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Information on EC 4.6.1.16 - tRNA-intron lyase and Organism(s) Sulfurisphaera tokodaii and UniProt Accession Q975R3

for references in articles please use BRENDA:EC4.6.1.16
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EC Tree
IUBMB Comments
The enzyme catalyses the final stage in the maturation of tRNA molecules.
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This record set is specific for:
Sulfurisphaera tokodaii
UNIPROT: Q975R3
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Word Map
The taxonomic range for the selected organisms is: Sulfurisphaera tokodaii
The expected taxonomic range for this enzyme is: Eukaryota, Archaea, Bacteria
Reaction Schemes
pretRNA
=
a 3'-half-tRNA molecule with a 5'-OH end
+
a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end
+
an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus
Synonyms
homing endonuclease, i-tevi, splicing endonuclease, trna splicing endonuclease, hspc117, intron-encoded endonuclease, hclp1, trna-splicing endonuclease, af endonuclease, rna splicing endonuclease, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
splicing endonuclease
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STK_03580
locus name
nuclease, transfer ribonucleate intron endoribo-
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splicing endonuclease
transfer ribonucleate intron endoribonuclease
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transfer splicing endonuclease
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tRNA intron endonuclease
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tRNA splicing endonuclease
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tRNA-intron endonuclease
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-
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tRNATRPintron endonuclease
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-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
endoribonuclease reaction
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-
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PATHWAY SOURCE
PATHWAYS
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-, -
SYSTEMATIC NAME
IUBMB Comments
pretRNA lyase (intron-removing; cyclic-2',3'-phosphate-forming)
The enzyme catalyses the final stage in the maturation of tRNA molecules.
CAS REGISTRY NUMBER
COMMENTARY hide
117444-13-0
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
pretRNA
a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus
show the reaction diagram
-
-
-
?
pre-tRNAArg
?
show the reaction diagram
Q975R3; F9VMU0
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-
-
?
pre-tRNALeu
?
show the reaction diagram
Q975R3; F9VMU0
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-
-
?
pre-tRNATrp
?
show the reaction diagram
Q975R3; F9VMU0
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-
-
?
additional information
?
-
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the heteromeric enzyme complex is composed of two components which are both required for catalytic activity, analysis of reaction products with synthetic tRNA substrate, overview
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-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
pretRNA
a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus
show the reaction diagram
-
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
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heteromeric enzyme complex, both enzyme components are required for catalytic activity
oligomer
Q975R3; F9VMU0
heteromeric enzyme complex is composed of two components which are both required for catalytic activity
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
TALON metal affinity resin column chromatography
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli Rosetta-gami (DE3) cells
expression in Escherichia coli
Q975R3; F9VMU0
two distinct genes endA, ORFs ST0358 and STS047, DNA and amino acid sequence determination and analysis of two ORFs encoding the enzyme, one ORF lacks the putative catalytic amino acid residue, individual expression in Escherichia coli reveals that both are components of an enzyme complex
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Yoshinari, S.; Fujita, S.; Masui, R.; Kuramitsu, S.; Yokobori, S.; Kita, K.; Watanabe, Y.
Functional reconstitution of a crenarchaeal splicing endonuclease in vitro
Biochem. Biophys. Res. Commun.
334
1254-1259
2005
Sulfurisphaera tokodaii, Sulfurisphaera tokodaii 7
Manually annotated by BRENDA team
Yoshinari, S.; Fujita, S.; Masui, R.; Kuramitsu, S.; Yokobori, S.; Kita, K.; Watanabe, Y.
Functional reconstitution of an archaeal splicing endonuclease in vitro
Nucleic Acids Symp. Ser.
49
103-104
2005
Sulfurisphaera tokodaii (Q975R3 and F9VMU0), Sulfurisphaera tokodaii 7 (Q975R3 and F9VMU0)
Manually annotated by BRENDA team
Yamazaki, S.; Yoshinari, S.; Kita, K.; Watanabe, Y.; Kawarabayasi, Y.
Identification of an entire set of tRNA molecules and characterization of cleavage sites of the intron-containing tRNA precursors in acidothermophilic crenarchaeon Sulfolobus tokodaii strain7
Gene
489
103-110
2011
Sulfurisphaera tokodaii (Q975R3), Sulfurisphaera tokodaii, Sulfurisphaera tokodaii 7 (Q975R3)
Manually annotated by BRENDA team