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Information on EC 4.4.1.11 - methionine gamma-lyase and Organism(s) Treponema denticola and UniProt Accession Q73KL7

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EC Tree
IUBMB Comments
A pyridoxal-phosphate protein. The enzyme cleaves a carbon-sulfur bond, releasing methanethiol and an unstable enamine product that tautomerizes to an imine form, which undergoes a hydrolytic deamination to form 2-oxobutanoate and ammonia. The latter reaction, which can occur spontaneously, can also be catalysed by EC 3.5.99.10, 2-iminobutanoate/2-iminopropanoate deaminase. The enzyme is involved in L-methionine catabolism.
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Treponema denticola
UNIPROT: Q73KL7
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The taxonomic range for the selected organisms is: Treponema denticola
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Synonyms
mgl, rmetase, metase, methioninase, l-methioninase, methionine gamma-lyase, l-methionine gamma-lyase, methionine-gamma-lyase, cale6, l-methionase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
L-methioninase
L-methionine gamma-lyase
-
-
-
-
L-methionine-alpha-deamino-gamma-mercaptomethane lyase
-
-
L-methionine-alpha-deamino-gamma-mercaptomethane-lyase
-
-
-
-
L-methionine-gamma-lyase
-
-
lyase, methionine
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-
-
-
methioninase
methionine dethiomethylase
-
-
-
-
methionine gamma-lyase
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-
-
-
methionine lyase
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-
-
-
SYSTEMATIC NAME
IUBMB Comments
L-methionine methanethiol-lyase (deaminating; 2-oxobutanoate-forming)
A pyridoxal-phosphate protein. The enzyme cleaves a carbon-sulfur bond, releasing methanethiol and an unstable enamine product that tautomerizes to an imine form, which undergoes a hydrolytic deamination to form 2-oxobutanoate and ammonia. The latter reaction, which can occur spontaneously, can also be catalysed by EC 3.5.99.10, 2-iminobutanoate/2-iminopropanoate deaminase. The enzyme is involved in L-methionine catabolism.
CAS REGISTRY NUMBER
COMMENTARY hide
42616-25-1
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
L-cysteine + H2O
sulfide + NH3 + pyruvate
show the reaction diagram
-
-
-
?
L-methionine + H2O
methanethiol + NH3 + 2-oxobutanoate
show the reaction diagram
-
-
-
?
L-homocysteine + H2O
hydrogen sulfide + NH3 + 2-oxobutanoate
show the reaction diagram
-
-
-
-
?
L-methionine + H2O
?
show the reaction diagram
-
-
-
-
?
L-methionine + H2O
methanethiol + NH3 + 2-oxobutanoate
show the reaction diagram
-
-
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
L-methionine + H2O
methanethiol + NH3 + 2-oxobutanoate
show the reaction diagram
-
-
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
pyridoxal 5'-phosphate
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-
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
aminoethyloxyvinylglycine
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-
myrsinoic acid B
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propargylglycine
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-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.18
L-cysteine
pH 7.6, 37°C
0.55
L-methionine
-
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.79
L-cysteine
pH 7.6, 37°C
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
4.46
L-cysteine
pH 7.6, 37°C
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0303
myrsinoic acid B
Treponema denticola
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in 50 mM Tris-HCl (pH 7.5) containing 0.05 mM pyridoxal 5'-phosphate, at 37°C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
value is 36.6 U/mg protein
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
UniProt
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
167500
gel filtration
43500
4 * 43500, calculated and SDS-PAGE
43500
-
SDS-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
tetramer
4 * 43500, calculated and SDS-PAGE
tetramer
-
4*43500, SDS-PAGE
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
hanging-drop vapor-diffusion method
-
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
ion exchange and gel filtration chromatography
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
-
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
drug development
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methyl mercaptan may not only cause oral malodour but also play an important role in the pathogenicity of Treptonema denticola. The enzyme may be an exploitable target for novel chemotherapeutic drugs. An inhibitor of METase may represent a new class of compounds with the potential for preventing and treating oral malodor
medicine
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enzyme therapy against various types of methionine-dependent tumors
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Fukamachi, H.; Nakano, Y.; Okano, S.; Shibata, Y.; Abiko, Y.; Yamashita, Y.
High production of methyl mercaptan by L-methionine-alpha-deamino-gamma-mercaptomethane lyase from Treponema denticola
Biochem. Biophys. Res. Commun.
331
127-131
2005
Porphyromonas gingivalis, Treponema denticola
Manually annotated by BRENDA team
Ito, S.; Narise, A.; Shimura, S.
Identification of a methioninase inhibitor, myrsinoic acid B, from Myrsine seguinii Lev., and its inhibitory activities
Biosci. Biotechnol. Biochem.
72
2411-2414
2008
Fusobacterium nucleatum, Fusobacterium nucleatum JCM 8532, Porphyromonas gingivalis, Porphyromonas gingivalis W83, Treponema denticola, Treponema denticola ATCC 35405
Manually annotated by BRENDA team
El-Sayed, A.S.
Microbial L-methioninase: production, molecular characterization, and therapeutic applications
Appl. Microbiol. Biotechnol.
86
445-467
2010
Achromobacter starkeyi, Aeromonas sp., Aspergillus flavipes, Aspergillus sp., Citrobacter freundii, Citrobacter intermedius, Cladosporium cladosporioides, Clostridium sporogenes, Entamoeba histolytica, Lactococcus lactis, no activity in mammalia, Pseudomonas putida, Treponema denticola, Trichomonas vaginalis, Brevibacterium linens BL2, Aspergillus sp. Rs-1a
Manually annotated by BRENDA team
Suwabe, K.; Yoshida, Y.; Nagano, K.; Yoshimura, F.
Identification of an L-methionine gamma-lyase involved in the production of hydrogen sulfide from L-cysteine in Fusobacterium nucleatum subsp. nucleatum ATCC 25586
Microbiology
157
2992-3000
2011
Treponema denticola (Q73KL7), Porphyromonas gingivalis (Q7MX71), Fusobacterium nucleatum subsp. nucleatum (Q8RDT4), Porphyromonas gingivalis W83 (Q7MX71), Treponema denticola ATCC 35405 (Q73KL7), Fusobacterium nucleatum subsp. nucleatum ATCC 25586 / JCM14847 (Q8RDT4)
Manually annotated by BRENDA team