Information on EC 4.2.3.90 - 5-epi-alpha-selinene synthase
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The expected taxonomic range for this enzyme is: Nostoc
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5-epi-alpha-selinene synthase
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(2E,6E)-farnesyl diphosphate = 5-epi-alpha-selinene + diphosphate
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Sesquiterpenoid and triterpenoid biosynthesis
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(2Z,6E)-farnesyl-diphosphate diphosphate-lyase (cyclizing, 5-epi-alpha-selinene-forming)
Requires Mg2+. The enzyme forms 5-epi-alpha-selinene possibly via germecrene A or a 1,6-hydride shift mechanism.
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NS1

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terpene synthase

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(2E,6E)-farnesyl diphosphate
8a-epi-alpha-selinene + diphosphate
(E,E)-farnesyl diphosphate
8a-epi-alpha-selinene + diphosphate
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(2E,6E)-farnesyl diphosphate

8a-epi-alpha-selinene + diphosphate
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(2E,6E)-farnesyl diphosphate
8a-epi-alpha-selinene + diphosphate
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0.0034
(2E,6E)-farnesyl diphosphate
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in 10 mM Tris-HCl, 10 mM MgCl2, 1 mM beta-mercaptoethanol, pH 8.0, at 30°C
0.0025
(E,E)-farnesyl diphosphate
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in 10 mM Tris-HCl, 10 mM MgCl2, 1 mM beta-mercaptoethanol, pH 8.0, at 30°C
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0.044
(2E,6E)-farnesyl diphosphate
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in 10 mM Tris-HCl, 10 mM MgCl2, 1 mM beta-mercaptoethanol, pH 8.0, at 30°C
0.063
(E,E)-farnesyl diphosphate
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in 10 mM Tris-HCl, 10 mM MgCl2, 1 mM beta-mercaptoethanol, pH 8.0, at 30°C
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13000
(2E,6E)-farnesyl diphosphate
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in 10 mM Tris-HCl, 10 mM MgCl2, 1 mM beta-mercaptoethanol, pH 8.0, at 30°C
24000
(E,E)-farnesyl diphosphate
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in 10 mM Tris-HCl, 10 mM MgCl2, 1 mM beta-mercaptoethanol, pH 8.0, at 30°C
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Talon metal affinity column chromatography
Talon metal affinity column chromatography

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Talon metal affinity column chromatography
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expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli BL21(DE3) cells

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expressed in Escherichia coli BL21(DE3) cells
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GERAS_NOSP7
Nostoc punctiforme (strain ATCC 29133 / PCC 73102)
323
38123
Swiss-Prot
A0A1S9D116_9GAMM
309
35335
TrEMBL
A0A2S9Y7M9_9DELT
354
39881
TrEMBL
A0A2P8ASJ2_9ACTN
319
34994
TrEMBL
V5BIL8_9GAMM
331
37742
TrEMBL
A0A1B9EMW7_9ACTN
358
40298
TrEMBL
A0A1Y2N8F8_STRPT
337
38048
TrEMBL
A0A1K2G147_9ACTN
334
37418
TrEMBL
A0A2S9YUV6_9DELT
326
36546
TrEMBL
A0A2S9XR46_9DELT
355
39778
TrEMBL
A0A2P8AWU2_9ACTN
316
33925
TrEMBL
A0A1I9Z994_9NOCA
342
39232
TrEMBL
A0A1V2MHX8_9ACTN
356
40170
TrEMBL
A0A0W0V2J2_9GAMM
420
48303
TrEMBL
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Agger, S.A.; Lopez-Gallego, F.; Hoye, T.R.; Schmidt-Dannert, C.
Identification of sesquiterpene synthases from Nostoc punctiforme PCC 73102 and Nostoc sp. strain PCC 7120
J. Bacteriol.
190
6084-6096
2008
Nostoc punctiforme, Nostoc sp., Nostoc sp. PCC 712
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