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IUBMB Comments The enzyme, from the bacterium Salmonella typhimurium, is involved in the degradation pathway of 2-amino-2-deoxy-D-gluconate.
The enzyme appears in viruses and cellular organisms
Synonyms
d-glucosaminate-6-phosphate ammonia lyase,
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D-glucosaminate-6-phosphate ammonia lyase
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D-glucosaminic acid 6-phosphate dehydratase
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DgaE
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2-amino-2-deoxy-D-gluconate 6-phosphate = 2-dehydro-3-deoxy-6-phospho-D-gluconate + NH3
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D-glucosaminate-6-phosphate ammonia-lyase (2-dehydro-3-deoxy-6-phospho-D-gluconate-forming)
The enzyme, from the bacterium Salmonella typhimurium, is involved in the degradation pathway of 2-amino-2-deoxy-D-gluconate.
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2-amino-2-deoxy-D-gluconate 6-phosphate
2-dehydro-3-deoxy-6-phospho-D-gluconate + NH3
6-phospho-D-glucosaminate
2-dehydro-3-deoxy-6-phospho-D-gluconate + NH3
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2-amino-2-deoxy-D-gluconate 6-phosphate
2-dehydro-3-deoxy-6-phospho-D-gluconate + NH3
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2-amino-2-deoxy-D-gluconate 6-phosphate
2-dehydro-3-deoxy-6-phospho-D-gluconate + NH3
the enzyme is highly efficient and highly specific for 2-amino-2-deoxy-D-gluconate 6-phosphate. A mechanism is proposed that requires a second catalytic base located on the D-face of the substrate-pyridoxal-5'-phosphate complex. Deprotonation is followed by elimination of water to give an aminoacrylate intermediate, which tautomerizes and undergoes hydrolysis to give the product, 2-dehydro-3-deoxygluconate-6-phosphate
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2-amino-2-deoxy-D-gluconate 6-phosphate
2-dehydro-3-deoxy-6-phospho-D-gluconate + NH3
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6-phospho-D-glucosaminate
2-dehydro-3-deoxy-6-phospho-D-gluconate + NH3
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pyridoxal 5'-phosphate
a second catalytic base located on the D-face of the substrate-pyridoxal 5'-phosphate complex. Deprotonation is followed by elimination of water to give an aminoacrylate intermediate, which tautomerizes and undergoes hydrolysis to give the product, 2-dehydro-3-deoxygluconate-6-phosphate
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KCl
modest (about 30%) activation at 0.05 M, strongly inhibition (more than 50%) at higher concentrations (0.5 M)
NaCl
modest (about 30%) activation at 0.05 M, strongly inhibition (more than 50%) at higher concentrations (0.5 M)
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alpha-glycerophosphate
weak competitive inhibitor
phosphate
weak competitive inhibitor
additional information
no significant inhibition with D-serine at concentrations up to 200 mM
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additional information
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no significant inhibition with D-serine at concentrations up to 200 mM
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0.14
2-amino-2-deoxy-D-gluconate 6-phosphate
pH 8, 37°C
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27.4
2-amino-2-deoxy-D-gluconate 6-phosphate
pH 8, 37°C
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196
2-amino-2-deoxy-D-gluconate 6-phosphate
pH 8, 37°C
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32
alpha-glycerophosphate
pH 8, 37°C
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6.2
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in 0.1 M potassium phosphate, pH 7.0, at 37°C
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serovar typhimurium
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brenda
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SwissProt
brenda
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brenda
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DGAE_SALT1
Salmonella typhimurium (strain 14028s / SGSC 2262)
369
0
39323
Swiss-Prot
A0A345EQJ3_ECOLX
369
0
39451
TrEMBL
T0QPT0_AERSA
371
0
39687
TrEMBL
A0A2Z4AFL0_9BACT
372
0
40698
TrEMBL
A0A508YA58_LACPA
366
0
39704
TrEMBL
A0A369QHM0_9BACT
561
0
60648
TrEMBL
A0A2Z4AAZ9_9BACT
398
0
42683
TrEMBL
A0A4P7KYZ8_9GAMM
371
0
40342
TrEMBL
A0A2H5YPB1_9BACT
402
0
42468
TrEMBL
A0A563XAY2_ENTFL
368
0
40098
TrEMBL
A0A2S6SFK2_9PROT
402
0
45558
TrEMBL
A0A1C6Z2K9_HAFAL
374
0
40436
TrEMBL
A0A395L6N1_9RHIZ
399
0
42970
TrEMBL
A0A2C1A9P7_BACCE
367
0
40184
TrEMBL
A0A486V487_KLEPN
372
0
40100
TrEMBL
A0A563WXC7_ENTFL
126
0
14147
TrEMBL
A0A2S6SSF7_9PROT
408
0
46479
TrEMBL
A0A563WGT4_ENTFL
368
0
40180
TrEMBL
A0A3P4I8B3_KLEPN
372
0
40025
TrEMBL
A0A383R9F6_PAEAL
370
0
40052
TrEMBL
A0A563X1I5_ENTFL
367
0
40097
TrEMBL
A0A563XHX0_ENTFL
318
0
34580
TrEMBL
A0A2H5YRD5_9BACT
383
0
41489
TrEMBL
A0A221UTY9_9FLAO
404
1
43936
TrEMBL
A0A4P6LSU9_9FIRM
365
0
40334
TrEMBL
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dimer
2 * 39323, calculated from sequence
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Ni affinity column chromatography
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expressed in Escherichia coli BL21(DE3lambda) cells
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Miller, K.A.; Phillips, R.S.; Mrazek, J.; Hoover, T.R.
Salmonella utilizes D-glucosaminate via a mannose family phosphotransferase system permease and associated enzymes
J. Bacteriol.
195
4057-4066
2013
Salmonella enterica subsp. enterica
brenda
Phillips, R.S.; Ting, S.C.; Tetsadjio, A.G.; Anderson, K.L.; Friez, K.M.; Miller, K.A.; Hoover, T.R.
Properties and mechanism of D-glucosaminate-6-phosphate ammonia-lyase An aminotransferase family enzyme with D-amino acid specificity
Biochim. Biophys. Acta
1866
799-805
2018
Salmonella enterica subsp. enterica serovar Typhimurium (D0ZLR3), Salmonella enterica subsp. enterica serovar Typhimurium
brenda
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Transporter Classification Database (TCDB):
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