Information on EC 4.4.1.24 - (2R)-sulfolactate sulfo-lyase

for references in articles please use BRENDA:EC4.4.1.24
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:

The enzyme appears in viruses and cellular organisms

top print hide
EC NUMBER
COMMENTARY hide
4.4.1.24
-
top print hide
RECOMMENDED NAME
GeneOntology No.
(2R)-sulfolactate sulfo-lyase
top print hide
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
(2R)-3-sulfolactate = pyruvate + HSO3-
show the reaction diagram
top print hide
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
(R)-cysteate degradation
-
-
sulfolactate degradation I
-
-
Cysteine and methionine metabolism
-
-
top print hide
SYSTEMATIC NAME
IUBMB Comments
(2R)-3-sulfolactate bisulfite-lyase (pyruvate-forming)
Requires iron(II). This inducible enzyme participates in cysteate degradation by the bacterium Paracoccus pantotrophus NKNCYSA and in 3-sulfolactate degradation by the bacterium Chromohalobacter salexigens. The enzyme is specific for the (R) isomer of its substrate.
top
top print hide
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strongly inducible enzyme, gene suyAB
SwissProt
Manually annotated by BRENDA team
top print hide
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(2R)-3-sulfolactate
pyruvate + bisulfite
show the reaction diagram
3-sulfolactate
pyruvate + bisulfite
show the reaction diagram
additional information
?
-
activity with 3-sulfopyruvate in vivo by cysteate-grown cells only after membrane permeabilization
-
-
-
top print hide
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
3-sulfolactate
pyruvate + bisulfite
show the reaction diagram
Q58Y44
desulfonation step involved in cysteate dissimilation, metabolic pathway in the cell, overview
-
-
?
additional information
?
-
Q58Y44
activity with 3-sulfopyruvate in vivo by cysteate-grown cells only after membrane permeabilization
-
-
-
top print hide
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Co2+
can substitute for Fe2+ at 2mM
Fe2+
required for activity
Mn2+
can substitute for Fe2+ at 2mM
top print hide
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
EDTA
70% inhibition at 1 mM
top print hide
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
-
top print hide
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.016
-
crude extract
0.13
-
crude extract
0.2
-
in the soluble fraction
0.3
purified enzyme
top print hide
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
i.e. 2-amino-3-sulfopropionate, is the sole carbon and energy source of the organism with either nitrate or molecular oxygen as terminal electron acceptor
Manually annotated by BRENDA team
top print hide
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
top print hide
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8000
x * 8000, alpha-subunit, + x * 42000, beta-subunit, SDS-PAGE
top print hide
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 8000, alpha-subunit, + x * 42000, beta-subunit, SDS-PAGE
top print hide
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
after freezing no residual enzyme activity
-
top print hide
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
4�C, 1 day, 50% loss of activity
-
top print hide
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
from cysteate-grown cells by anion exchange chromatography and hydrophobic interaction chromatography, to near homogeneity
top print hide
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
gene suyAB, DNA and amino acid sequence determination and analysis
top
Show AA Sequence (126 entries)
Please use the Sequence Search for a specific query.
top
top
LINKS TO OTHER DATABASES (specific for EC-Number 4.4.1.24)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
InterPro (database of protein families, domains and functional sites)