Any feedback?
Information on EC 4.6.1.2 - guanylate cyclase and Organism(s) Drosophila melanogaster and UniProt Accession Q9VEU5
for references in articles please use BRENDA:EC4.6.1.2Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
4.6.1.2 guanylate cyclaseIUBMB Comments
Also acts on ITP and dGTP.
Specify your search results
Word Map
- 4.6.1.2
- cgmp
- artery
- guanosine
- nitroprusside
- monophosphate
- phosphodiesterase
-
methylene
-
vasodilation
- endothelium
-
cgmp-dependent
-
l-name
- l-arginine
- pkg
-
tone
-
endothelium-dependent
-
blocker
- aorta
- acetylcholine
-
vasorelaxation
-
endothelium-derived
- ng-nitro-l-arginine
- snap
- phenylephrine
- 8-br-cgmp
- ng-monomethyl-l-arginine
-
endothelium-denuded
- nnos
- nomega-nitro-l-arginine
-
apamin
-
no-dependent
- zaprinast
- tetraethylammonium
- 8-bromo-cgmp
- glibenclamide
- sin-1
- s-nitroso-n-acetylpenicillamine
-
no-mediated
- sildenafil
-
no-induced
-
precontracted
- nitroglycerin
- diagnostics
- trinitrate
- molecular biology
- analysis
-
iberiotoxin
- cavernosum
- 7-nitroindazole
-
no-donors
- pharmacology
- medicine
-
phototransduction
-
charybdotoxin
-
endothelium-intact
- drug development
-
nitrergic
The taxonomic range for the selected organisms is: Drosophila melanogaster
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea
Synonyms
guanylate cyclase, soluble guanylate cyclase, guanylyl cyclase, soluble guanylyl cyclase, npr-a, npr-b, particulate guanylate cyclase, h-nox, no receptor, guanylyl cyclase c, 
more
topSYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
ANPRA
-
-
-
-
ANPRB
-
-
-
-
Atrial natriuretic peptide A-type receptor
-
-
-
-
Atrial natriuretic peptide B-type receptor
-
-
-
-
guanyl cyclase
-
-
-
-
Guanylate cyclase
-
-
-
-
Guanylate cyclase 2D, retinal
-
-
-
-
Guanylate cyclase 2E
-
-
-
-
Guanylate cyclase 2F, retinal
-
-
-
-
Guanylate cyclase, olfactory
-
-
-
-
guanylyl cyclase
-
-
-
-
Gyc-88E
Gyc-89Da
hSTAR
-
-
-
-
Intestinal guanylate cyclase
-
-
-
-
KSGC
-
-
-
-
Rod outer segment membrane guanylate cyclase
-
-
-
-
ROS-GC
-
-
-
-
ROS-GC2
-
-
-
-
soluble guanylyl cyclase
STA receptor
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
P-O bond cleavage
-
-
-
-
PATHWAY SOURCE
PATHWAYS
KEGG
SYSTEMATIC NAME
IUBMB Comments
GTP diphosphate-lyase (cyclizing; 3',5'-cyclic-GMP-forming)
Also acts on ITP and dGTP.
CAS REGISTRY NUMBER
COMMENTARY
9054-75-5
-
SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
GTP
3',5'-cyclic GMP + diphosphate
isozyme Gyc-89Db in neurons is necessary early in adult development to prevent eclosion, overview
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
isozyme Gyc-89Da in neurons is necessary early in adult development to prevent eclosion, overview
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
GTP
3',5'-cyclic GMP + diphosphate
isozyme Gyc-89Db in neurons is necessary early in adult development to prevent eclosion, overview
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
isozyme Gyc-89Da in neurons is necessary early in adult development to prevent eclosion, overview
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
tetanus toxin
expression of tetanus toxin in the neurons, that express isozyme Gyc-89Db, blocks their synaptic activity
-
1H-[1,2,4] oxadiazole [4,3-a] quinoxalin-1-one
-
effectively blocks guanylyl cyclase activity in response to low O2 levels
tetanus toxin
expression of tetanus toxin in the neurons, that express isozyme Gyc-89Da, blocks their synaptic activity
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
NO
-
conventional isoforms Gyca-99B and Gycb-100B are potently activated by NO. Atypical sGCs Gyc-88E Gyc-89Da Gyc-89Db are only slightly sensitive to NO. At atmospheric O2 concentrations, NO slightly stimulates the Gyc-88E+Gyc-89Da and Gyc-88E+Gyc-89Db subunit combinations, whereas at lower O2 concentrations NO inhibits their activity
additional information
-
none of the atypical sGCs are activated by the NO-independent, but heme-dependent, sGC stimulator BAY 41-2272
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
the isozyme Gyc-89Db is encoded in neurons that are required for larval and adult ecdyses in the fruit fly
-
in the anterior region expression of atypical sGCs in sensory neurons that innervate the dorsal and terminal organs, believed to be the main chemosensory organs of the larvae. Along the lateral body wall of larvae expressed by individual sensory neurons that express each of the subunits that appear to innervate basiconic sensilla and trachea. In the terminal segments, atypical sGCs are expressed in neurons that innervate peg sensilla on the caudal sensory cones
additional information
-
atypical sGC expressed throughout development
-
atypical sGC frequently co-localized in specific sensory neurons
the isozyme Gyc-89Da is encoded in neurons that are required for larval and adult ecdyses in the fruit fly
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). GCYDB_DROME
669
0
75882
Swiss-Prot
other Location (Reliability: 1)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
additional information
generation of an isozyme Gyc-89Db deficient mutant, reduced levels of cGMP in Gyc-89Db neurons have no effect on larval ecdysis or eclosion, but isozyme expression is necessary early in adult development to prevent eclosion, phenotype, overview
additional information
generation of an isozyme Gyc-89Db deficient mutant, reduced levels of cGMP in Gyc-89Db neurons have no effect on larval ecdysis or eclosion, but isozyme expression is necessary early in adult development to prevent eclosion, phenotype, overview
additional information
-
generation of an isozyme Gyc-89Db deficient mutant, reduced levels of cGMP in Gyc-89Db neurons have no effect on larval ecdysis or eclosion, but isozyme expression is necessary early in adult development to prevent eclosion, phenotype, overview
additional information
generation of an isozyme Gyc-89Da deficient mutant, reduced levels of cGMP in Gyc-89Da neurons have no effect on larval ecdysis or eclosion, but isozyme expression is necessary early in adult development to prevent eclosion, phenotype, overview
additional information
generation of an isozyme Gyc-89Da deficient mutant, reduced levels of cGMP in Gyc-89Da neurons have no effect on larval ecdysis or eclosion, but isozyme expression is necessary early in adult development to prevent eclosion, phenotype, overview
additional information
-
generation of an isozyme Gyc-89Da deficient mutant, reduced levels of cGMP in Gyc-89Da neurons have no effect on larval ecdysis or eclosion, but isozyme expression is necessary early in adult development to prevent eclosion, phenotype, overview
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
Gyc-88E(1-597) purified to more than 95% purity, by gel filtration and on anion-exchange column
-
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
gene encoding isozyme Gyc-89Db, expression analysis and promoter analysis, expression in Saccharomces cerevisiae
gene encoding isozyme Gyc-89Da, expression analysis and promoter analysis, expression in Saccharomces cerevisiae
Gyc-88E(1-597) expressed in S2 cells as a polyoma-tagged construct under the control of the metallothionein promoter
-
APPLICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
additional information
-
biochemical properties of Gyc-88E are unique for guanylate cyclases, the enzyme possibly functions as an oxygen sensor
additional information
-
residues H104, Y140, the YxS/TxR motif and missing cysteines Cys78 and Cys214 are conserved in all the insect atypical sGC subunits. Atypical sGCs can function as O2 sensors and can modulate sensitivity to sweet tastants
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Huang, S.H.; Rio, D.C.; Marletta, M.A.
Ligand binding and inhibition of an oxygen-sensitive soluble guanylate cyclase, Gyc-88E, from Drosophila
Biochemistry
46
15115-15122
2007
Drosophila melanogaster
Vermehren, A.; Langlais, K.K.; Morton, D.B.
Oxygen-sensitive guanylyl cyclases in insects and their potential roles in oxygen detection and in feeding behaviors
J. Insect Physiol.
52
340-348
2006
Anopheles gambiae, Apis mellifera, Bombyx mori, Caenorhabditis elegans, Drosophila melanogaster, Drosophila pseudoobscura, Manduca sexta, Tribolium castaneum, Caldanaerobacter subterraneus subsp. tengcongensis
Morton, D.B.; Stewart, J.A.; Langlais, K.K.; Clemens-Grisham, R.A.; Vermehren, A.
Synaptic transmission in neurons that express the Drosophila atypical soluble guanylyl cyclases, Gyc-89Da and Gyc-89Db, is necessary for the successful completion of larval and adult ecdysis
J. Exp. Biol.
211
1645-1656
2008
Drosophila melanogaster (Q9VEU5), Drosophila melanogaster (Q9VEU6), Drosophila melanogaster
EXTERNAL LINKS (specific for EC-Number 4.6.1.2)
Transporter Classification Database (TCDB): 1.A.87.2.9, 8.A.85.1.6, 8.A.85.1.2, 8.A.85.1.1, 8.A.85.1.3
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
