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Information on EC 4.6.1.17 - cyclic pyranopterin monophosphate synthase and Organism(s) Mycobacterium tuberculosis and UniProt Accession P9WJR7

for references in articles please use BRENDA:EC4.6.1.17
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IUBMB Comments
The enzyme catalyses an early step in the biosynthesis of the molybdenum cofactor (MoCo). In bacteria and plants the reaction is catalysed by MoaC and Cnx3, respectively. In mammals the reaction is catalysed by the MOCS1B domain of the bifuctional MOCS1 protein, which also catalyses EC 4.1.99.22, GTP 3',8-cyclase.
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This record set is specific for:
Mycobacterium tuberculosis
UNIPROT: P9WJR7
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The taxonomic range for the selected organisms is: Mycobacterium tuberculosis
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Synonyms
moac2, molybdenum cofactor biosynthesis protein c, st0472, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Moco-biosynthesis protein
-
molybdenum cofactor biosynthesis protein C
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cnx3
-
-
-
-
MoaC
-
-
-
-
MOCS1B
-
-
-
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
GTP = cyclic pyranopterin phosphate + diphosphate
show the reaction diagram
mechanisms proposed for the first step of the Moco biosynthesis pathway, overview
PATHWAY SOURCE
PATHWAYS
-
-
SYSTEMATIC NAME
IUBMB Comments
(8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate lyase (cyclic pyranopterin phosphate-forming)
The enzyme catalyses an early step in the biosynthesis of the molybdenum cofactor (MoCo). In bacteria and plants the reaction is catalysed by MoaC and Cnx3, respectively. In mammals the reaction is catalysed by the MOCS1B domain of the bifuctional MOCS1 protein, which also catalyses EC 4.1.99.22, GTP 3',8-cyclase.
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
GTP
cyclic pyranopterin phosphate + diphosphate
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
GTP
cyclic pyranopterin phosphate + diphosphate
show the reaction diagram
additional information
?
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
17500
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
2 * 17500, MoaC2 functional form
hexamer
MoaC2 mainly forms hexamers, which are not functional
additional information
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
purified recombinant His-tagged enzyme, hanging drop vapour diffusion method, mixing of 0.003 ml of 10.5 mg/ml protein solution with 0.0015 ml reservoir solution containing 0.025 M potassium sodium tartrate tetrahydrate, pH 8.0, and equilibration against 1 ml reservoir solution, 15 days, X-ray diffraction structure determination and analysis at 3.0 A resolution, molecular replacement calculations
purified recombinant MoaC2s in apo form, X-ray diffraction structure determination and analysis at 2.2-2.5 A resolution
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) to homogeneity by nickel affinity chromatography and gel filtration
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
gene Rv0864, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Srivastava, S.; Srivastava, V.K.; Arora, A.; Pratap, J.V.
Overexpression, purification, crystallization and preliminary X-ray analysis of putative molybdenum cofactor biosynthesis protein C (MoaC2) from Mycobacterium tuberculosis H37Rv
Acta Crystallogr. Sect. F
68
687-691
2012
Mycobacterium tuberculosis (P9WJR7), Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv (P9WJR7), Mycobacterium tuberculosis H37Rv
Manually annotated by BRENDA team
Srivastava, V.K.; Srivastava, S.; Srivastava, S.; Arora, A.; Pratap, J.V.
Structural insights into putative molybdenum cofactor biosynthesis protein C (MoaC2) from Mycobacterium tuberculosis H37Rv
PLoS ONE
8
e58333
2013
Mycobacterium tuberculosis (P9WJR7), Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv (P9WJR7), Mycobacterium tuberculosis H37Rv
Manually annotated by BRENDA team