
-
-
-
?
RNA containing ApU + H2O
?
-
-
-
-
?
RNA containing ApUp + H2O
?
-
-
-
-
?
RNA containing ApUpG + H2O
?
-
-
-
-
?
RNA containing ApUpGp + H2O
?
-
-
-
-
?
RNA containing CpApGp + H2O
?
-
-
-
-
?
RNA containing UpApGp + H2O
?
-
-
-
-
?
additional information
?
-
RNA + H2O
3'-Phosphomononucleotides + 3'-phosphooligonucleotides + 2',3'-cyclic nucleotide phosphates
-
-
-
?
RNA + H2O
3'-Phosphomononucleotides + 3'-phosphooligonucleotides + 2',3'-cyclic nucleotide phosphates
-
r, preferential cleavage at Ap or Gp
-
?
RNA + H2O
3'-Phosphomononucleotides + 3'-phosphooligonucleotides + 2',3'-cyclic nucleotide phosphates
-
r, preferential cleavage at Ap or Gp
-
?
RNA + H2O
3'-Phosphomononucleotides + 3'-phosphooligonucleotides + 2',3'-cyclic nucleotide phosphates
-
r, preferential cleavage at Ap or Gp
-
?
RNA + H2O
3'-Phosphomononucleotides + 3'-phosphooligonucleotides + 2',3'-cyclic nucleotide phosphates
-
r, preferential cleavage at Ap or Gp
-
?
RNA + H2O
3'-Phosphomononucleotides + 3'-phosphooligonucleotides + 2',3'-cyclic nucleotide phosphates
-
r, preferential cleavage at Ap or Gp
-
?
RNA + H2O
3'-Phosphomononucleotides + 3'-phosphooligonucleotides + 2',3'-cyclic nucleotide phosphates
-
r, preferential cleavage at Ap or Gp
-
?
RNA + H2O
3'-Phosphomononucleotides + 3'-phosphooligonucleotides + 2',3'-cyclic nucleotide phosphates
-
r, preferential cleavage at Ap or Gp
-
?
RNA + H2O
3'-Phosphomononucleotides + 3'-phosphooligonucleotides + 2',3'-cyclic nucleotide phosphates
-
r, preferential cleavage at Ap or Gp
-
?
RNA + H2O
3'-Phosphomononucleotides + 3'-phosphooligonucleotides + 2',3'-cyclic nucleotide phosphates
-
r, preferential cleavage at Ap or Gp
-
?
RNA + H2O
3'-Phosphomononucleotides + 3'-phosphooligonucleotides + 2',3'-cyclic nucleotide phosphates
-
r, preferential cleavage at Ap or Gp
-
?
RNA + H2O
3'-Phosphomononucleotides + 3'-phosphooligonucleotides + 2',3'-cyclic nucleotide phosphates
-
-
-
?
additional information
?
-
-
YoeB toxin has in vitro Rnase activity that preferentially cleaves at the 3-end of purine ribonucleotides
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-
?
additional information
?
-
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specifically recognizes purine ribonucleotides and catalyzes cleavage of the 3' phosphodiester bond attached to a G or an A. For platinated sequences containing XY = GG, AG, and GU, RNase U2 cleavage is not observed, being blocked at the purine nucleotides predicted to be involved in platinum binding
-
-
?
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physiological function
-
the endonuclease cleavage of many small nuclear RNAs is equally important in terminating the transcription of the U2 snRNA in Schizosaccharomyces pombe. Reversed torpedoes model for the termination and maturation of the U2 snRNA, the Pac1 endonuclease cleavage provides entry sites for the 3' and 5' exonuclease activities, leading to RNA maturation in one direction and transcript termination in the other
physiological function
-
the endonuclease cleavage of many small nuclear RNAs is equally important in terminating the transcription of the U2 snRNA in Schizosaccharomyces pombe. Reversed torpedoes model for the termination and maturation of the U2 snRNA, the Pac1 endonuclease cleavage provides entry sites for the 3' and 5' exonuclease activities, leading to RNA maturation in one direction and transcript termination in the other
-
additional information
Asn32 of RNase U2A rapidly deaminates and isomerizes in alkaline conditions, conformational mechanism, overview. In solution, the region around Asn32-Gly33 is likely to be in equilibrium between multiple conformers with deamination of Asn32 proceeding when the region adopts an extended conformation
additional information
-
Asn32 of RNase U2A rapidly deaminates and isomerizes in alkaline conditions, conformational mechanism, overview. In solution, the region around Asn32-Gly33 is likely to be in equilibrium between multiple conformers with deamination of Asn32 proceeding when the region adopts an extended conformation
additional information
-
cleavage in the U3 snoRNA transcripts of Schizosaccharomyces pombe can induce transcript termination, the degree of cleavage correlates closely with both RNA maturation and transcript termination. the RNase III-like endonuclease, Pac1, and the nuclear 50-exonuclease, Dhp1p, are essential for RNA production and transcript termination, supporting a reversed torpedoes model in which the endonuclease cut allows 5'- and 3'-exonuclease activities access to the transcript, leading simultaneously to transcript termination in one direction and RNA maturation in the other
additional information
formation of isoAsp32 induces a single turn unfolding of the alpha-helix from Asp29 to Asp34, and the region from Asp29 to Arg35 forms a U-shaped loop structure, overview. IsoAsp32 protrudes from the surface of the protein, and the abnormal beta-peptide bond in the main-chain and alpha-carboxylate in the side-chain is fully exposed. The structure suggests that the deamidation of the Asn and the isoAsp formation in proteins could confer immunogenicity
additional information
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formation of isoAsp32 induces a single turn unfolding of the alpha-helix from Asp29 to Asp34, and the region from Asp29 to Arg35 forms a U-shaped loop structure, overview. IsoAsp32 protrudes from the surface of the protein, and the abnormal beta-peptide bond in the main-chain and alpha-carboxylate in the side-chain is fully exposed. The structure suggests that the deamidation of the Asn and the isoAsp formation in proteins could confer immunogenicity
additional information
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mechanism of isoaspartate formation within the Asp45-Glu46 sequence of ribonuclease U2, overview. In the succinimide-formation reaction water 219 receives a proton from the N atom of Glu46 as a general base and waters 190 and 220 stabilize the tetrahedral intermediate, and in the succinimide-hydrolysis reaction water 219 provides a proton for the N atom of Glu46 as a general acid
additional information
-
cleavage in the U3 snoRNA transcripts of Schizosaccharomyces pombe can induce transcript termination, the degree of cleavage correlates closely with both RNA maturation and transcript termination. the RNase III-like endonuclease, Pac1, and the nuclear 50-exonuclease, Dhp1p, are essential for RNA production and transcript termination, supporting a reversed torpedoes model in which the endonuclease cut allows 5'- and 3'-exonuclease activities access to the transcript, leading simultaneously to transcript termination in one direction and RNA maturation in the other
-
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heterotrimeric complex of (YefM)2-YoeB antitoxin-toxin pair and free YoeB structure. Conformational rearrangement of YoeB RNase catalytic site upon binding of YefM
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isoAsp containing ribonuclease U2B, hanging drop vapor diffusion method, 0.004 ml of 10 mg/ml protein in 50 mM sodium citrate, pH 4.5, containing 50 mM sodium chloride, are mixed with 0.004 ml of reservoir solution containing 0.84 M sodium dihydrogen phosphate and 1.26 M dipotassium hydrogen phosphate, pH 6.9, equilibration against 0.4 ml reservoir solution, 20°C, 2 days, X-ray diffraction structure determination and analysis at 1.32 A resolution
ribonuclease U2 complexed with adenosine 3'-monophosphate, hanging drop vapour diffusion method, 0.005 ml of protein solution containing 20 mg/ml RNase U2A and 8 mM adenosine 3'-monophosphate, pH 4.5, is mixed with 0.005 ml of reservoir solution containing 12.5% w/w PEG 8000, 200 mM calcium acetate, 100 mM sodium cacodylate, pH 3.75, 12.5% w/w PEG 8000, equilibration against 0.4 ml reservoir solution, 20°C, 3 days, X-ray diffraction structure determination and analysis at 0.96-0.99 A resolution
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RNase U2A in complex with 2'-adenylic acid and Ca2+ ions, X-ray diffraction structure determination and analysis at 1.03 A resolution
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Uchida, T.; Egami, F.
Microbial ribonucleases with special reference to RNases T1, T2, N1, and U2
The Enzymes, 3rd Ed. (Boyer, P. D. , ed. )
4
205-250
1971
Ustilago sphaerogena
-
brenda
Yasuda, T.; Inoue, Y.
Studies of catalysis by ribonuclease U2. Steady-state kinetics for transphosphorylation of oligonucleotide and synthetic substrates
Biochemistry
21
364-369
1982
Ustilago sphaerogena
brenda
Uchida, T.; Shibata, Y.
An affinity adsorbent, 5-adenylate-aminohexyl-sepharose. I. Purification and properties of two forms of RNase U2
J. Biochem.
90
463-471
1981
Ustilago sphaerogena
brenda
Minato, S.; Hirai, A.
Characterization of Ustilago Ribonuclease U2. Effects of chemical modification at glutamic acid-61 and cystine-1 and of organic solvents on the enzymatic activity
J. Biochem.
85
327-334
1979
Ustilago sphaerogena
brenda
Uchida, T.; Machida, C.
The specificity for dinucleoside monophosphates of RNase U2 and urea-treated RNase U2
Nucleic Acids Res.
1 Suppl 2
409-413
1978
Ustilago sphaerogena
-
brenda
Sato, S.; Uchida, T.
Ethoxyformylation of ribonuclease U2 form Ustilago sphaerogena
J. Biochem.
77
795-800
1975
Ustilago sphaerogena
brenda
Sato, S.; Uchida, T.
On the interaction of ribonuclease U-2 and substrate analogues
Biochim. Biophys. Acta
383
168-177
1975
Ustilago sphaerogena
brenda
Kioke, T.; Uchida, T.; Egami, F.
Synthesis of adenylyl-(3,5)-nucleosides, adenylyl-(3,5)-guanosine 2,3-cyclic phosphate, and oligoadenylic acids by ribonuclease U2
J. Biochem.
69
111-117
1971
Ustilago sphaerogena
brenda
Glitz, D.G.; Dekker, C.A.
A ribonuclease from Ustilago sphaerogena. I. Purification and properties of the enzyme
Biochemistry
3
1391-1399
1964
Ustilago sphaerogena
brenda
Glitz, D.G.; Dekker, C.A.
A ribonuclease from Ustilago sphaerogena. II. Specificity of the enzyme
Biochemistry
3
1399-1406
1964
Ustilago sphaerogena
brenda
Martinez-Ruiz, A.; Garcia-Ortega, L.; Kao, R.; Onaderra, M.; Mancheno, J.M.; Davies, J.; Martinez del Pozo, A.; Gavilanes, J.G.
Ribonuclease U2: cloning, production in Pichia pastoris and affinity chromatography purification of the active recombinant protein
FEMS Microbiol. Lett.
189
165-169
2000
Ustilago sphaerogena (P00654), Ustilago sphaerogena, Ustilago sphaerogena CBS 534.71 (P00654)
brenda
Martinez-Ruiz, A.; Garcia-Ortega, L.; Kao, R.; Lacadena, J.; Onaderra, M.; Mancheno, J.M.; Davies, J.; Del Pozo, A.M.; Gavilanes, J.G.
RNase U2 and alpha-sarcin: a study of relationships
Methods Enzymol.
341
335-351
2001
Ustilago sphaerogena (P00654)
brenda
Garcia-Ortega, L.; De los Rios, V.; Martinez-Ruiz, A.; Onaderra, M.; Lacadena, J.; Martinez del Pozo, A.; Gavilanes, J.G.
Anomalous electrophoretic behavior of a very acidic protein: ribonuclease U2
Electrophoresis
26
3407-3413
2005
Ustilago sphaerogena (P00654)
brenda
Kamada, K.; Hanaoka, F.
Conformational change in the catalytic site of the ribonuclease YoeB toxin by YefM antitoxin
Mol. Cell
19
497-509
2005
Escherichia coli
brenda
Alvarez-Garcia, E.; Garcia-Ortega, L.; De los Rios, V.; Gavilanes, J.G.; Martinez-del-Pozo, A.
Influence of key residues on the heterologous extracellular production of fungal ribonuclease U2 in the yeast Pichia pastoris
Protein Expr. Purif.
65
223-229
2009
Ustilago sphaerogena (P00654), Ustilago sphaerogena
brenda
Chapman, E.G.; DeRose, V.J.
Enzymatic processing of platinated RNAs
J. Am. Chem. Soc.
132
1946-1952
2010
synthetic construct
brenda
Noguchi, S.
Isomerization mechanism of aspartate to isoaspartate implied by structures of Ustilago sphaerogena ribonuclease U2 complexed with adenosine 3'-monophosphate
Acta Crystallogr. Sect. D
66
843-849
2010
Ustilago sphaerogena
brenda
Nabavi, S.; Nazar, R.N.
Cleavage-induced termination in U2 snRNA gene expression
Biochem. Biophys. Res. Commun.
393
461-465
2010
Schizosaccharomyces pombe, Schizosaccharomyces pombe GP969
brenda
Noguchi, S.
Structural changes induced by the deamidation and isomerization of asparagine revealed by the crystal structure of Ustilago sphaerogena ribonuclease U2B
Biopolymers
93
1003-1010
2010
Ustilago sphaerogena (P00654), Ustilago sphaerogena
brenda
Nabavi, S.; Nazar, R.N.
Pac1 endonuclease and Dhp1p 5'-3' exonuclease are required for U3 snoRNA termination in Schizosaccharomyces pombe
FEBS Lett.
584
3436-3441
2010
Schizosaccharomyces pombe, Schizosaccharomyces pombe GP969
brenda
Noguchi, S.
Conformational variation revealed by the crystal structure of RNase U2A complexed with Ca ion and 2'-adenylic acid at 1.03 A resolution
Protein Pept. Lett.
17
1559-1561
2010
Ustilago sphaerogena (P00654), Ustilago sphaerogena
brenda
Houser, W.M.; Butterer, A.; Addepalli, B.; Limbach, P.A.
Combining recombinant ribonuclease U2 and protein phosphatase for RNA modification mapping by liquid chromatography-mass spectrometry
Anal. Biochem.
478
52-58
2015
synthetic construct (KP779641)
brenda
Solivio, B.; Yu, N.; Addepalli, B.; Limbach, P.A.
Improving RNA modification mapping sequence coverage by LC-MS through a nonspecific RNase U2-E49A mutant
Anal. Chim. Acta
1036
73-79
2018
Ustilago sphaerogena (P00654)
brenda