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Information on EC 4.4.1.5 - lactoylglutathione lyase and Organism(s) Pseudomonas aeruginosa and UniProt Accession Q9HU72

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EC Tree
IUBMB Comments
Also acts on 3-phosphoglycerol-glutathione.
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This record set is specific for:
Pseudomonas aeruginosa
UNIPROT: Q9HU72
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The taxonomic range for the selected organisms is: Pseudomonas aeruginosa
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Synonyms
glyoxalase i, glyoxalase 1, glo-1, glo-i, glo i, glyoxalase-i, glyoxalase-1, gly-i, lactoylglutathione lyase, glyoxylase i, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
aldoketomutase
-
-
-
-
glyoxalase I
glyoxylase I
-
-
-
-
ketone-aldehyde mutase
-
-
-
-
lyase, lactoylglutathione
-
-
-
-
methylglyoxalase
-
-
-
-
methylglyoxylase
-
-
-
-
S-D-lactoylglutathione methylglyoxal lyase
-
-
-
-
S-D-lactoylglutathione methylglyoxal lyase (isomerizing)
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
PATHWAY SOURCE
PATHWAYS
-
-, -
SYSTEMATIC NAME
IUBMB Comments
(R)-S-lactoylglutathione methylglyoxal-lyase (isomerizing; glutathione-forming)
Also acts on 3-phosphoglycerol-glutathione.
CAS REGISTRY NUMBER
COMMENTARY hide
9033-12-9
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
methylglyoxal + glutathione
(R)-S-lactoylglutathione
show the reaction diagram
first step in the glyoxalase system, detoxification of methylglyoxal
-
-
r
(R)-S-lactoylglutathione
glutathione + methylglyoxal
show the reaction diagram
-
-
-
-
?
glutathione + methylglyoxal
(R)-S-lactoylglutathione
show the reaction diagram
glutathione + methylglyoxal
S-lactoylglutathione
show the reaction diagram
-
-
-
-
?
methylglyoxal + glutathione
(R)-S-lactoylglutathione
show the reaction diagram
first step in the glyoxalase system, detoxification of methylglyoxal
-
-
r
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
methylglyoxal + glutathione
(R)-S-lactoylglutathione
show the reaction diagram
first step in the glyoxalase system, detoxification of methylglyoxal
-
-
r
glutathione + methylglyoxal
(R)-S-lactoylglutathione
show the reaction diagram
-
-
-
-
?
methylglyoxal + glutathione
(R)-S-lactoylglutathione
show the reaction diagram
first step in the glyoxalase system, detoxification of methylglyoxal
-
-
r
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Co2+
activation of gly I
Ni2+
activation of gly I
Zn2+
activation of gloA3, metal ion binds tightly to the enzyme so that removal of metall ion requires more forceful conditions
additional information
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.016 - 0.032
(R)-S-lactoylglutathione
0.016 - 0.032
methylglyoxal
additional information
additional information
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
86 - 271
methylglyoxal
additional information
additional information
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
180
-
presence of Co2+, pH 7.0
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
gloA3
UniProt
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
14250
-
electrospray ionization mass spectrometry
14251
-
x * 14251, electrospray ionization mass spectrometry, x * 14251, calculated
additional information
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
-
x * 14251, electrospray ionization mass spectrometry, x * 14251, calculated
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant enzyme
-
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
isolation and overproduction of glxI enzymes from using Escherichia coli expression systems
expression in Escherichia coli
-
isolation and overproduction of glxI enzymes from Pseudomonas aeruginosa using Escherichia coli expression systems
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Sukdeo, N.; Clugston, S.L.; Daub, E.; Honek, J.F.
Distinct classes of glyoxalase I: metal specificity of the Yersinia pestis, Pseudomonas aeruginosa and Neisseria meningitidis enzymes
Biochem. J.
384
111-117
2004
Neisseria meningitidis, Yersinia pestis, Pseudomonas aeruginosa
Manually annotated by BRENDA team
Sukdeo, N.; Honek, J.F.
Microbial glyoxalase enzymes: metalloenzymes controlling cellular levels of methylglyoxal
Drug Metabol. Drug Interact.
23
29-50
2008
Escherichia coli, Escherichia coli (P0AC81), Homo sapiens, Homo sapiens (Q04760), Leishmania braziliensis, Leishmania donovani, Leishmania major, Leishmania sp., Neisseria meningitidis, Neisseria meningitidis (P0A0T3), Plasmodium falciparum, Pseudomonas aeruginosa, Pseudomonas aeruginosa (Q9HU72), Pseudomonas aeruginosa (Q9HY85), Pseudomonas aeruginosa (Q9I5L8), Pseudomonas putida, Pseudomonas putida (Q88GF8), Trypanosoma cruzi, Yersinia pestis
Manually annotated by BRENDA team
Honek, J.F.
Bacterial glyoxalase I enzymes: structural and biochemical investigations
Biochem. Soc. Trans.
42
479-484
2014
Saccharomyces cerevisiae, Clostridium acetobutylicum, Escherichia coli, Homo sapiens, Leishmania donovani, Leishmania infantum, Leishmania major, Neisseria meningitidis, Yersinia pestis, Plasmodium falciparum, Pseudomonas aeruginosa, Pseudomonas putida, Pseudomonas fluorescens, Pseudomonas syringae, Trypanosoma cruzi
Manually annotated by BRENDA team