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Information on EC 4.4.1.35 - L-cystine beta-lyase and Organism(s) Arabidopsis thaliana and UniProt Accession Q9SUR6

for references in articles please use BRENDA:EC4.4.1.35
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EC Tree
IUBMB Comments
A pyridoxal 5'-phosphate protein. The enzyme cleaves a carbon-sulfur bond, releasing L-thiocysteine and an unstable enamine product that tautomerizes to an imine form, which undergoes a hydrolytic deamination to form pyruvate and ammonia. The latter reaction, which can occur spontaneously, can also be catalysed by EC 3.5.99.10, 2-iminobutanoate/2-iminopropanoate deaminase. The enzyme from Brassica oleracea var. italica (broccoli) does not act on cysteine or cystathionine.
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Arabidopsis thaliana
UNIPROT: Q9SUR6
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Word Map
The taxonomic range for the selected organisms is: Arabidopsis thaliana
The enzyme appears in selected viruses and cellular organisms
Reaction Schemes
hide(Overall reactions are displayed. Show all >>)
Synonyms
cori3, bocl3, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
cis-3-hydroxy-L-proline hydro-lyase (1-pyrroline-2-carboxylate-forming)
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CORI3
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PATHWAY SOURCE
PATHWAYS
SYSTEMATIC NAME
IUBMB Comments
L-cystine thiocysteine-lyase (deaminating; pyruvate-forming)
A pyridoxal 5'-phosphate protein. The enzyme cleaves a carbon-sulfur bond, releasing L-thiocysteine and an unstable enamine product that tautomerizes to an imine form, which undergoes a hydrolytic deamination to form pyruvate and ammonia. The latter reaction, which can occur spontaneously, can also be catalysed by EC 3.5.99.10, 2-iminobutanoate/2-iminopropanoate deaminase. The enzyme from Brassica oleracea var. italica (broccoli) does not act on cysteine or cystathionine.
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
L-cystine + H2O
L-thiocysteine + pyruvate + NH3
show the reaction diagram
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?
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2.32
L-cystine
pH 7.6, 22°C
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
CORI3_ARATH
422
0
47039
Swiss-Prot
other Location (Reliability: 4)
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Jones, P.R.; Manabe, T.; Awazuhara, M.; Saito, K.
A new member of plant CS-lyases. A cystine lyase from Arabidopsis thaliana
J. Biol. Chem.
278
10291-10296
2003
Arabidopsis thaliana (Q9SUR6), Arabidopsis thaliana, Brassica oleracea (Q84UD0), Brassica oleracea
Manually annotated by BRENDA team