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EC Tree
IUBMB Comments The enzyme from rice (Oryza sativa), expressed in Escherichia coli, also produces ent-sandaracopimara-8(14),15-diene from ent-copalyl diphosphate, another naturally occuring copalyl isomer in rice (cf. ent-sandaracopimaradiene synthase, EC 4.2.3.29).
The expected taxonomic range for this enzyme is: Oryza sativa
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9alpha-copalyl diphosphate = (12E)-9alpha-labda-8(17),12,14-triene + diphosphate
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9alpha-copalyl-diphosphate diphosphate-lyase [(12E)-9alpha-labda-8(17),12,14-triene-forming]
The enzyme from rice (Oryza sativa), expressed in Escherichia coli, also produces ent-sandaracopimara-8(14),15-diene from ent-copalyl diphosphate, another naturally occuring copalyl isomer in rice (cf. ent-sandaracopimaradiene synthase, EC 4.2.3.29).
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syn-copalyl diphosphate
syn-labda-8(17),12E,14-triene + diphosphate
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i.e. (4aS,5R,8aS)-1,1,4a-trimethyl-6-methylidene-5-[(2E)-3-methylpenta-2,4-dien-1-yl]decahydronaphthalene
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additional information
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additionally converts ent-copalyl diphosphate to ent-pimara-8(14),15-diene, reaction of EC 4.2.3.29
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0.005
syn-copalyl diphosphate
22°C, pH not specified in the publication
additional information
additional information
KM value for substrate ent-copalyl diphosphate is 0.0006 mM
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0.00083
syn-copalyl diphosphate
22°C, pH not specified in the publication
additional information
additional information
Kcat value for substrate ent-copalyl diphosphate is 0.00033 per s
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0.17
syn-copalyl diphosphate
22°C, pH not specified in the publication
additional information
additional information
Kcat/KM value for substrate ent-copalyl diphosphate is 0.55 per s and mM
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SwissProt
brenda
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KSL10_ORYSJ
815
0
91930
Swiss-Prot
other Location (Reliability: 4 )
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expression as glutathione-S-transferase fusion protein
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Morrone, D.; Hillwig, M.L.; Mead, M.E.; Lowry, L.; Fulton, D.B.; Peters, R.J.
Evident and latent plasticity across the rice diterpene synthase family with potential implications for the evolution of diterpenoid metabolism in the cereals
Biochem. J.
435
589-595
2011
Oryza sativa (Q2QQJ5)
brenda
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Enzyme Nomenclature
4.2.3.99 labdatriene synthase
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