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Information on EC 4.4.1.4 - alliin lyase and Organism(s) Citrobacter freundii and UniProt Accession Q84AR1

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EC Tree
     4 Lyases
         4.4 Carbon-sulfur lyases
             4.4.1 Carbon-sulfur lyases (only sub-subclass identified to date)
                4.4.1.4 alliin lyase
IUBMB Comments
A pyridoxal-phosphate protein.
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This record set is specific for:
Citrobacter freundii
UNIPROT: Q84AR1
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The taxonomic range for the selected organisms is: Citrobacter freundii
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Synonyms
alliinase, alliin lyase, cysteine sulfoxide lyase, allinase, alliin-lyase, l-cysteine sulfoxide lyase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
alkylcysteine sulfoxide lyase
-
-
-
-
alliinase
-
-
-
-
cysteine sulfoxide lyase
-
-
-
-
Cysteine sulphoxide lyase
-
-
-
-
L-cysteine sulfoxide lyase
-
-
-
-
lyase, alliin
-
-
-
-
S-alkyl(en)yl-L-cysteine lyase
-
-
-
-
S-alkylcysteine sulfoxide lyase
-
-
-
-
PATHWAY SOURCE
PATHWAYS
-
-, -, -, -, -, -
SYSTEMATIC NAME
IUBMB Comments
S-alkyl-L-cysteine S-oxide alkyl-sulfenate-lyase (2-aminoacrylate-forming)
A pyridoxal-phosphate protein.
CAS REGISTRY NUMBER
COMMENTARY hide
9031-77-0
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(+)-alliin + H2O
allicin + pyruvate + NH3
show the reaction diagram
-
-
-
?
(+)-methiin + H2O
methanesulfenic acid + pyruvate + NH3
show the reaction diagram
-
-
-
?
(+)-propiin + H2O
propane-1-sulfenic acid + pyruvate + NH3
show the reaction diagram
-
-
-
?
(+-)-alliin + H2O
allicin + pyruvate + NH3
show the reaction diagram
-
-
-
?
(+-)-ethiin + H2O
ethanesulfenic acid + pyruvate + NH3
show the reaction diagram
-
-
-
?
(+-)-methiin + H2O
methanesulfenic acid + pyruvate + NH3
show the reaction diagram
-
-
-
?
(+-)-propiin + H2O
propane-1-sulfenic acid + pyruvate + NH3
show the reaction diagram
-
-
-
?
alliin + H2O
allicin + pyruvate + NH3
show the reaction diagram
-
-
-
?
ethiin + H2O
ethanesulfenic acid + pyruvate + NH3
show the reaction diagram
-
-
-
?
methiin + H2O
methanesulfenic acid + pyruvate + NH3
show the reaction diagram
-
-
-
?
S-butyl-L-cysteine + H2O
butanethiol + NH3 + 2-oxopropanoate
show the reaction diagram
-
-
-
?
S-ethyl-L-cysteine + H2O
ethanethiol + NH3 + 2-oxopropanoate
show the reaction diagram
-
-
-
?
S-methyl-L-cysteine + H2O
methanethiol + NH3 + 2-oxopropanoate
show the reaction diagram
-
-
-
?
S-propyl-L-cysteine + H2O
propanethiol + NH3 + 2-oxopropanoate
show the reaction diagram
-
-
-
?
additional information
?
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.2
(+)-alliin
mutant C115H, pH pH 8.0, 30°C
0.54
(+)-methiin
mutant C115H, pH pH 8.0, 30°C
1.4
(+)-propiin
mutant C115H, pH pH 8.0, 30°C
1.26
(+-)-alliin
mutant C115H, pH pH 8.0, 30°C
0.47
(+-)-ethiin
mutant C115H, pH pH 8.0, 30°C
0.57
(+-)-methiin
mutant C115H, pH pH 8.0, 30°C
1.1
(+-)-propiin
mutant C115H, pH pH 8.0, 30°C
0.44 - 1.75
alliin
0.457 - 5.48
ethiin
0.57 - 0.63
methiin
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
13.7
(+)-alliin
mutant C115H, pH pH 8.0, 30°C
5.2
(+)-methiin
mutant C115H, pH pH 8.0, 30°C
21.8
(+)-propiin
mutant C115H, pH pH 8.0, 30°C
12.4
(+-)-alliin
mutant C115H, pH pH 8.0, 30°C
5.59
(+-)-ethiin
mutant C115H, pH pH 8.0, 30°C
5.14
(+-)-methiin
mutant C115H, pH pH 8.0, 30°C
20.2
(+-)-propiin
mutant C115H, pH pH 8.0, 30°C
0.41 - 11.31
alliin
1.25 - 5.59
ethiin
1.63 - 5.14
methiin
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
11
(+)-alliin
mutant C115H, pH pH 8.0, 30°C
9.6
(+)-methiin
mutant C115H, pH pH 8.0, 30°C
16
(+)-propiin
mutant C115H, pH pH 8.0, 30°C
9.8
(+-)-alliin
mutant C115H, pH pH 8.0, 30°C
12
(+-)-ethiin
mutant C115H, pH pH 8.0, 30°C
9
(+-)-methiin
mutant C115H, pH pH 8.0, 30°C
18
(+-)-propiin
mutant C115H, pH pH 8.0, 30°C
0.23 - 25.7
alliin
0.23 - 12
ethiin
2.6 - 9.02
methiin
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
cf. EC 4.4.1.11
SwissProt
Manually annotated by BRENDA team
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
Q84AR1_CITFR
398
0
42915
TrEMBL
-
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
C115A
mutation leads to a decrease of the catalytic efficiency in the gamma-elimination reaction of the physiological substrate by more than an order of magnitude, reation of EC 4.4.1.11
C115H
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Morozova, E.; Kulikova, V.; Rodionov, A.; Revtovich, S.; Anufrieva, N.; Demidkina, T.
Engineered Citrobacter freundii methionine gamma-lyase effectively produces antimicrobial thiosulfinates
Biochimie
128-129
92-98
2016
Citrobacter freundii (Q84AR1)
Manually annotated by BRENDA team
Kulikova, V.; Morozova, E.; Rodionov, A.; Koval, V.; Anufrieva, N.; Revtovich, S.; Demidkina, T.
Non-stereoselective decomposition of (+-)-S-alk(en)yl-l-cysteine sulfoxides to antibacterial thiosulfinates catalyzed by C115H mutant methionine gamma-lyase from Citrobacter freundii
Biochimie
151
42-44
2018
Citrobacter freundii (Q84AR1), Citrobacter freundii
Manually annotated by BRENDA team