Information on EC 4.3.2.6 - gamma-L-glutamyl-butirosin B gamma-glutamyl cyclotransferase

for references in articles please use BRENDA:EC4.3.2.6
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The expected taxonomic range for this enzyme is: Bacillus circulans

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EC NUMBER
COMMENTARY hide
4.3.2.6
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RECOMMENDED NAME
GeneOntology No.
gamma-L-glutamyl-butirosin B gamma-glutamyl cyclotransferase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
gamma-L-glutamyl-butirosin B = butirosin B + 5-oxoproline
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
butirosin biosynthesis
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Neomycin, kanamycin and gentamicin biosynthesis
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Biosynthesis of antibiotics
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SYSTEMATIC NAME
IUBMB Comments
gamma-L-glutamyl-butirosin B gamma-glutamyl cyclotransferase (5-oxoproline producing)
The enzyme catalyses the last step in the biosynthesis of the aminoglycoside antibiotic butirosin B. The enzyme acts as a cyclotransferase, cleaving the amide bond via transamidation using the alpha-amine of the terminal gamma-L-glutamate of the side chain, releasing it as the cyclic 5-oxo-L-proline.
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
gamma-L-glutamyl-butirosin B
butirosin B + 5-oxoproline
show the reaction diagram
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
18100
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1 * 18100, calculated from amino acid sequence
20093
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1 * 20093, His-tagged enzyme, LC-ESI mass spectrometry
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SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Ni2+-NTA affinity column chromatography
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3) cells
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LINKS TO OTHER DATABASES (specific for EC-Number 4.3.2.6)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
InterPro (database of protein families, domains and functional sites)