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Information on EC 4.4.1.15 - D-cysteine desulfhydrase and Organism(s) Arabidopsis thaliana and UniProt Accession F4HYF3

for references in articles please use BRENDA:EC4.4.1.15
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This record set is specific for:
Arabidopsis thaliana
UNIPROT: F4HYF3 not found.
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Word Map
The taxonomic range for the selected organisms is: Arabidopsis thaliana
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Reaction Schemes
Synonyms
d-cysteine desulfhydrase, d-cdes, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D-cysteine desulfhydrase
-
-
D-cysteine lyase
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
C-S-bond cleavage
-
-
-
-
elimination of H2S or RSH
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
D-cysteine sulfide-lyase (deaminating; pyruvate-forming)
-
CAS REGISTRY NUMBER
COMMENTARY hide
84012-74-8
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
D-cysteine + H2O
sulfide + NH3 + pyruvate
show the reaction diagram
L-cysteine + H2O
sulfide + NH3 + pyruvate
show the reaction diagram
-
5% activity compared to D-cysteine
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
D-cysteine + H2O
sulfide + NH3 + pyruvate
show the reaction diagram
-
-
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
pyridoxal 5'-phosphate
-
pyridoxal 5'-phosphate
-
-
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
aminooxy acetic acid
-
at 10 microM, IC50 value is 0.0305 mM
hydroxylamine
-
at 10 microM, IC50 value is 0.0159 mM
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.25
D-cysteine
-
purified recombinant mature protein
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
6
D-cysteine
-
-
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0305
aminooxy acetic acid
Arabidopsis thaliana
-
at 10 microM, IC50 value is 0.0305 mM
0.0159
hydroxylamine
Arabidopsis thaliana
-
at 10 microM, IC50 value is 0.0159 mM
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
loss of activity at 50°C, no activity at 60°C. The enzyme is also very sensitive to freezing. One freeze-thaw cycle leads to a loss of activity of 75%.
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.34
-
mature protein without N-terminal extension, calculated from amino acid sequence
7.2
-
calculated from amino acid sequence
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
high expression
Manually annotated by BRENDA team
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
the two cysteine desulfhydrases, L-cysteine desulfhydrase and D-cysteine desulfhydrase, are mainly responsible for the degradation of cysteine in order to generate H2S, they show similar expression patterns in tissues
physiological function
D-cysteine desulfhydrase is one of two enzymes mainly responsible for the degradation of cysteine in order to generate H2S, the most important is L-cysteine desulfhydrase, EC 4.4.1.1. Gene expression regulation relationship to drought tolerance in Arabidopsis thaliana, protective effect of H2S against drought, and H2S induces stomatal closure, overview
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
DCYD1_ARATH
401
0
43900
Swiss-Prot
Mitochondrion (Reliability: 5)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
41700
-
monomer, mature protein without N-terminal extension, calculated from amino acid sequence
42000
-
x * 42000, SDS-PAGE, unprocessed protein
43000
-
x * 43000, SDS-PAGE, mature protein
43900
-
monomer, calculated from amino acid sequence
44000
-
x * 44000, SDS-PAGE, post-translationally modified protein
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
glycerol, pyridoxal-5'-phosphate, dithiothreitol and EDTA do not increase the stability of the protein after freezing
-
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant protein
-
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
semi-quantitative reverse transcription-PCR analysis
expression in Escherichia coli
-
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
H2S fumigation stimulates the expression of drought associated genes. The enzyme is strongly induced by drought stress, with a maximum accumulation after 6 h, overview
the enzyme expression levels gradually increases in an age-dependent manner
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Riemenschneider, A.; Wegele, R.; Schmidt, A.; Papenbrock, J.
Isolation and characterization of a D-cysteine desulfhydrase protein from Arabidopsis thaliana
FEBS J.
272
1291-1304
2005
Arabidopsis thaliana
Manually annotated by BRENDA team
Jin, Z.; Shen, J.; Qiao, Z.; Yang, G.; Wang, R.; Pei, Y.
Hydrogen sulfide improves drought resistance in Arabidopsis thaliana
Biochem. Biophys. Res. Commun.
414
481-486
2011
Arabidopsis thaliana (F4HYF3), Arabidopsis thaliana, Arabidopsis thaliana Col-0 (F4HYF3)
Manually annotated by BRENDA team