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Information on EC 4.2.3.89 - (+)-beta-caryophyllene synthase for references in articles please use BRENDA:EC4.2.3.89Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
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The expected taxonomic range for this enzyme is: Streptomyces griseus
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(+)-beta-caryophyllene synthase
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(2E,6E)-farnesyl diphosphate = (+)-beta-caryophyllene + diphosphate
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Sesquiterpenoid and triterpenoid biosynthesis
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(2Z,6E)-farnesyl-diphosphate diphosphate-lyase [cyclizing, (+)-beta-caryophyllene-forming]
A multifunctional enzyme which also converts the (+)-beta-caryophyllene to (+)-caryolan-1-ol (see EC 4.2.1.138, (+)-caryolan-1-ol synthase). cf. EC 4.2.3.57 (-)-beta-caryophyllene synthase.
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GcoA
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brenda
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(2E,6E)-farnesyl diphosphate + H2O
(+)-beta-caryophyllene + diphosphate
(2E,6E)-farnesyl diphosphate + H2O
(+)-beta-caryophyllene + diphosphate
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(2E,6E)-farnesyl diphosphate + H2O
(+)-beta-caryophyllene + diphosphate
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Mg2+
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more than 5 mM MgCl2 is required for maximum activity. The enzyme reaction is unable to proceed without MgCl2
Mn2+
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38% activity (relative to the activity in the presence of Mg2+) is retained in the presence of 5 mM Mn2+
additional information
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no activity is observed for Fe2+, Co2+, Zn2+, Ni2+, or Cu2+
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0.0000956
(2E,6E)-farnesyl diphosphate
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at pH 7.5 and 30°C in the presence of 5 mM MgCl2
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0.025
(2E,6E)-farnesyl diphosphate
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at pH 7.5 and 30°C in the presence of 5 mM MgCl2
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brenda
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41000
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x * 41000, SDS-PAGE
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x * 41000, SDS-PAGE
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x * 41000, SDS-PAGE
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Ni-NTA column chromatography
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expressed in Streptomyces lividans strain TK21 and as N-terminally histidine-tagged enzyme in Escherichia coli BL21(DE3) cells
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GCOA_STRGG
Streptomyces griseus subsp. griseus (strain JCM 4626 / NBRC 13350)
335
37906
Swiss-Prot
A0A0B8NFL0_9NOCA
326
36677
TrEMBL
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Nakano, C.; Horinouchi, S.; Ohnishi, Y.
Characterization of a novel sesquiterpene cyclase involved in (+)-caryolan-1-ol biosynthesis in Streptomyces griseus
J. Biol. Chem.
286
27980-27987
2011
Streptomyces griseus, Streptomyces griseus IFO 13350
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