Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
5'-CTCTCCCTTC-5,6-dihydrouracil-CTCCTTTCCTCT-3'
?
-
-
-
?
5'-CTCTCCCTTC-8-oxo-7,8-dihydroguanine-CTCCTTTCCTCT-3'
?
-
-
-
?
5'-GACAAGCGCAG-(5R,6S)-2'-deoxy-5,6-dihydroxyuridine-CAGCCGAACAC-3'
?
-
-
-
?
5'-GACAAGCGCAG-(5S,6R)-2'-deoxy-5,6-dihydroxyuridine-CAGCCGAACAC-3'
?
-
-
-
?
DNA containing apurinic/apyrimidinic sites
?
-
-
-
?
35 base pair oligonucleotide containing 5,6-dihydrouracil opposite A
?
-
-
-
-
?
35 base pair oligonucleotide containing 5,6-dihydrouracil opposite G
?
-
-
-
-
?
35 base pair oligonucleotide containing 5,6-dihydroxy-5,6-dihydrothymine opposite A
?
-
-
-
-
?
35 base pair oligonucleotide containing 5,6-dihydroxy-5,6-dihydrothymine opposite G
?
-
-
-
-
?
AP-DNA
fragments of DNA
-
-
-
-
?
DNA containing 2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine/C
?
-
-
-
-
?
DNA containing 5,6-dihydrothymidine/A
?
-
-
-
-
?
DNA containing 5-hydroxy-2'-deoxyuridine/G
?
-
-
-
-
?
DNA containing apurinic sites
?
-
-
-
-
?
DNA containing apurinic/apyrimidinic sites
fragments of DNA
DNA containing O-benzylhydroxylamine
?
-
-
-
-
?
DNA containing O-methylhydroxylamine
?
-
-
-
-
?
DNA containing tetrahydrofuranyl/G
?
-
-
-
-
?
DNA containing thymine glycol
?
-
-
-
-
?
DNA containing urea
?
-
-
-
-
?
DNA with 2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine/C
?
-
-
-
-
?
DNA with 5,6-dihydrothymidine/A
?
-
-
-
-
?
DNA with 5,6-dihydrothymine
?
-
-
-
-
?
DNA with 5,6-dihydrouracil
?
-
-
-
-
?
DNA with 5-hydroxy-2'-deoxyuridine/G
?
-
-
-
-
?
DNA with 5-hydroxy-5-methylhydantoin
?
-
-
-
-
?
DNA with 5-hydroxy-6-hydrothymine
?
-
-
-
-
?
DNA with 5-hydroxy-6-hydrouracil
?
-
-
-
-
?
DNA with alloxan
?
-
-
-
-
?
DNA with thymine glycol
?
-
-
-
-
?
DNA with uracil glycol
?
-
-
-
-
?
additional information
?
-
DNA
fragments of DNA
-
-
-
-
?
DNA
fragments of DNA
-
-
-
?
DNA
fragments of DNA
-
-
-
-
?
DNA
fragments of DNA
-
-
-
?
DNA
fragments of DNA
-
-
-
?
DNA
fragments of DNA
-
-
-
-
?
DNA
fragments of DNA
-
-
-
?
DNA
fragments of DNA
-
-
-
-
?
DNA
fragments of DNA
-
-
-
?
DNA
fragments of DNA
-
-
-
-
?
DNA
fragments of DNA
-
-
-
?
DNA
fragments of DNA
-
-
-
-
?
DNA
fragments of DNA
-
cleavage at abasic sites in duplexes with paired lesions is slower than in duplexes with single lesions. Double strand breaks are readily generated in duplexes with abasic sites positioned 3' to each other. In duplexes containing abasic sites set 1 base pair apart, 5' to each other, both enzymes slowly cleave the abasic site on one strand only and are unable to incise the other stand
-
?
DNA
fragments of DNA
-
removes 5-hydroxy-2'-deoxycytidine and 5-hydroxy-2'-deoxyuridine via a N-glycosylase/beta-elimination reaction
-
?
DNA
fragments of DNA
-
cuts the DNA strands on the 5' side of the apurinic sites giving a 3'-OH and a 5'-phosphate
-
?
DNA
fragments of DNA
-
endonucleolytic cleavage near apurinic or apyrimidinic sites to products with 5'-phosphates, e.g. UV-irradiated poly(dA)*poly(dT)
-
?
DNA
fragments of DNA
-
catalyzes phosphodiester bond cleavage via a lyase- rather than a hydrolase mechanism
-
?
DNA
fragments of DNA
-
synthetic oligodeoxynucleotides containing abasic sites
-
?
DNA
fragments of DNA
-
specific for apurinic sites
-
?
DNA
fragments of DNA
-
endonuclease III: excision of a number of thymine- and cytosine-derived lesions from DNA, no purine-derived lesions excised by endonuclease III
-
?
DNA
fragments of DNA
-
endonucleolytic activity against apurinic and apyrimidinic sites and a dose-dependent response to DNA that has been X-irradiated, UV-irradiated or treated with OsO4
-
?
DNA
fragments of DNA
-
cleaves the phosphodiester bond 3' to the apurinic/apyrimidinic site
-
?
DNA
fragments of DNA
-
cleaves the phosphodiester bond 3' to the apurinic/apyrimidinic site
-
?
DNA
fragments of DNA
-
cleaves the phosphodiester bond 3' to the apurinic/apyrimidinic site
-
?
DNA
fragments of DNA
-
duplex oligonucleotides containing the base lesion analogs O-methylhydoxylamine or O-benzylhydroxylamine, N-glycosylase activity generates intermediary AP site which is subsequently cleaved by the enzyme -associated AP lyase activity, O-alkoxyamine-modified AP sites are poorer substrates than the presumed physiological substrates
-
?
DNA
fragments of DNA
-
enzyme generates only single-strand breaks when the base damage is set one and three base-pairs apart, and only slowly introduces double-strand breaks in substrates where base damage is set five or seven base-pairs apart. Treatment of an abasic site-containing DNA readily yields double-strand breaks.
-
?
DNA
fragments of DNA
-
DNA containing dihydrouridine, 5,3-dihydrothymidine, 5-hydroxy-2'-deoxyuridine, 2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine or tetrahydrofuranyl
-
?
DNA
fragments of DNA
-
the beta-elimination reaction breaking the C3'-O-P bond 3' to an AP site can be followed by a delta-elimination reaction breaking the C5'-O-P bond 5' to the AP site, with the release of an unsaturated derivative of the base-free sugar and the generation of a gap flanked by 3'-phosphate and 5'-phosphate ends
-
?
DNA
fragments of DNA
-
recognizes urea, an oxidative ring fragmentation product of thymine
-
?
DNA
fragments of DNA
-
when DNA containing thymine glycol is used as substrate the combined N-glycosylase/AP endonuclease activity is about 2fold higher than the AP endonuclease activity
-
?
DNA
fragments of DNA
-
KsgA has al DNA glycosylase/AP lyase activity for C mispaired with oxidized T
-
-
?
DNA containing apurinic/apyrimidinic sites
fragments of DNA
-
specificity
-
-
?
DNA containing apurinic/apyrimidinic sites
fragments of DNA
-
C4'-oxidized abasic sites are efficiently excised via intermediate Schiff-base formation. Activity is 100fold less efficient than repair by exonuclease III
-
-
?
DNA containing apurinic/apyrimidinic sites
fragments of DNA
-
endonuclease III plays an important cellular role by removing premutagenic pyrimidine damages produced by reactive oxygen species. EcoNth is a bifunctional enzyme that has DNA glycosylase and apurinic/apyrimidinic lyase activity
-
-
?
DNA containing apurinic/apyrimidinic sites
fragments of DNA
-
the enzyme forms a Schiff base-type intermediate with the substrate after the damaged base is removed
-
-
?
additional information
?
-
-
enzyme also has DNA N-glycosylase activity
-
-
?
additional information
?
-
-
enzyme also has DNA N-glycosylase activity
-
-
?
additional information
?
-
-
enzyme also has DNA N-glycosylase activity
-
-
?
additional information
?
-
-
enzyme also has DNA N-glycosylase activity
-
-
?
additional information
?
-
-
all known bacterial AP lyases and some at least of the mammalian ones, also acts as DNA glycosylases
-
-
?
additional information
?
-
-
bacteriophage T4 endonuclease V and Escherichia coli endonuclease II catalyze N-glycosylase and 3'-abasic endonuclease reaction, beta-elimination reaction
-
-
?
additional information
?
-
-
no substrate: alkylated sites
-
-
?
additional information
?
-
-
no substrate: native DNA
-
-
?
additional information
?
-
-
pdT8d(-)dTn is cleaved by endonuclease III yielding two products which have the same electrophoretic behaviour as the doublet obtained by alkaline beta-elimination, thus the enzyme is a beta-elimination catalyst
-
-
?
additional information
?
-
-
KsgA does not remove C opposite normal bases, 7,8-dihydro-8-oxoguanine and 2-hydroxyadenine, KsgA does not excise thymine glycol, 5-formyluracil, and 5-hydroxymethyluracil opposite cytosine from double-stranded oligonucleotides
-
-
?
additional information
?
-
Escherichia coli endonuclease III is a DNA glycosylase with a broad substrate specificity for oxidized or reduced pyrimidine bases. Endo III possesses two types of activities: N-glycosylase (hydrolysis of the N-glycosidic bond) and AP lyase (elimination of the 3'-phosphate of the AP-site)
-
-
?
additional information
?
-
-
Escherichia coli endonuclease III is a DNA glycosylase with a broad substrate specificity for oxidized or reduced pyrimidine bases. Endo III possesses two types of activities: N-glycosylase (hydrolysis of the N-glycosidic bond) and AP lyase (elimination of the 3'-phosphate of the AP-site)
-
-
?
additional information
?
-
analysis of structural rearrangements of the DNA substrates and uncleavable ligands during their interaction with Endo III using oligonucleotide duplexes containing 5,6-dihydrouracil, a natural abasic site, its tetrahydrofuran analogue, and undamaged duplexes carried fluorescent DNA base analogues 2-aminopurine and 1,3-diaza-2-oxophenoxazine as environment-sensitive reporter groups, pre-steady-state kinetic analysis, overview. Endo III induces several fast sequential conformational changes in DNA during binding, lesion recognition, and adjustment to a catalytically competent conformation. The glycosylase uses a multistep mechanism of damage recognition, which likely involves Gln41 and Leu81 as DNA lesion sensors
-
-
?
additional information
?
-
-
analysis of structural rearrangements of the DNA substrates and uncleavable ligands during their interaction with Endo III using oligonucleotide duplexes containing 5,6-dihydrouracil, a natural abasic site, its tetrahydrofuran analogue, and undamaged duplexes carried fluorescent DNA base analogues 2-aminopurine and 1,3-diaza-2-oxophenoxazine as environment-sensitive reporter groups, pre-steady-state kinetic analysis, overview. Endo III induces several fast sequential conformational changes in DNA during binding, lesion recognition, and adjustment to a catalytically competent conformation. The glycosylase uses a multistep mechanism of damage recognition, which likely involves Gln41 and Leu81 as DNA lesion sensors
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.0000035 - 0.0014
5'-CTCTCCCTTC-5,6-dihydrouracil-CTCCTTTCCTCT-3'
0.00058 - 0.0013
5'-CTCTCCCTTC-8-oxo-7,8-dihydroguanine-CTCCTTTCCTCT-3'
0.0000023 - 0.00024
5'-GACAAGCGCAG-(5R,6S)-2'-deoxy-5,6-dihydroxyuridine-CAGCCGAACAC-3'
0.0000023 - 0.00021
5'-GACAAGCGCAG-(5S,6R)-2'-deoxy-5,6-dihydroxyuridine-CAGCCGAACAC-3'
0.000000083 - 0.000028
DNA containing apurinic/apyrimidinic sites
-
0.0000091 - 0.14
35 base pair oligonucleotide containing 5,6-dihydrouracil opposite A
-
0.0000036 - 0.000133
35 base pair oligonucleotide containing 5,6-dihydrouracil opposite G
-
0.0000097 - 0.004
35 base pair oligonucleotide containing 5,6-dihydroxy-5,6-dihydrothymine opposite A
-
0.0000059 - 0.00192
35 base pair oligonucleotide containing 5,6-dihydroxy-5,6-dihydrothymine opposite G
-
0.000088 - 0.011
AP-DNA
-
0.000038
DNA containing 5,6-dihydrothymidine/A
-
pH 7.5, 37°C
-
0.000316
DNA containing 5-hydroxy-2'-deoxyuridine/G
-
pH 7.5, 37°C
-
0.00000052
DNA containing apurinic sites
-
pH 7.5, 30°C
-
0.00000167
DNA containing O-benzylhydroxylamine
-
pH 7.5
-
0.00000284
DNA containing O-methylhydroxylamine
-
pH 7.5
-
0.0000013
DNA containing tetrahydrofuranyl/G
-
pH 7.5, 37°C
-
0.0000002 - 0.0000015
DNA containing thymine glycol
-
0.00000056
DNA containing urea
-
pH 7.5, 30°C
-
0.00025
DNA with 2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine/C
-
pH 7.5, 37°C
additional information
AP-DNA
-
0.0000035
5'-CTCTCCCTTC-5,6-dihydrouracil-CTCCTTTCCTCT-3'
wild-type, Nei placed opposite T
0.0000066
5'-CTCTCCCTTC-5,6-dihydrouracil-CTCCTTTCCTCT-3'
wild-type, Nei placed opposite C
0.0000085
5'-CTCTCCCTTC-5,6-dihydrouracil-CTCCTTTCCTCT-3'
wild-type, Nei placed opposite G
0.000038
5'-CTCTCCCTTC-5,6-dihydrouracil-CTCCTTTCCTCT-3'
mutant QLY/AAA, Nei placed opposite A
0.000042
5'-CTCTCCCTTC-5,6-dihydrouracil-CTCCTTTCCTCT-3'
mutant QLY/AAA, Nei placed opposite G
0.000054
5'-CTCTCCCTTC-5,6-dihydrouracil-CTCCTTTCCTCT-3'
mutant deltaQLY, Nei placed opposite G
0.000077
5'-CTCTCCCTTC-5,6-dihydrouracil-CTCCTTTCCTCT-3'
mutant Q261A, Nei placed opposite G
0.00014
5'-CTCTCCCTTC-5,6-dihydrouracil-CTCCTTTCCTCT-3'
mutant QLY/AAA, Nei placed opposite C
0.00016
5'-CTCTCCCTTC-5,6-dihydrouracil-CTCCTTTCCTCT-3'
mutant QLY/AAA, Nei placed opposite T
0.00016
5'-CTCTCCCTTC-5,6-dihydrouracil-CTCCTTTCCTCT-3'
wild-type, Nei placed opposite A
0.00026
5'-CTCTCCCTTC-5,6-dihydrouracil-CTCCTTTCCTCT-3'
mutant Q261A, Nei placed opposite A
0.00027
5'-CTCTCCCTTC-5,6-dihydrouracil-CTCCTTTCCTCT-3'
mutant Q261A, Nei placed opposite C
0.0014
5'-CTCTCCCTTC-5,6-dihydrouracil-CTCCTTTCCTCT-3'
mutant Q261A, Nei placed opposite T
0.00058
5'-CTCTCCCTTC-8-oxo-7,8-dihydroguanine-CTCCTTTCCTCT-3'
wild-type, Nei placed opposite G
0.00061
5'-CTCTCCCTTC-8-oxo-7,8-dihydroguanine-CTCCTTTCCTCT-3'
wild-type, Nei placed opposite A
0.00068
5'-CTCTCCCTTC-8-oxo-7,8-dihydroguanine-CTCCTTTCCTCT-3'
wild-type, Nei placed opposite C
0.0013
5'-CTCTCCCTTC-8-oxo-7,8-dihydroguanine-CTCCTTTCCTCT-3'
wild-type, Nei placed opposite T
0.0000023
5'-GACAAGCGCAG-(5R,6S)-2'-deoxy-5,6-dihydroxyuridine-CAGCCGAACAC-3'
wild-type, Nei placed opposite G
0.0000032
5'-GACAAGCGCAG-(5R,6S)-2'-deoxy-5,6-dihydroxyuridine-CAGCCGAACAC-3'
wild-type, Nei placed opposite A
0.0000044
5'-GACAAGCGCAG-(5R,6S)-2'-deoxy-5,6-dihydroxyuridine-CAGCCGAACAC-3'
wild-type, Nei placed opposite C
0.0000044
5'-GACAAGCGCAG-(5R,6S)-2'-deoxy-5,6-dihydroxyuridine-CAGCCGAACAC-3'
wild-type, Nei placed opposite T
0.000049
5'-GACAAGCGCAG-(5R,6S)-2'-deoxy-5,6-dihydroxyuridine-CAGCCGAACAC-3'
mutant QLY/AAA, Nei placed opposite G
0.000066
5'-GACAAGCGCAG-(5R,6S)-2'-deoxy-5,6-dihydroxyuridine-CAGCCGAACAC-3'
mutant deltaQLY, Nei placed opposite A
0.00019
5'-GACAAGCGCAG-(5R,6S)-2'-deoxy-5,6-dihydroxyuridine-CAGCCGAACAC-3'
mutant QLY/AAA, Nei placed opposite T
0.00024
5'-GACAAGCGCAG-(5R,6S)-2'-deoxy-5,6-dihydroxyuridine-CAGCCGAACAC-3'
mutant Q261A, Nei placed opposite G
0.0000023
5'-GACAAGCGCAG-(5S,6R)-2'-deoxy-5,6-dihydroxyuridine-CAGCCGAACAC-3'
wild-type, Nei placed opposite G
0.0000046
5'-GACAAGCGCAG-(5S,6R)-2'-deoxy-5,6-dihydroxyuridine-CAGCCGAACAC-3'
wild-type, Nei placed opposite T
0.0000047
5'-GACAAGCGCAG-(5S,6R)-2'-deoxy-5,6-dihydroxyuridine-CAGCCGAACAC-3'
wild-type, Nei placed opposite C
0.000016
5'-GACAAGCGCAG-(5S,6R)-2'-deoxy-5,6-dihydroxyuridine-CAGCCGAACAC-3'
wild-type, Nei placed opposite A
0.00021
5'-GACAAGCGCAG-(5S,6R)-2'-deoxy-5,6-dihydroxyuridine-CAGCCGAACAC-3'
mutant Q261A, Nei placed opposite G
0.000000083
DNA containing apurinic/apyrimidinic sites
wild-type, Nei placed opposite A
-
0.00000011
DNA containing apurinic/apyrimidinic sites
wild-type, Nei placed opposite G
-
0.000022
DNA containing apurinic/apyrimidinic sites
mutant QLY/AAA, Nei placed opposite G
-
0.000028
DNA containing apurinic/apyrimidinic sites
mutant QLY/AAA, Nei placed opposite A
-
0.0000091
35 base pair oligonucleotide containing 5,6-dihydrouracil opposite A
-
pH 7.6, 37°C, wild-type enzyme
-
0.14
35 base pair oligonucleotide containing 5,6-dihydrouracil opposite A
-
pH 7.6, 37°C, mutant enzyme R184A
-
0.0000036
35 base pair oligonucleotide containing 5,6-dihydrouracil opposite G
-
pH 7.6, 37°C, wild-type enzyme
-
0.000133
35 base pair oligonucleotide containing 5,6-dihydrouracil opposite G
-
pH 7.6, 37°C, mutant enzyme R184A
-
0.0000097
35 base pair oligonucleotide containing 5,6-dihydroxy-5,6-dihydrothymine opposite A
-
pH 7.6, 37°C, wild-type enzyme
-
0.004
35 base pair oligonucleotide containing 5,6-dihydroxy-5,6-dihydrothymine opposite A
-
pH 7.6, 37°C, mutant enzyme R184A
-
0.0000059
35 base pair oligonucleotide containing 5,6-dihydroxy-5,6-dihydrothymine opposite G
-
pH 7.6, 37°C, wild-type enzyme
-
0.00192
35 base pair oligonucleotide containing 5,6-dihydroxy-5,6-dihydrothymine opposite G
-
pH 7.6, 37°C, mutant enzyme R184A
-
0.000088
AP-DNA
-
wild-type in rAP-containing oligonucleotide cleavage assay
-
0.00012
AP-DNA
-
mutant Y72F in rAP-containing oligonucleotide cleavage assay
-
0.0075
AP-DNA
-
mutant Y72A in rAP-containing oligonucleotide cleavage assay
-
0.011
AP-DNA
-
mutant R37A in rAP-containing oligonucleotide cleavage assay
-
0.0000002
DNA containing thymine glycol
-
pH 7.5, 30°C
-
0.0000015
DNA containing thymine glycol
-
pH 7.5
-
additional information
AP-DNA
-
not detected in mutant E261Q in rAP-containing oligonucleotide cleavage assay
-
additional information
additional information
pre-steady-state kinetic analysis of structural rearrangements of the DNA substrates and uncleavable ligands during their interaction with Endo III
-
additional information
additional information
-
pre-steady-state kinetic analysis of structural rearrangements of the DNA substrates and uncleavable ligands during their interaction with Endo III
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.0000048 - 0.07167
5'-CTCTCCCTTC-5,6-dihydrouracil-CTCCTTTCCTCT-3'
0.00783 - 0.023
5'-CTCTCCCTTC-8-oxo-7,8-dihydroguanine-CTCCTTTCCTCT-3'
0.0025 - 0.0483
5'-GACAAGCGCAG-(5R,6S)-2'-deoxy-5,6-dihydroxyuridine-CAGCCGAACAC-3'
0.00567 - 0.0567
5'-GACAAGCGCAG-(5S,6R)-2'-deoxy-5,6-dihydroxyuridine-CAGCCGAACAC-3'
0.0015 - 0.01067
DNA containing apurinic/apyrimidinic sites
-
0.03 - 1.37
35 base pair oligonucleotide containing 5,6-dihydrouracil opposite A
-
0.043 - 0.17
35 base pair oligonucleotide containing 5,6-dihydrouracil opposite G
-
0.098
35 base pair oligonucleotide containing 5,6-dihydroxy-5,6-dihydrothymine opposite A
-
0.075 - 1.6
35 base pair oligonucleotide containing 5,6-dihydroxy-5,6-dihydrothymine opposite G
-
0.00417
4-hydroxy-5-N-methylformamidopyrimidine/C
-
pH 7.5, 37°C
-
0.0158
DNA containing 2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine/C
-
pH 7.5, 37°C
-
0.0267
DNA containing 5-hydroxy-2'-deoxyuridine/G
-
pH 7.5, 37°C
-
0.0283
DNA containing tetrahydrofuranyl/G
-
pH 7.5, 37°C
-
additional information
AP-DNA
-
not detected in mutant E261Q
-
0.0000048
5'-CTCTCCCTTC-5,6-dihydrouracil-CTCCTTTCCTCT-3'
mutant deltaQLY, Nei placed opposite G
0.000013
5'-CTCTCCCTTC-5,6-dihydrouracil-CTCCTTTCCTCT-3'
mutant Q261A, Nei placed opposite C
0.000015
5'-CTCTCCCTTC-5,6-dihydrouracil-CTCCTTTCCTCT-3'
mutant Q261A, Nei placed opposite G
0.000023
5'-CTCTCCCTTC-5,6-dihydrouracil-CTCCTTTCCTCT-3'
mutant Q261A, Nei placed opposite A
0.000065
5'-CTCTCCCTTC-5,6-dihydrouracil-CTCCTTTCCTCT-3'
mutant QLY/AAA, Nei placed opposite G
0.000067
5'-CTCTCCCTTC-5,6-dihydrouracil-CTCCTTTCCTCT-3'
mutant Q261A, Nei placed opposite T
0.00007167
5'-CTCTCCCTTC-5,6-dihydrouracil-CTCCTTTCCTCT-3'
mutant QLY/AAA, Nei placed opposite A
0.000583
5'-CTCTCCCTTC-5,6-dihydrouracil-CTCCTTTCCTCT-3'
wild-type, Nei placed opposite A
0.003167
5'-CTCTCCCTTC-5,6-dihydrouracil-CTCCTTTCCTCT-3'
wild-type, Nei placed opposite T
0.008167
5'-CTCTCCCTTC-5,6-dihydrouracil-CTCCTTTCCTCT-3'
wild-type, Nei placed opposite C
0.01167
5'-CTCTCCCTTC-5,6-dihydrouracil-CTCCTTTCCTCT-3'
mutant QLY/AAA, Nei placed opposite T
0.013
5'-CTCTCCCTTC-5,6-dihydrouracil-CTCCTTTCCTCT-3'
mutant QLY/AAA, Nei placed opposite C
0.07167
5'-CTCTCCCTTC-5,6-dihydrouracil-CTCCTTTCCTCT-3'
wild-type, Nei placed opposite G
0.00783
5'-CTCTCCCTTC-8-oxo-7,8-dihydroguanine-CTCCTTTCCTCT-3'
wild-type, Nei placed opposite A
0.01317
5'-CTCTCCCTTC-8-oxo-7,8-dihydroguanine-CTCCTTTCCTCT-3'
wild-type, Nei placed opposite G
0.01583
5'-CTCTCCCTTC-8-oxo-7,8-dihydroguanine-CTCCTTTCCTCT-3'
wild-type, Nei placed opposite C
0.023
5'-CTCTCCCTTC-8-oxo-7,8-dihydroguanine-CTCCTTTCCTCT-3'
wild-type, Nei placed opposite T
0.0025
5'-GACAAGCGCAG-(5R,6S)-2'-deoxy-5,6-dihydroxyuridine-CAGCCGAACAC-3'
mutant deltaQLY, Nei placed opposite A
0.00583
5'-GACAAGCGCAG-(5R,6S)-2'-deoxy-5,6-dihydroxyuridine-CAGCCGAACAC-3'
mutant Q261A, Nei placed opposite G
0.016
5'-GACAAGCGCAG-(5R,6S)-2'-deoxy-5,6-dihydroxyuridine-CAGCCGAACAC-3'
mutant QLY/AAA, Nei placed opposite G
0.02167
5'-GACAAGCGCAG-(5R,6S)-2'-deoxy-5,6-dihydroxyuridine-CAGCCGAACAC-3'
mutant QLY/AAA, Nei placed opposite T
0.03
5'-GACAAGCGCAG-(5R,6S)-2'-deoxy-5,6-dihydroxyuridine-CAGCCGAACAC-3'
wild-type, Nei placed opposite G
0.035
5'-GACAAGCGCAG-(5R,6S)-2'-deoxy-5,6-dihydroxyuridine-CAGCCGAACAC-3'
wild-type, Nei placed opposite A
0.04167
5'-GACAAGCGCAG-(5R,6S)-2'-deoxy-5,6-dihydroxyuridine-CAGCCGAACAC-3'
wild-type, Nei placed opposite C
0.0483
5'-GACAAGCGCAG-(5R,6S)-2'-deoxy-5,6-dihydroxyuridine-CAGCCGAACAC-3'
wild-type, Nei placed opposite T
0.00567
5'-GACAAGCGCAG-(5S,6R)-2'-deoxy-5,6-dihydroxyuridine-CAGCCGAACAC-3'
mutant Q261A, Nei placed opposite G
0.0112
5'-GACAAGCGCAG-(5S,6R)-2'-deoxy-5,6-dihydroxyuridine-CAGCCGAACAC-3'
wild-type, Nei placed opposite T
0.0115
5'-GACAAGCGCAG-(5S,6R)-2'-deoxy-5,6-dihydroxyuridine-CAGCCGAACAC-3'
wild-type, Nei placed opposite C
0.023
5'-GACAAGCGCAG-(5S,6R)-2'-deoxy-5,6-dihydroxyuridine-CAGCCGAACAC-3'
wild-type, Nei placed opposite A
0.0567
5'-GACAAGCGCAG-(5S,6R)-2'-deoxy-5,6-dihydroxyuridine-CAGCCGAACAC-3'
wild-type, Nei placed opposite G
0.0015
DNA containing apurinic/apyrimidinic sites
mutant QLY/AAA, Nei placed opposite A
-
0.00183
DNA containing apurinic/apyrimidinic sites
mutant QLY/AAA, Nei placed opposite G
-
0.00883
DNA containing apurinic/apyrimidinic sites
wild-type, Nei placed opposite G
-
0.01067
DNA containing apurinic/apyrimidinic sites
wild-type, Nei placed opposite A
-
0.03
35 base pair oligonucleotide containing 5,6-dihydrouracil opposite A
-
pH 7.6, 37°C, wild-type enzyme
-
1.37
35 base pair oligonucleotide containing 5,6-dihydrouracil opposite A
-
pH 7.6, 37°C, mutant enzyme R184A
-
0.043
35 base pair oligonucleotide containing 5,6-dihydrouracil opposite G
-
pH 7.6, 37°C, wild-type enzyme
-
0.17
35 base pair oligonucleotide containing 5,6-dihydrouracil opposite G
-
pH 7.6, 37°C, mutant enzyme R184A
-
0.098
35 base pair oligonucleotide containing 5,6-dihydroxy-5,6-dihydrothymine opposite A
-
pH 7.6, 37°C, wild-type enzyme
-
0.098
35 base pair oligonucleotide containing 5,6-dihydroxy-5,6-dihydrothymine opposite A
-
pH 7.6, 37°C, mutant enzyme R184A
-
0.075
35 base pair oligonucleotide containing 5,6-dihydroxy-5,6-dihydrothymine opposite G
-
pH 7.6, 37°C, wild-type enzyme
-
1.6
35 base pair oligonucleotide containing 5,6-dihydroxy-5,6-dihydrothymine opposite G
-
pH 7.6, 37°C, mutant enzyme R184A
-
72
AP-DNA
-
mutant Y72A
-
354
AP-DNA
-
mutant Y72F
-
7680
AP-DNA
-
mutant R37A
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Nakabeppu, Y.; Sekiguchi, M.
Physical association of pyrimidine dimer DNA glycosylase and apurinic/apyrimidinic DNA endonuclease essential for repair of ultraviolet-damaged DNA
Proc. Natl. Acad. Sci. USA
78
2742-2746
1981
Tequatrovirus T4, Escherichia coli
brenda
Kim, J.; Linn, S.
The mechanism of action of E. coli endonuclease III and T4 UV endonuclease (endonuclease V) at AP sites
Nucleic Acids Res.
16
1135-1141
1988
Tequatrovirus T4, Escherichia coli
brenda
Hatahet, Z.; Kow, Y.W.; Purmal, A.A.; Cunningham, R.P.; Wallace, S.S.
New substrates for old enzymes. 5-Hydroxy-2'-deoxycytidine and 5-hydroxy-2'-deoxyuridine are substrates for Escherichia coli endonuclease III and formamidopyrimidine DNA N-glycosylase, while 5-hydroxy-2'-deoxyuridine is a substrate for uracil DNA N-glycosylase
J. Biol. Chem.
269
18814-18820
1994
Escherichia coli
brenda
Takeshita, M.; Chang, C.N.; Johnson, F.; Will, S.; Grollman, A.P.
Oligodeoxynucleotides containing synthetic abasic sites. Model substrates for DNA polymerases and apurinic/apyrimidinic endonucleases
J. Biol. Chem.
262
10171-10179
1982
Escherichia coli
brenda
Bailly, V.; Verly, W.G.
Escherichia coli endonuclease III is not an endonuclease but a beta-elimination catalyst
Biochem. J.
242
565-572
1987
Escherichia coli
brenda
Manoharan, M.; Mazumder, A.; Ranson, S.C.; Gerlt, J.A.; Bolton, P.H.
Mechanism of UV endonuclease V cleavage of abasic sites in DNA determined by 13C labeling
J. Am. Chem. Soc.
110
2690-2691
1988
Tequatrovirus T4, Escherichia coli
-
brenda
Bailly, V.; Verly, W.G.
AP endonuleases an AP lyases
Nucleic Acids Res.
17
3617-3618
1989
Escherichia coli
brenda
Gossard, F.; Verly, W.G.
Properties of the main endonucleases specific for apurinic sites of Escherichia coli (endonuclease VI)
Eur. J. Biochem.
82
321-332
1978
Escherichia coli
brenda
Katcher, H.L.; Wallace, S.S.
Characterization of Escherichia coli x-ray endonuclease, endonulease II
Biochemistry
22
4071-4081
1983
Escherichia coli
brenda
Kow, Y.W.; Wallace, S.S.
Mechanismm of action of Escherichia coli endonuclease III
Biochemistry
26
8200-8206
1987
Escherichia coli
brenda
Asahara, H.; Wistort, P.M.; Bank, J.F.; Bakerian, R.H.; Cunningham, R.P.
Purification and characterization of Escherichia coli endonuclease III from the cloned m gene
Biochemistry
28
4444-4449
1989
Escherichia coli
brenda
Cunningham, R.P.; Asahara, H.; Bank, J.F.; Scholes, C.P.; Salerno, J.C.; Surerus, K.; Mnck, E.; McCracken, J.; Peisach, J.; Emptage, M.H.
Endonuclease is an iron-sulfur protein
Biochemistry
28
4450-4455
1989
Escherichia coli
brenda
Dizdaroglu, M.; Laval, J.; Boiteux, S.
Substrate specificity of the Escherichia coli endonuclease III: Excision of thymine- and cytosine-derived lesions in DNA produced by radiation-generated free radicals
Biochemistry
32
12105-12111
1993
Escherichia coli
brenda
Fu, W.; O'Handley, S.; Cunningham, R.P.; Johnson, M.K.
The role of the iron-sulfur cluster in Escherichia cloli endonuclease III. A resonance Raman strudy
J. Biol. Chem.
267
16135-16137
1992
Escherichia coli
brenda
Kuo, C.F.; McRee, D.E.; Cunningham, R.P.; Tainer, J.A.
Crystallization and crystallographic characterization of the iron-sulfur-containing DNA-repair enzyme endonuclease III form Escherichia coli
J. Mol. Biol.
227
347-351
1992
Escherichia coli
brenda
Warner, H.R.; Persson, M.L.; Bensen, R.J.; Mosbaugh, D.W.; Linn, S.
Selective inhibition by harmane of apurinic/apyrimidinic endonuclease activity of phage T4-induced UV endonuclease
Nucleic Acids Res.
9
6083-6092
1981
Tequatrovirus T4, Escherichia coli, Homo sapiens
brenda
Chaudhry, M.A.; Weinfeld, M.
Reactivity of human apurinic/apyrimidinic endonuclease and Escherichia coli exonuclease III with bistranded abasic sites in DNA
J. Biol. Chem.
272
15650-15655
1997
Escherichia coli, Homo sapiens
brenda
Chaudhry, M.A.; Weinfeld, M.
The action of Escherichia coli endonuclease III on multiply damaged sites in DNA
J. Mol. Biol.
249
914-922
1995
Escherichia coli
brenda
Ishchenko, A.A.; Sanz, G.; Privezentzev, C.V.; Maksimenko, A.V.; Saparbaev, M.
Characterisation of new substrate specificities of Escherichia coli and Saccharomyces cerevisiae AP endonucleases
Nucleic Acids Res.
31
6344-6353
2003
Saccharomyces cerevisiae, Escherichia coli
brenda
Pope, M.A.; Porello, S.L.; David, S.S.
Escherichia coli apurinic-apyrimidinic endonucleases enhance the turnover of the adenine glycosylase MutY with G:A substrates
J. Biol. Chem.
277
22605-22615
2002
Escherichia coli, Homo sapiens
brenda
Purmal, A.A.; Rabow, L.E.; Lampman, G.W.; Cunningham, R.P.; Kow, Y.W.
A common mechanism of action for the N-glycosylase activity of DNA N-glycosylase/AP lyases from E. coli and T4
Mutat. Res.
364
193-207
1996
Tequatrovirus T4, Escherichia coli
brenda
Greenberg, M.M.; Weledji, Y.N.; Kim, J.; Bales, B.C.
Repair of oxidized abasic sites by exonuclease III, endonuclease IV, and endonuclease III
Biochemistry
43
8178-8183
2004
Escherichia coli
brenda
Katafuchi, A.; Nakano, T.; Masaoka, A.; Terato, H.; Iwai, S.; Hanaoka, F.; Ide, H.
Differential specificity of human and Escherichia coli endonuclease III and VIII homologues for oxidative base lesions
J. Biol. Chem.
279
14464-14471
2004
Escherichia coli
brenda
Watanabe, T.; Blaisdell, J.O.; Wallace, S.S.; Bond, J.P.
Engineering functional changes in Escherichia coli endonuclease III based on phylogenetic and structural analyses
J. Biol. Chem.
280
34378-34384
2005
Escherichia coli
brenda
Doi, Y.; Katafuchi, A.; Fujiwara, Y.; Hitomi, K.; Tainer, J.A.; Ide, H.; Iwai, S.
Synthesis and characterization of oligonucleotides containing 2'-fluorinated thymidine glycol as inhibitors of the endonuclease III reaction
Nucleic Acids Res.
34
1540-1551
2006
Escherichia coli
brenda
Kropachev, K.Y.; Zharkov, D.O.; Grollman, A.P.
Catalytic mechanism of Escherichia coli endonuclease VIII: roles of the intercalation loop and the zinc finger
Biochemistry
45
12039-12049
2006
Escherichia coli (P50465), Escherichia coli
brenda
Kaneda, K.; Ohishi, K.; Sekiguchi, J.; Shida, T.
Characterization of the AP endonucleases from Thermoplasma volcanium and Lactobacillus plantarum: Contributions of two important tryptophan residues to AP site recognition
Biosci. Biotechnol. Biochem.
70
2213-2221
2006
Escherichia coli, Lactiplantibacillus plantarum, Thermoplasma volcanium
brenda
Garcin, E.D.; Hosfield, D.J.; Desai, S.A.; Haas, B.J.; Bjoeras, M.; Cunningham, R.P.; Tainer, J.A.
DNA apurinic-apyrimidinic site binding and excision by endonuclease IV
Nat. Struct. Mol. Biol.
15
515-522
2008
Escherichia coli, Escherichia coli BW565DE3
brenda
Zhang-Akiyama, Q.M.; Morinaga, H.; Kikuchi, M.; Yonekura, S.; Sugiyama, H.; Yamamoto, K.; Yonei, S.
KsgA, a 16S rRNA adenine methyltransferase, has a novel DNA glycosylase/AP lyase activity to prevent mutations in Escherichia coli
Nucleic Acids Res.
37
2116-2125
2009
Escherichia coli, Escherichia coli KSR7
brenda
Kuznetsov, N.A.; Kladova, O.A.; Kuznetsova, A.A.; Ishchenko, A.A.; Saparbaev, M.K.; Zharkov, D.O.; Fedorova, O.S.
Conformational dynamics of DNA repair by Escherichia coli endonuclease III
J. Biol. Chem.
290
14338-14349
2015
Escherichia coli (P0AB83), Escherichia coli
brenda