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Information on EC 4.6.1.2 - guanylate cyclase and Organism(s) Homo sapiens and UniProt Accession P51841

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IUBMB Comments
Also acts on ITP and dGTP.
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Homo sapiens
UNIPROT: P51841
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Word Map
The taxonomic range for the selected organisms is: Homo sapiens
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea
Synonyms
guanylate cyclase, soluble guanylate cyclase, guanylyl cyclase, soluble guanylyl cyclase, npr-a, npr-b, particulate guanylate cyclase, h-nox, no receptor, guanylyl cyclase c, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
membrane guanylyl cyclase receptor
-
alpha1 sGC
-
alpha1 soluble guanylyl cyclase
-
alpha1beta1 sGC
-
-
ANPRA
-
-
-
-
ANPRB
-
-
-
-
Atrial natriuretic peptide A-type receptor
-
-
-
-
Atrial natriuretic peptide B-type receptor
-
-
-
-
atrial natriuretic peptide receptor 2
-
GTP-pyrophosphate lyase
-
-
guanyl cyclase
-
-
-
-
guanyl cyclase B
-
-
Guanylate cyclase
-
-
-
-
Guanylate cyclase 2D, retinal
-
-
-
-
Guanylate cyclase 2E
-
-
-
-
Guanylate cyclase 2F, retinal
-
-
-
-
Guanylate cyclase, olfactory
-
-
-
-
guanylyl cyclase
guanylyl cyclase A
-
-
guanylyl cyclase B
-
-
guanylyl cyclase C
-
-
guanylyl cyclase C receptor
-
-
hSTAR
-
-
-
-
Intestinal guanylate cyclase
-
-
-
-
KSGC
-
-
-
-
membrane guanylyl cyclase receptor
-
membrane-bound GC
-
-
membrane-bound guanylate cyclase
-
-
nitric oxid sensitive guanylyl cyclase
-
-
NO- and haem-independent sGC
-
-
NO- and haem-independent soluble guanylate cyclase
-
-
NO-independent, heme-dependent soluble guanylate cyclase
-
-
particulate guanylate cyclase
-
-
photoreceptor guanylyl cyclase
-
-
RetGC1
-
-
retinal guanylate cyclase
-
retinal guanylyl cyclase
-
-
Rod outer segment membrane guanylate cyclase
-
-
-
-
rod outer segment membrane guanylate cyclase type 1
-
-
ROS-GC
-
-
-
-
ROS-GC1
-
-
ROS-GC2
-
-
-
-
soluble guanylate cyclase
soluble guanylyl cyclase
STA receptor
-
-
-
-
additional information
-
the enzyme is a member of the family of nucleotide cyclizing enzymes
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
GTP = 3',5'-cyclic GMP + diphosphate
show the reaction diagram
reaction mechanism, structure-function relationship
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
P-O bond cleavage
-
-
-
-
PATHWAY SOURCE
PATHWAYS
SYSTEMATIC NAME
IUBMB Comments
GTP diphosphate-lyase (cyclizing; 3',5'-cyclic-GMP-forming)
Also acts on ITP and dGTP.
CAS REGISTRY NUMBER
COMMENTARY hide
9054-75-5
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
2'-deoxy-GTP
?
show the reaction diagram
-
effective substrate
-
-
?
2'-O-(N-methylanthraniloyl) guanosine 5'-triphosphate
?
show the reaction diagram
-
-
-
-
?
CTP
3',5'-cyclic CMP + diphosphate
show the reaction diagram
-
-
-
-
?
GTP
3',5'-cGMP + diphosphate
show the reaction diagram
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
GTP
3',5'-cyclic-GMP + diphosphate
show the reaction diagram
-
-
-
-
?
UTP
3',5'-cyclic UMP + diphosphate
show the reaction diagram
-
-
-
-
?
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
guanylyl cyclase receptors synthesize the second-messenger cyclic GMP
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
GTP
3',5'-cyclic-GMP + diphosphate
show the reaction diagram
-
-
-
-
?
additional information
?
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
ATP
-
ATP activates guanylyl cyclase isoforms GC-A and GC-B. 0.01 mM Go6976 increases the potency of ATP for isoform GC-B 4fold
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
-
modulates the membrane guanylate cyclase transduction machinery by both inhibiting and stimulating it involving the guanylate cyclae, the guanylate cyclase activating protein type 1, S100B, and neurocalcin delta, mechanism, overview
CO
-
carbon monoxide is a modulator of neurotransmission in cardiac ganglia and in neural control of the adult human heart
Fe2+
-
ligands of the form XO can bind to the heme Fe in either a linear or bent configuration
additional information
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1H-(1,2,4)-oxadiazole-(4,3-a)quinoxalin-1-one
-
-
1H-(1,2,4)oxadiazole(4,3-a)quinoxalin-1one
1H-[1,2,4]oxadiazolo[4,3,-a]quinoxalin-1-one
i.e. ODQ
1H-[1,2,4]oxadiazolo[4,3-a]-quinoxalin-1-one
-
guanylyl cyclase inhibitor, completely abrogates chemotactic and chemokinetic cellular movement to both diethylamine/NO and diethylenetriamine/NO exposure
1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one
1H-[1,2,4]oxadiazolo[4.3a]quinoxalin-1-one
-
inhibition of the enzyme is blocked by 8-bromo-cGMP or L-arginine
2',3'-O-(2,4,6-trinitrophenyl)-ADP
-
-
2',3'-O-(2,4,6-trinitrophenyl)-AMP
-
-
2',3'-O-(2,4,6-trinitrophenyl)-ATP
-
-
2',3'-O-(2,4,6-trinitrophenyl)-CTP
-
-
2',3'-O-(2,4,6-trinitrophenyl)-GDP
-
-
2',3'-O-(2,4,6-trinitrophenyl)-GTP
-
-
2',3'-O-(2,4,6-trinitrophenyl)-UTP
-
-
3',5'-cyclic GMP
20 mM, 20-30% competitive inhibition
4H-8-bromo-1,2,4-oxadiazolo(3,4-d)benz(b)(1,4)oxazin-1-one
6-anilino-5,8-quinolinedione
-
-
8-aza-3ATP
-
inhibition of basal and sodium nitroprusside activated sGC activity
8-bromo-4H-[1,2,4]oxadiazolo[3,4-c][1,4]benzoxazin-1-one
-
i.e. NS-2028, a selective sGC inhibitor, 95% inhibition of enzyme activity at 0.01 mM, inhibits cell proliferation
Ba2+
-
-
BaY 63-2521
-
the sGC inhibitor gives apositive effect in severe pulmonary hypertention patients
Co2+
-
-
Cu2+
-
-
diphosphate
competitive inhibition
Fe2+
-
-
Fe3+
-
-
Go6976
-
competitive inhibitor. Go6976 reduces GTP binding to the catalytic site of isoforms GC-A and GC-B. Inhibition of isoform GC-B is minimal in the absence of ATP and 1 mM ATP increases the inhibition 4fold
guanylyl cyclase activating protein 1
-
GCAP1, a Ca2+/Mg2+-binding protein, metal binding in EF-hand 4 has no role in the primary attachment of GCAP1 to the cyclase, it only triggers the activator-to-inhibitor functional switch in GCAP1, differential binding of GCAP1 mutants, e.g. E75Q/E111Q/E155Q or D100N/D102G mutants, to RetGC1 in HEK-293 cells, overview
-
Hg2+
-
-
methylene blue
-
-
Pb2+
-
-
S-nitrosocysteine
-
inhibition of sGC activity by S-nitrosocysteine occurs only in parallel with the loss of cellular glutathione suggesting that loss of sGC activity may occur as a result of the severe depletion of the reduced thiol pool that occurs after exposure of cells to S-nitrosocysteine
sGC alpha1 splice variant
inhibits the enzyme, no co-precipitation of the N1-alpha1 sGC with the beta-subunit
-
superoxide
-
-
thrombospondin-1
-
thrombospondin-1 is a universal inhibitor of sGC, blocking both hemedependent and heme-independent activation
-
[1,2,4]-oxadiazolo[4,3-a]quinoxalin-1-one
additional information
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2'-deoxy-ATP
-
poor allosteric activator
2'-deoxy-GTP
-
poor allosteric activator
2,2-diethyl-1-nitroso-oxyhydrazine
2-[1-[(2-fluorophenyl)methyl]-1H-pyrazolo[3,4-b]pyridin-3-yl]-5(4-morpholinyl)-4,6-pyrimidinediamine
-
trivial name BAY 41-8543, 66fold activation at 0.1 mM, strong synergistic activation in the presence of NO
3-(5'-hydroxymethyl-2'-furyl)-1-benzylindazole
-
0.2 mM, 80fold activation of recombinant sGC
3-(5'-hydroxymethyl-2'furyl)-1-benzyl-indazole
0.1 mM, approx. 50fold stimulation, more than 2000fold stimulation in the presence of 0.1 mM sodium prusside
3-ethyl-3-(ethylaminoethyl)-1-hydroxy-2-oxo-1-triazene
-
i.e. NOC-12
4-[((4-carboxybutyl){2-[(4-phenethylbenzol)oxy]phenethyl}amino)methyl] benzoic acid
-
trivial name BAY 58-2667, heme-independent activator, 30fold activation at 0.01 mM
A-350619
-
a heme-dependent stimulator of sGC, structurally not related to YC-1, but synergistic to YC-1 and sodium nitroprusside for the binding site
A-778935
-
i.e. cis-3-[2-(2,2-dimethyl-propylsulfanyl)pyridin-3-yl]-N-(3-hydroxycyclohexyl-)acrylamide, derived from the YC-1 structure, activates the enzyme is a synergistic fashion with the NO donor sodium nitroprusside
arachidonic acid peroxide
-
activation
ATP
-
ATP binding to the allosteric site is essential for the activation of isoform GC-B under physiologic condition. ATP increases the activities 2.4fold for isoform GC-A and 1.4fold for isoform GC-B
atrial natriuretic peptide
-
BAY 41-2272
BAY 41-8543
BAY 58-2667
BAY 60-2770
-
activator BAY 60-2770 has higher efficiency on the purified protein than BAY 58-2667, i.e. cinaciguat
BaY 63-2521
BAY41-2272
i.e. 5-cyclopropyl-2-[1-(2-fluoro-benzyl)-1H-pyrazolo[3,4-b]pyridin-3-yl]-pyrimidin-4-ylamine
brain natriuretic peptide
-
activation of isoforms GC-A and GC-B
-
C-type natriuretic peptide
-
activation of isoforms GC-A and GC-B
-
CFM-1571
-
stimulator of the NO-independent, heme-dependent soluble guanylate cyclase, acts independently of and in synergism with NO producing anti-aggregatory, anti-proliferative, and vasodilatory effects, overview
cGMP
-
activation
cinaciguat
diethylamine/NO
-
increases chemotaxis
diethylenetriamine/NO
-
increases chemotaxis
G protein alpha subunit
-
-
-
GCAP1
activating protein. At near-saturating concentrations, GCAP1 and GCAP2 activate RetGC1 from HEK293 in a non-additive fashion. GCAP1 mutant M26R binds but does not activate RetGC1 and suppresses activation of recombinant and native RetGC1 by competing with both GCAP1 and GCAP2
-
GCAP2
activating protein. At near-saturating concentrations, GCAP1 and GCAP2 activate RetGC1 from HEK293 in a non-additive fashion. Deletion of a residues Tyr1016-Ser1103 fragment in RetGC1 does not block GCAP2 binding to the cyclase. Substitutions in the kinase homology domain, W708R and I734T, linked to Leber congenital amaurosis prevent binding of both GCAP1-GFP and GCAP2-GFP
-
guanylin
-
activation of isoform GC-C
guanylyl cyclase activating protein 1
-
GCAP1, a Ca2+/Mg2+-binding protein, after substitution of Ca2+ by Mg2+ in its EF-hands, stimulates photoreceptor guanylyl cyclase, RetGC1, in response to light. Metal binding in EF-hand 2 is crucial for GCAP1 attachment to RetGC1, while in EF-hand 3 it is less critical, although it enhances the efficiency of the GCAP1 docking on the target enzyme. Metal binding in EF-hand 4 has no role in the primary attachment of GCAP1 to the cyclase, it only triggers the activator-to-inhibitor functional switch in GCAP1, differential binding of GCAP1 mutants, e.g. E75Q/E111Q/E155Q or D100N/D102G mutants, to RetGC1 in HEK-293 cells, overview
-
HMR 1766
-
activates the NO- and haem-independent soluble guanylate cyclase, compound is capable of selectively activating the oxidized/haem-free enzyme via binding to the enzyme's haem pocket, causing pronounced vasodilatation
meso-porphyrin IX
-
heme-independent activator
natriuretic peptide
-
-
-
nitric oxide
riociguat
-
i.e. BAY 63-2521, a soluble guanylate cyclase stimulator, acts directly, stimulates the enzyme, and increases the sensitivity to low NO levels. It significantly improves pulmonary haemodynamic parameters and cardiac index in patients with pulmonary hypertension
S-nitrosocysteine
-
activates soluble guanylyl cyclase at low concentration (0.02 mM), oxy-hemoglobin inhibits the ability of S-nitrosocysteine to activate sGC
sodium nitroprusside
von Willebrand factor/ristocetin
-
increases the enzyme activity in an NO-independent manner correlating with Src kinase-dependent phosphorylation of sGC beta1-subunit-Tyr192
-
additional information
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0357
2'-O-(N-methylanthraniloyl) guanosine 5'-triphosphate
-
-
0.0498 - 0.887
GTP
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.15 - 28.7
GTP
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00071
2',3'-O-(2,4,6-trinitrophenyl)-ATP
-
in 10 mM MnCl2, 100 mM KCl, 25 mM HEPES/NaOH, pH 7.4, at 30°C
0.0051
2',3'-O-(2,4,6-trinitrophenyl)-CTP
-
in 10 mM MnCl2, 100 mM KCl, 25 mM HEPES/NaOH, pH 7.4, at 30°C
0.0072
2',3'-O-(2,4,6-trinitrophenyl)-GTP
-
in 10 mM MnCl2, 100 mM KCl, 25 mM HEPES/NaOH, pH 7.4, at 30°C
0.00311
2',3'-O-(2,4,6-trinitrophenyl)-UTP
-
in 10 mM MnCl2, 100 mM KCl, 25 mM HEPES/NaOH, pH 7.4, at 30°C
2.3
ATP
-
37°C, pH 7.5
0.3
diphosphate
-
0.001 - 0.0013
Go6976
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.002
Go6976
Homo sapiens
-
in the presence of 1 mM ATP, isozyme GC-B, in 25 mM HEPES (pH 7.4), 50 mM NaCl, 0.1% (w/v) bovine serum albumin, 0.5 mM isobutylmethyl xanthine, 1 mM EDTA, 0.5 mM microcystin and 5 mM MgCl2, at 37°C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0056
basal sGC activity
0.0096
-
recombinant intracellular domain of GCC
0.034
-
basal activity in the absence of NO
0.205
-
-
0.94
-
-
2.96
-
in the presence of 0.1 mM 2,2-diethyl-1-nitroso-oxyhydrazine as NO source
22.1
-
NO-stimulated sGC
3.25
sodium nitroprusside activated sGC activity
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.4
assay at
7.5
-
assay at
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
isozyme GC-F; isozyme GC-F
UniProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
isozymes GC-E and GC-F are colocalized within the same photoreceptor cells of the retina
Manually annotated by BRENDA team
-
cardiac, tissue localization, overview
Manually annotated by BRENDA team
-
right and left
Manually annotated by BRENDA team
-
expression of alpha1 and beta1 subunits of sGC
Manually annotated by BRENDA team
-
very low expression of alpha1 and beta1 subunits of sGC
Manually annotated by BRENDA team
isozymes GC-E and GC-F are colocalized within the same photoreceptor cells of the retina
Manually annotated by BRENDA team
-
ROS-GC1
Manually annotated by BRENDA team
-
pulmonary artery smooth muscle layer
Manually annotated by BRENDA team
-
atrial natriuretic peptide stimulated membrane-bound GC and guanylin-stimulated GC-C
Manually annotated by BRENDA team
-
colon cancer cell line
Manually annotated by BRENDA team
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
-
inhibition of soluble guanylyl cyclase reduces vascular endothelial growth factor-induced angiogenesis and permeability
physiological function
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
GUC2F_HUMAN
1108
2
124850
Swiss-Prot
Mitochondrion (Reliability: 5)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
120000
-
x * 120000, SDS-PAGE
135000 - 160000
-
soluble form, HPLC
155000
gel filtration, recombinant His-tagged sGC
160000
-
gel filtration
180000
-
gel filtration
70000
71000
-
1 * 83000 + 1 * 71000, SDS-PAGE
72000
-
1 * 72000 + 1 * 80000, SDS-PAGE
73000
alpha,beta 1 * 73000 + 1 * 70000, SDS-PAGE, soluble form
77000
-
1 * 77000, alpha-subunit, + 1 * 70000, beta-subunit, SDS-PAGE
80000
-
1 * 72000 + 1 * 80000, SDS-PAGE
83000
-
1 * 83000 + 1 * 71000, SDS-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
-
x * 120000, SDS-PAGE
dimer
heterodimer
additional information
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glycoprotein
phosphoprotein
-
activation by Src kinase-dependent phosphorylation of sGC beta1-subunit-Tyr192
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
homology modeling of the catalytic domain in inactive or active conformations based on PDB entries 2WZ1, 3ET6, 1CJU and molecular dynamics simulations reveals presence of potential high-affinity binding site on the active structure. The site is located between the pseudo-symmetric and the catalytic site just over the loop beta2-beta3 and does not overlap with the forskolin binding site on adenylate cyclases
sitting drop vapor diffusion method, using 0.05 M KH2PO4 and 20% (w/v) PEG 8000
sitting drop vapor diffusion method, using 20% (w/v) PEG3350, 50 mM HEPES (pH 7.0), and 1% (w/v) tryptone
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D100N/D102G
-
the mutant shows altered binding of guanylyl cyclase activating protein 1 compared to the wild-type enzyme
D530A
-
mutation in alpha subunit. Protein levels of the catalytically inactive, non-nucleotide binding mutant a1/b1 are not affected by activator drugs
DELTAN364
-
alpha1 DELTAN364 deletion mutant shows a complete loss of sensitivity towards NO or 3-(5'-hydroxymethyl-2'-furyl)-1-benzylindazole and a slight decrease in basal sGC activity
E75Q/E111Q/E155Q
-
the mutant shows altered binding of guanylyl cyclase activating protein 1 compared to the wild-type enzyme
G959A
missense mutation, mutant binds C-type natriuretic peptide on the surface of cells but fails to synthesize cGMP in membrane guanylate cyclase assays. Mutant protein is dephosphorylated and incompletely glycosylated
H105C
-
heme-deficient mutant, 70 times higher basal sGC activity than wild-type, basal activity is not affected by NO, heme reconstituted mutant regains No activation
I734T
substitution in the kinase homology domain linked to Leber congenital amaurosis, prevents binding of both GCAP1-GFP and GCAP2-GFP
L658F
missense mutation, mutant binds C-type natriuretic peptide on the surface of cells but fails to synthesize cGMP in membrane guanylate cyclase assays. Mutant protein is dephosphorylated and incompletely glycosylated
R776W
missense mutation, mutant binds C-type natriuretic peptide on the surface of cells but fails to synthesize cGMP in membrane guanylate cyclase assays. Mutant protein is dephosphorylated and incompletely glycosylated
S473A
mutation of potential phosphorylation site, reduces vmax value by 13-55%
S473E
mutation of potential phosphorylation site, reduces vmax value by 13-55%
S473E/S497E/T500E/S502E/S506E/S510E/T513E
2fold increase of Km value compared to wild-type
S487A
mutation of potential phosphorylation site, reduces vmax value by 13-55%
S487E
mutation of potential phosphorylation site, reduces vmax value by 13-55%
S497A
mutation of potential phosphorylation site, mutation increases Km value about 4fold
S497E
mutation of potential phosphorylation site, reduces vmax value by 13-55%
S497E/T500E/S502E/S506E/S510E/T513E
mutations in potential phosphorylation sites, about 20% of the activity of phosphorylated wild-type
S502A
mutation of potential phosphorylation site, reduces vmax value by 13-55%
S502E
mutation of potential phosphorylation site, reduces vmax value by 13-55%
S506A
mutation of potential phosphorylation site, reduces vmax value by 13-55%
S506E
mutation of potential phosphorylation site, reduces vmax value by 13-55%
S510A
mutation of potential phosphorylation site, reduces vmax value by 13-55%
S510E
mutation of potential phosphorylation site, reduces vmax value by 13-55%
T500A
mutation of potential phosphorylation site, reduces vmax value by 13-55%
T500E
mutation of potential phosphorylation site, reduces vmax value by 13-55%
T513A
mutation of potential phosphorylation site, reduces vmax value by 13-55%
T513E
mutation of potential phosphorylation site, reduces vmax value by 13-55%
W708R
substitution in the kinase homology domain linked to Leber congenital amaurosis, prevents binding of both GCAP1-GFP and GCAP2-GFP
Y708C
missense mutation, mutant binds C-type natriuretic peptide on the surface of cells but fails to synthesize cGMP in membrane guanylate cyclase assays. Mutant protein is dephosphorylated and incompletely glycosylated
additional information
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
glutathione-Sepharose 4B resin column chromatography, Q-Sepharose column chromatography, and Talon resin column chromatography
-
Ni-NTA column chromatography, Ni-IDA column chromatography, and Superdex 75 gel filtration
nickel HisTrap affinity column chromatography, HiTrap column chromatography, and Superdex 200 or Superdex 75 gel filtration
partial
-
Q-Sepharose, Blue-Sepharose, hydroxyapatite, gel filtration
-
recombinant GST-tagged retGC from COS-7 cells by glutathione affinity chromatography
recombinant intracellular domain of GCC
-
recombinant sGC
recombinant sGC, ion-exchange, hydroxyapatite, gel filtration
-
recombinant soluble guanylate cyclase from Spodoptera frugiperda SF9 cells by anion exchange chromatography, nickel affinity chromatography, and again anion exchange chromatography on a porus resin
-
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
by baculovirus expression system
-
construction of multiple N- and C-terminal deletion variants and cotransfecting them with full-length alpha1 subunit into COS cells
-
enzyme expression in heart tissue and heterogeneous expression patterns of NO-related regulatory enzyme systems, overview
-
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli BL21(DE3)-R3 cells
expressed in HEK-293T cells
-
expressed in Sf9 insect cells
-
expression analysis of sGC subunits in pulmonary artery tissue from healthy and hypertensive lungs
-
expression in HEK-293 cell
expression in Sf9 insect cells
-
expression of alpha1 and beta1 subunits in Sf9 insect cells
-
expression of alpha1 and beta1 subunits of sGC in Sf9 insect cells
expression of His-tagged soluble guanylate cyclase in Spodoptera frugiperda SF9 cells using the baculovirus transfection method
-
expression of isoform alpha2,beta1 in Sf9 cells
expression of sGC in BE2 human neuroblastoma cell line and in Spodoptera frugiperda SF9 cells, quantitative expression analysis of sGC splice variants, overview
expression of sGC in insect cells
-
expression of sGCalpha and sGCbeta subunit in Sf1 insect cells
-
expression of the intracellular domain of GCC in Sf21 insect cells
-
expression of wild-type GFP-tagged RetGC1 and commercially available dsRet-tagged RetGC1 in HEK-293 cells, co-expression with either fluorescently tagged or non-tagged guanylyl cyclase activating protein 1, with inactivated metal binding in individual EF-hands, in cell membranes. The uniform cellular distribution of GCAP1, also in the nucleus, drastically changes when the cells express both GCAP1-GFP and RetGC1, GCAP1 is then depleted from the nuclei and only observed in the cytoplasm of the cells, overview
-
GC-A gene, genotyping of hypertensive and normotensive Japanese, association of CT dinucleotide repeat polymorphism in the 5'-flanking region of the GC-A gene with essential hypertension in the Japanese, overview
-
isolation of two mRNA species for sGC beta2 with dissimilar 5'-untranslated regions from human kidney, translational mechanism of the sGC beta2-subunit, overview. Insertion of these regions between the two luciferase genes of a bicistronic vector and transfection into HeLa cells, both sGC beta2 leaders have internal ribosome entry site, IRES, activity in a cell-type dependent manner. The sGC beta2 IRES is functional in a wide range of cell lines, e.g. HeLa cells, Hep-G2 cells, COS-7 cells, and CCL-185 cells
isozymes GC-D, GC-E, to GC-G
quantitative analysis of promoter activity and determination of transcriptional start sites within the 5'-flanking region of alpha1 and beta1 sGC using transiently transfected luciferase reporter constructs, expression in human aortic smooth muscle cells and in COS-7 cells
-
transfection of HEK-293T cell
transfection of Sf9 cells
-
transient expression in COS-7 cells as GST-tagged enzyme, reconstitution of the Galphat-retGC interaction in COS-7 cells, overview
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
diagnostics
-
CT and GA polymorphisms in the 5'-flanking region of the GC-A gene might be useful as susceptibility marker for hypertension
drug development
-
the soluble guanylate cyclase is a target for compounds used in therapy of heart failure, e.g. nitric oxide-independent, soluble guanylate cyclase activator cinaciguat, BAY 58-2667, that induces vasodilation preferentially in diseased vessels, overview
medicine
pharmacology
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Asano, T.; Hidaka, H.
Purification of guanylate cyclase from human platelets and effect of arachidonic acid peroxide
Biochem. Biophys. Res. Commun.
78
910-918
1977
Homo sapiens
Manually annotated by BRENDA team
Boehme, E.; Jung, R.; Mechler, I.
Guanylate cyclase in human platelets
Methods Enzymol.
38C
199-202
1974
Homo sapiens
-
Manually annotated by BRENDA team
Russwurm, M.; Behrends, S.; Harteneck, C.; Koesling, D.
Functional properties of a naturally occurring isoform of soluble guanylyl cyclase
Biochem. J.
335
125-130
1998
Homo sapiens (P33402), Homo sapiens
Manually annotated by BRENDA team
Bruene, B.; Schmidt, K.U.; Ullrich, V.
Activation of soluble guanylate cyclase by carbon monoxide and inhibition by superoxide anion
Eur. J. Biochem.
192
683-688
1990
Homo sapiens
Manually annotated by BRENDA team
Chhajlani, V.; Axelsson, K.; Ahlner, J.; Wikberg, J.E.S.
Purification of soluble guanylate cyclase enzyme from human platelets
Biochem. Int.
19
1039-1044
1989
Homo sapiens
Manually annotated by BRENDA team
Kosarikov, D.N.; Young, P.; Uversky, V.N.; Gerber, N.C.
Human soluble guanylate cyclase: functional expression, purification and structural characterization
Arch. Biochem. Biophys.
388
185-197
2001
Homo sapiens
Manually annotated by BRENDA team
Koglin, M.; Behrends, S.
Native human nitric oxide sensitive guanylyl cyclase: purification and characterization
Biochem. Pharmacol.
67
1579-1585
2004
Homo sapiens
Manually annotated by BRENDA team
Vijayachandra, K.; Guruprasad, M.; Bhandari, R.; Manjunath, U.H.; Somesh, B.P.; Srinivasan, N.; Suguna, K.; Visweswariah, S.S.
Biochemical characterization of the intracellular domain of the human guanylyl cyclase C receptor provides evidence for a catalytically active homotrimer
Biochemistry
39
16075-16083
2000
Homo sapiens
Manually annotated by BRENDA team
Schmidt, P.; Schramm, M.; Schroder, H.; Stasch, J.P.
Mechanisms of nitric oxide independent activation of soluble guanylyl cyclase
Eur. J. Pharmacol.
468
167-174
2003
Homo sapiens
Manually annotated by BRENDA team
Koglin, M.; Behrends, S.
A Functional domain of the a1 subunit of soluble guanylyl cyclase is necessary for activation of the enzyme by nitric oxide and YC-1 but is not involved in heme binding
J. Biol. Chem.
278
12590-12597
2003
Homo sapiens
Manually annotated by BRENDA team
Rambotti, M.G.; Spreca, A.; Giambanco, I.; Sorci, G.; Donato, R.
Ultracytochemistry as a tool for the study of the cellular and subcellular localization of membrane-bound guanylate cyclase (GC) activity. Applicability to both receptor-activated and receptor-independent GC activity
Mol. Cell. Biochem.
230
85-96
2002
Oryctolagus cuniculus, Homo sapiens, Rattus norvegicus
Manually annotated by BRENDA team
Martin, E.; Sharina, I.; Kots, A.; Murad, F.
A constitutively activated mutant of human soluble guanylyl cyclase (sGC): Implication for the mechanism of sGC activation
Proc. Natl. Acad. Sci. USA
100
9208-9213
2003
Homo sapiens
Manually annotated by BRENDA team
Ruiz-Stewart, I.; Tiyyagura, S.R.; Lin, J.E.; Kazerounian, S.; Pitari, G.M.; Schulz, S.; Martin, E.; Murad, F.; Waldman, S.A.
Guanylyl cyclase is an ATP sensor coupling nitric oxide signaling to cell metabolism
Proc. Natl. Acad. Sci. USA
101
37-42
2004
Homo sapiens
Manually annotated by BRENDA team
Lee, Y.C.; Martin, E.; Murad, F.
Human recombinant soluble guanylyl cyclase: expression, purification, and regulation
Proc. Natl. Acad. Sci. USA
97
10763-10768
2000
Homo sapiens (Q02108), Homo sapiens
Manually annotated by BRENDA team
Zhou, Z.; Gross, S.; Roussos, C.; Meurer, S.; Muller-Esterl, W.; Papapetropoulos, A.
Structural and functional characterization of the dimerization region of soluble guanylyl cyclase
J. Biol. Chem.
279
24935-24943
2004
Homo sapiens
Manually annotated by BRENDA team
Madhani, M.; Okorie, M.; Hobbs, A.J.; MacAllister, R.J.
Reciprocal regulation of human soluble and particulate guanylate cyclases in vivo
Br. J. Pharmacol.
149
797-801
2006
Homo sapiens, Mus musculus, Mus musculus C57BL/6
Manually annotated by BRENDA team
Poulos, T.L.
Soluble guanylate cyclase
Curr. Opin. Struct. Biol.
16
736-743
2006
Bos taurus, Clostridium botulinum, Homo sapiens, Rattus norvegicus, Caldanaerobacter subterraneus subsp. tengcongensis, Vibrio cholerae serotype O1
Manually annotated by BRENDA team
Isenberg, J.S.; Ridnour, L.A.; Thomas, D.D.; Wink, D.A.; Roberts, D.D.; Espey, M.G.
Guanylyl cyclase-dependent chemotaxis of endothelial cells in response to nitric oxide gradients
Free Radic. Biol. Med.
40
1028-1033
2006
Homo sapiens
Manually annotated by BRENDA team
Pitari, G.M.; Li, T.; Baksh, R.I.; Waldman, S.A.
Exisulind and guanylyl cyclase C induce distinct antineoplastic signaling mechanisms in human colon cancer cells
Mol. Cancer Ther.
5
1190-1196
2006
Homo sapiens
Manually annotated by BRENDA team
Singh, S.; Gray, T.; Wurster, R.D.
Nitric oxide and carbon monoxide synthesizing enzymes and soluble guanylyl cyclase within neurons of adult human cardiac ganglia
Auton. Neurosci.
145
93-98
2009
Homo sapiens
Manually annotated by BRENDA team
Rosenzweig, D.H.; Nair, K.S.; Levay, K.; Peshenko, I.V.; Crabb, J.W.; Dizhoor, A.M.; Slepak, V.Z.
Interaction of retinal guanylate cyclase with the alpha subunit of transducin: potential role in transducin localization
Biochem. J.
417
803-812
2009
Bos taurus, Mus musculus, Homo sapiens (Q02846)
Manually annotated by BRENDA team
Tian, X.; Michal, A.M.; Li, P.; Wolfe, H.R.; Waldman, S.A.; Wickstrom, E.
STa peptide analogs for probing guanylyl cyclase C
Biopolymers
90
713-723
2008
Homo sapiens, Mus musculus
Manually annotated by BRENDA team
Haramis, G.; Zhou, Z.; Pyriochou, A.; Koutsilieris, M.; Roussos, C.; Papapetropoulos, A.
cGMP-independent anti-tumour actions of the inhibitor of soluble guanylyl cyclase, ODQ, in prostate cancer cell lines
Br. J. Pharmacol.
155
804-813
2008
Homo sapiens
Manually annotated by BRENDA team
Hoenicka, M.; Schmid, C.
Cardiovascular effects of modulators of soluble guanylyl cyclase activity
Cardiovasc. Hematol. Agents Med. Chem.
6
287-301
2008
Bos taurus, Canis lupus familiaris, Oryctolagus cuniculus, Homo sapiens, Mus musculus, Rattus norvegicus, Sus scrofa, Ovis aries (Q8SPV3)
Manually annotated by BRENDA team
Brahmajothi, M.V.; Campbell, D.L.
Heterogeneous expression of NO-activated soluble guanylyl cyclase in mammalian heart: implications for NO- and redox-mediated indirect versus direct regulation of cardiac ion channel function
Channels
1
353-365
2008
Homo sapiens, Mustela putorius furo
Manually annotated by BRENDA team
Schermuly, R.T.; Stasch, J.P.; Pullamsetti, S.S.; Middendorff, R.; Mueller, D.; Schlueter, K.D.; Dingendorf, A.; Hackemack, S.; Kolosionek, E.; Kaulen, C.; Dumitrascu, R.; Weissmann, N.; Mittendorf, J.; Klepetko, W.; Seeger, W.; Ghofrani, H.A.; Grimminger, F.
Expression and function of soluble guanylate cyclase in pulmonary arterial hypertension
Eur. Respir. J.
32
881-891
2008
Homo sapiens, Mus musculus, Rattus norvegicus
Manually annotated by BRENDA team
Grimminger, F.; Weimann, G.; Frey, R.; Voswinckel, R.; Thamm, M.; Boelkow, D.; Weissmann, N.; Mueck, W.; Unger, S.; Wensing, G.; Schermuly, R.T.; Ghofrani, H.A.
First acute haemodynamic study of soluble guanylate cyclase stimulator riociguat in pulmonary hypertension
Eur. Respir. J.
33
785-792
2009
Homo sapiens
Manually annotated by BRENDA team
Vazquez-Padron, R.I.; Pham, S.M.; Mateu, D.; Khan, S.; Aitouche, A.
An internal ribosome entry site mediates the initiation of soluble guanylyl cyclase beta2 mRNA translation
FEBS J.
275
3598-3607
2008
Homo sapiens (O75343), Homo sapiens
Manually annotated by BRENDA team
Jankowska, A.; Burczy?ska, B.; Duda, T.; Warchol, J.B.
Rod outer segment membrane guanylate cyclase type 1 (ROS-GC1) calcium-modulated transduction system in the sperm
Fertil. Steril.
93
904-912
2008
Homo sapiens
Manually annotated by BRENDA team
Stasch, J.P.; Hobbs, A.J.
NO-independent, haem-dependent soluble guanylate cyclase stimulators
Handb. Exp. Pharmacol.
191
277-308
2009
Homo sapiens
Manually annotated by BRENDA team
Schmidt, H.H.; Schmidt, P.M.; Stasch, J.P.
NO- and haem-independent soluble guanylate cyclase activators
Handb. Exp. Pharmacol.
191
309-339
2009
Homo sapiens
Manually annotated by BRENDA team
Kuhn, M.
Function and dysfunction of mammalian membrane guanylyl cyclase receptors: lessons from genetic mouse models and implications for human diseases
Handb. Exp. Pharmacol.
191
47-69
2009
Homo sapiens, Homo sapiens (P16066), Homo sapiens (P20594), Homo sapiens (P25092), Homo sapiens (P51841), Mus musculus
Manually annotated by BRENDA team
Usami, S.; Kishimoto, I.; Saito, Y.; Harada, M.; Kuwahara, K.; Nakagawa, Y.; Nakanishi, M.; Yasuno, S.; Kangawa, K.; Nakao, K.
Association of CT dinucleotide repeat polymorphism in the 5-flanking region of the guanylyl cyclase (GC)-A gene with essential hypertension in the Japanese
Hypertens. Res.
31
89-96
2008
Homo sapiens
Manually annotated by BRENDA team
Ellis, D.Z.; Dismuke, W.M.; Chokshi, B.M.
Characterization of soluble guanylate cyclase in NO-induced increases in aqueous humor outflow facility and in the trabecular meshwork
Invest. Ophthalmol. Vis. Sci.
50
1808-1813
2008
Homo sapiens, Sus scrofa
Manually annotated by BRENDA team
Tesmer, J.J.
Guanylyl cyclase sees the light
J. Biol.
7
31
2008
Homo sapiens
Manually annotated by BRENDA team
Sharina, I.G.; Jelen, F.; Bogatenkova, E.P.; Thomas, A.; Martin, E.; Murad, F.
Alpha1 soluble guanylyl cyclase (sGC) splice forms as potential regulators of human sGC activity
J. Biol. Chem.
283
15104-15113
2008
Homo sapiens (Q02108), Homo sapiens
Manually annotated by BRENDA team
Marro, M.L.; Peiro, C.; Panayiotou, C.M.; Baliga, R.S.; Meurer, S.; Schmidt, H.H.; Hobbs, A.J.
Characterization of the human alpha1 beta1 soluble guanylyl cyclase promoter: key role for NF-kappaB(p50) and CCAAT-binding factors in regulating expression of the nitric oxide receptor
J. Biol. Chem.
283
20027-20036
2008
Homo sapiens
Manually annotated by BRENDA team
Peshenko, I.V.; Olshevskaya, E.V.; Dizhoor, A.M.
Binding of guanylyl cyclase activating protein 1 (GCAP1) to retinal guanylyl cyclase (RetGC1). The role of individual EF-hands
J. Biol. Chem.
283
21747-21757
2008
Homo sapiens
Manually annotated by BRENDA team
Frey, R.; Mueck, W.; Unger, S.; Artmeier-Brandt, U.; Weimann, G.; Wensing, G.
Pharmacokinetics, pharmacodynamics, tolerability, and safety of the soluble guanylate cyclase activator cinaciguat (BAY 58-2667) in healthy male volunteers
J. Clin. Pharmacol.
48
1400-1410
2008
Homo sapiens
Manually annotated by BRENDA team
Frey, R.; Mueck, W.; Unger, S.; Artmeier-Brandt, U.; Weimann, G.; Wensing, G.
Single-dose pharmacokinetics, pharmacodynamics, tolerability, and safety of the soluble guanylate cyclase stimulator BAY 63-2521: an ascending-dose study in healthy male volunteers
J. Clin. Pharmacol.
48
926-934
2008
Homo sapiens
Manually annotated by BRENDA team
Lee, H.G.; Kim, S.Y.; Kim, d.u..S.; Seo, S.R.; Lee, S.I.; Shin, D.M.; De Smet, P.; Seo, J.T.
1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one inhibits neurite outgrowth and causes neurite retraction in PC12 cells independently of soluble guanylyl cyclase
J. Neurosci. Res.
87
269-277
2009
Homo sapiens
Manually annotated by BRENDA team
Gambaryan, S.; Kobsar, A.; Hartmann, S.; Birschmann, I.; Kuhlencordt, P.J.; Mueller-Esterl, W.; Lohmann, S.M.; Walter, U.
NO-synthase-/NO-independent regulation of human and murine platelet soluble guanylyl cyclase activity
J. Thromb. Haemost.
6
1376-1384
2008
Homo sapiens, Mus musculus
Manually annotated by BRENDA team
Punathil, T.; Katiyar, S.K.
Inhibition of non-small cell lung cancer cell migration by grape seed proanthocyanidins is mediated through the inhibition of nitric oxide, guanylate cyclase, and ERK1/2
Mol. Carcinog.
48
232-242
2008
Homo sapiens
Manually annotated by BRENDA team
Emmons, T.L.; Mathis, K.J.; Shuck, M.E.; Reitz, B.A.; Curran, D.F.; Walker, M.C.; Leone, J.W.; Day, J.E.; Bienkowski, M.J.; Fischer, H.D.; Tomasselli, A.G.
Purification and characterization of recombinant human soluble guanylate cyclase produced from baculovirus-infected insect cells
Protein Expr. Purif.
65
133-139
2009
Homo sapiens
Manually annotated by BRENDA team
Morbidelli, L.; Pyriochou, A.; Filippi, S.; Vasileiadis, I.; Roussos, C.; Zhou, Z.; Loutrari, H.; Waltenberger, J.; Stoessel, A.; Giannis, A.; Ziche, M.; Papapetropoulos, A.
The soluble guanylyl cyclase inhibitor NS-2028 reduces vascular endothelial growth factor-induced angiogenesis and permeability
Am. J. Physiol. Regul. Integr. Comp. Physiol.
298
R824-R832
2010
Homo sapiens
Manually annotated by BRENDA team
Newton, M.; Niewczas, I.; Clark, J.; Bellamy, T.C.
A real-time fluorescent assay of the purified nitric oxide receptor, guanylyl cyclase
Anal. Biochem.
402
129-136
2010
Homo sapiens
Manually annotated by BRENDA team
Sovershaev, M.A.; Egorina, E.M.; Hansen, J.B.; Osterud, B.; Pacher, P.; Stasch, J.P.; Evgenov, O.V.
Soluble guanylate cyclase agonists inhibit expression and procoagulant activity of tissue factor
Arterioscler. Thromb. Vasc. Biol.
29
1578-1586
2009
Homo sapiens
Manually annotated by BRENDA team
Miller, T.W.; Isenberg, J.S.; Roberts, D.D.
Thrombospondin-1 is an inhibitor of pharmacological activation of soluble guanylate cyclase
Br. J. Pharmacol.
159
1542-1547
2010
Homo sapiens
Manually annotated by BRENDA team
Mueck, W.; Frey, R.
Population pharmacokinetics and pharmacodynamics of cinaciguat, a soluble guanylate cyclase activator, in patients with acute decompensated heart failure
Clin. Pharmacokinet.
49
119-129
2010
Homo sapiens
Manually annotated by BRENDA team
Cardoso, M.H.; Morganti, R.P.; Lilla, S.; Murad, F.; De Nucci, G.; Antunes, E.; Marcondes, S.
The role of superoxide anion in the inhibitory effect of SIN-1 in thrombin-activated human platelet adhesion
Eur. J. Pharmacol.
627
229-234
2010
Homo sapiens
Manually annotated by BRENDA team
Riego, J.A.; Broniowska, K.A.; Kettenhofen, N.J.; Hogg, N.
Activation and inhibition of soluble guanylyl cyclase by S-nitrosocysteine: involvement of amino acid transport system L
Free Radic. Biol. Med.
47
269-274
2009
Homo sapiens
Manually annotated by BRENDA team
Suryanarayana, S.; Goettle, M.; Huebner, M.; Gille, A.; Mou, T.C.; Sprang, S.R.; Richter, M.; Seifert, R.
Differential inhibition of various adenylyl cyclase isoforms and soluble guanylyl cyclase by 2,3-O-(2,4,6-trinitrophenyl)-substituted nucleoside 5-triphosphates
J. Pharmacol. Exp. Ther.
330
687-695
2009
Homo sapiens
Manually annotated by BRENDA team
Ramanathan, S.; Mazzalupo, S.; Boitano, S.; Montfort, W.R.
Thrombospondin-1 and angiotensin II inhibit soluble guanylyl cyclase through an increase in intracellular calcium concentration
Biochemistry
50
7787-7799
2011
Homo sapiens
Manually annotated by BRENDA team
Robinson, J.W.; Potter, L.R.
ATP potentiates competitive inhibition of guanylyl cyclase A and B by the staurosporine analog, Go6976: Reciprocal regulation of ATP and GTP binding
J. Biol. Chem.
286
33841-33844
2011
Homo sapiens
Manually annotated by BRENDA team
Baehre, H.; Danker, K.Y.; Stasch, J.P.; Kaever, V.; Seifert, R.
Nucleotidyl cyclase activity of soluble guanylyl cyclase in intact cells
Biochem. Biophys. Res. Commun.
443
1195-1199
2014
Homo sapiens
Manually annotated by BRENDA team
Seeger, F.; Quintyn, R.; Tanimoto, A.; Williams, G.J.; Tainer, J.A.; Wysocki, V.H.; Garcin, E.D.
Interfacial residues promote an optimal alignment of the catalytic center in human soluble guanylate cyclase: heterodimerization is required but not sufficient for activity
Biochemistry
53
2153-2165
2014
Homo sapiens (Q02108), Homo sapiens
Manually annotated by BRENDA team
Arshad, N.; Ballal, S.; Visweswariah, S.S.
Site-specific N-linked glycosylation of receptor guanylyl cyclase C regulates ligand binding, ligand-mediated activation and interaction with vesicular integral membrane protein 36, VIP36
J. Biol. Chem.
288
3907-3917
2013
Homo sapiens
Manually annotated by BRENDA team
Allerston, C.K.; von Delft, F.; Gileadi, O.
Crystal structures of the catalytic domain of human soluble guanylate cyclase
PLoS ONE
8
e57644
2013
Homo sapiens (Q02108), Homo sapiens
Manually annotated by BRENDA team
Robinson, J.W.; Potter, L.R.
Guanylyl cyclases A and B are asymmetric dimers that are allosterically activated by ATP binding to the catalytic domain
Sci. Signal.
5
ra65
2012
Homo sapiens
Manually annotated by BRENDA team
Soemmer, A.; Sandner, P.; Behrends, S.
BAY 60-2770 activates two isoforms of nitric oxide sensitive guanylyl cyclase Evidence for stable insertion of activator drugs
Biochem. Pharmacol.
147
10-20
2018
Homo sapiens
Manually annotated by BRENDA team
Peshenko, I.V.; Olshevskaya, E.V.; Dizhoor, A.M.
Evaluating the role of retinal membrane guanylyl cyclase 1 (RetGC1) domains in binding guanylyl cyclase-activating proteins (GCAPs)
J. Biol. Chem.
290
6913-6924
2015
Homo sapiens (Q02846)
Manually annotated by BRENDA team
Dickey, D.M.; Edmund, A.B.; Otto, N.M.; Chaffee, T.S.; Robinson, J.W.; Potter, L.R.
Catalytically active guanylyl cyclase B requires endoplasmic reticulum-mediated glycosylation, and mutations that inhibit this process cause dwarfism
J. Biol. Chem.
291
11385-11393
2016
Homo sapiens (P20594)
Manually annotated by BRENDA team
Otto, N.M.; McDowell, W.G.; Dickey, D.M.; Potter, L.R.
A glutamate-substituted mutant mimics the phosphorylated and active form of guanylyl cyclase-A
Mol. Pharmacol.
92
67-74
2017
Homo sapiens (P16066)
Manually annotated by BRENDA team
Agullo, L.; Buch, I.; Gutierrez-de-Teran, H.; Garcia-Dorado, D.; Villa-Freixa, J.
Computational exploration of the binding mode of heme-dependent stimulators into the active catalytic domain of soluble guanylate cyclase
Proteins
84
1534-1548
2016
Homo sapiens (Q02153)
Manually annotated by BRENDA team
Cortese-Krott, M.M.; Mergia, E.; Kramer, C.M.; Lueckstaedt, W.; Yang, J.; Wolff, G.; Panknin, C.; Bracht, T.; Sitek, B.; Pernow, J.; Stasch, J.P.; Feelisch, M.; Koesling, D.; Kelm, M.
Identification of a soluble guanylate cyclase in RBCs preserved activity in patients with coronary artery disease
Redox Biol.
14
328-337
2018
Homo sapiens
Manually annotated by BRENDA team