changed residues K20Q, L96C and A131G, vicinal to the active site, are probably responsible for the altered enantioselectivity of LinA-type2 compared to LinA-type1, structural comparison of LinA-type2 and -type1 proteins suggests that thermostability of LinA-type2 might partly arise due to presence of higher number of ionic interactions, along with 4% increase in the intersubunit buried surface area
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quantum mechanics/molecular mechanics study. In the degradation process, two discontinuous dehydrochlorination reactions with the Boltzmann-weighted average potential barriers of 16.2 and 17.3 kcal/mol are connected by a conformational transition with a barrier of 11.1 kcal /mol. Residue Phe68 facilitates the dehydrochlorination of gamma-hexachlorohexane, whereas Leu21 and Cys71 suppress it
gene linA, DNA and amino acid sequence determination and analysis, sequence comparison, subcloning in Escherichia coli strain DH5alpha, expression of C-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3)