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IUBMB CommentsRequires Mg2+. The enzyme forms 5-epi-alpha-selinene possibly via germecrene A or a 1,6-hydride shift mechanism.
The enzyme appears in viruses and cellular organisms
Synonyms
NP1, NS1, terpene synthase,
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NS1
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terpene synthase
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(2E,6E)-farnesyl diphosphate = 5-epi-alpha-selinene + diphosphate
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(2Z,6E)-farnesyl-diphosphate diphosphate-lyase (cyclizing, 5-epi-alpha-selinene-forming)
Requires Mg2+. The enzyme forms 5-epi-alpha-selinene possibly via germecrene A or a 1,6-hydride shift mechanism.
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(2E,6E)-farnesyl diphosphate
8a-epi-alpha-selinene + diphosphate
(E,E)-farnesyl diphosphate
8a-epi-alpha-selinene + diphosphate
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?
(2E,6E)-farnesyl diphosphate
8a-epi-alpha-selinene + diphosphate
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(2E,6E)-farnesyl diphosphate
8a-epi-alpha-selinene + diphosphate
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0.0034
(2E,6E)-farnesyl diphosphate
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in 10 mM Tris-HCl, 10 mM MgCl2, 1 mM beta-mercaptoethanol, pH 8.0, at 30°C
0.0025
(E,E)-farnesyl diphosphate
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in 10 mM Tris-HCl, 10 mM MgCl2, 1 mM beta-mercaptoethanol, pH 8.0, at 30°C
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0.044
(2E,6E)-farnesyl diphosphate
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in 10 mM Tris-HCl, 10 mM MgCl2, 1 mM beta-mercaptoethanol, pH 8.0, at 30°C
0.063
(E,E)-farnesyl diphosphate
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in 10 mM Tris-HCl, 10 mM MgCl2, 1 mM beta-mercaptoethanol, pH 8.0, at 30°C
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13000
(2E,6E)-farnesyl diphosphate
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in 10 mM Tris-HCl, 10 mM MgCl2, 1 mM beta-mercaptoethanol, pH 8.0, at 30°C
24000
(E,E)-farnesyl diphosphate
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in 10 mM Tris-HCl, 10 mM MgCl2, 1 mM beta-mercaptoethanol, pH 8.0, at 30°C
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brenda
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GERAS_NOSP7
Nostoc punctiforme (strain ATCC 29133 / PCC 73102)
323
0
38123
Swiss-Prot
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A0A5B7VA49_9ACTN
203
0
23488
TrEMBL
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A0A0W0V2J2_9GAMM
420
0
48303
TrEMBL
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A0A3G4VLN3_9ACTN
866
0
92426
TrEMBL
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A0A2S9YUV6_9DELT
326
0
36546
TrEMBL
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A0A445NHX1_STRNE
349
0
38744
TrEMBL
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A0A1S9D116_9GAMM
309
0
35335
TrEMBL
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A0A3N6EVJ5_9ACTN
323
0
36195
TrEMBL
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Talon metal affinity column chromatography
Talon metal affinity column chromatography
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Talon metal affinity column chromatography
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expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli BL21(DE3) cells
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expressed in Escherichia coli BL21(DE3) cells
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Agger, S.A.; Lopez-Gallego, F.; Hoye, T.R.; Schmidt-Dannert, C.
Identification of sesquiterpene synthases from Nostoc punctiforme PCC 73102 and Nostoc sp. strain PCC 7120
J. Bacteriol.
190
6084-6096
2008
Nostoc punctiforme, Nostoc sp., Nostoc sp. PCC 712
brenda
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4.2.3.90 5-epi-alpha-selinene synthase
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