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Information on EC 4.2.3.86 - 7-epi-alpha-selinene synthase and Organism(s) Vitis vinifera and UniProt Accession Q6Q3H2

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EC Tree
     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.3 Acting on phosphates
                4.2.3.86 7-epi-alpha-selinene synthase
IUBMB Comments
The recombinant enzyme from Vitis vinifera forms 49.5% (+)-valencene (cf. EC 4.2.3.73, valencene synthase) and 35.5% (-)-7-epi-alpha-selinene. Initial cyclization gives (+)-germacrene A in an enzyme bound form which is not released to the medium.
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This record set is specific for:
Vitis vinifera
UNIPROT: Q6Q3H2
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The taxonomic range for the selected organisms is: Vitis vinifera
The expected taxonomic range for this enzyme is: Vitis vinifera
Synonyms
vvvalcs, vvpnseint, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
selinene synthase
AM459143
-
VvPNSeInt
AM459143
-
PATHWAY SOURCE
PATHWAYS
-
-
SYSTEMATIC NAME
IUBMB Comments
(2E,6E)-farnesyl-diphosphate diphosphate-lyase (7-epi-alpha-selinene-forming)
The recombinant enzyme from Vitis vinifera forms 49.5% (+)-valencene (cf. EC 4.2.3.73, valencene synthase) and 35.5% (-)-7-epi-alpha-selinene. Initial cyclization gives (+)-germacrene A in an enzyme bound form which is not released to the medium.
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(2E,6E)-farnesyl diphosphate
7-epi-alpha-selinene + diphosphate
show the reaction diagram
(2E,6E)-farnesyl diphosphate
alpha-guaiene + diphosphate
show the reaction diagram
AM459143
-
-
-
?
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
(2E,6E)-farnesyl diphosphate
7-epi-alpha-selinene + diphosphate
show the reaction diagram
the recombinant enzyme from Vitis vinifera forms 49.5% (+)-valencene (cf EC 4.2.3.73, valencene synthase) and 35.5% (-)-7-epi-alpha-selinene
-
-
?
(2E,6E)-farnesyl diphosphate
alpha-guaiene + diphosphate
show the reaction diagram
AM459143
-
-
-
?
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.8
calculated
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
transcripts become detectable in September, almost two months after flowering, then increase and reach a maximum at the final sample date in early October when the fruit is at peak maturity
Manually annotated by BRENDA team
pollen grain, during bloom
Manually annotated by BRENDA team
AM459143
-
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
additional information
the enzyme localizes close to lipid bodies within the maturing microspore
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
AM459143
the enzyme produces alpha-guaiene, a precursor of rotundone
additional information
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
TPSVS_VITVI
556
0
64432
Swiss-Prot
other Location (Reliability: 5)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
64400
x * 64400, calculated from sequence
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 64400, calculated from sequence
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
His-tagged VvValCS is expressed in Escherichia coli
construction of the synthetic terpene synthase construct from the sequence of alpga-guaiene synthase (EC 4.2.3.87), sequence comparisons and phylogenetic analysis, the recombinant enzyme is transiently expressed in Nicotiana benthamiana leaves and produces mainly alpha-guaiene and alpha-bulnesene, and also lower amounts of alpha-copaene, alpha-gurjunene, gamm-gurlunene, epiglobulol, epi-?alpha-selinene, and pogostol. Selina-4(14),11-diene, alpha-selinene, delta-cadinene, and intermedeol are not found
AM459143
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
transcript levels are low in open pre-anthesis flowers, flowers after anthesis, or at early onset of fruit development
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Luecker, J.; Bowen, P.; Bohlmann, J.
Vitis vinifera terpenoid cyclases: functional identification of two sesquiterpene synthase cDNAs encoding (+)-valencene synthase and (-)-germacrene D synthase and expression of mono- and sesquiterpene synthases in grapevine flowers and berries
Phytochemistry
65
2649-2659
2004
Vitis vinifera (Q6Q3H2)
Manually annotated by BRENDA team
Martin, D.M.; Toub, O.; Chiang, A.; Lo, B.C.; Ohse, S.; Lund, S.T.; Bohlmann, J.
The bouquet of grapevine (Vitis vinifera L. cv. Cabernet Sauvignon) flowers arises from the biosynthesis of sesquiterpene volatiles in pollen grains
Proc. Natl. Acad. Sci. USA
106
7245-7250
2009
Vitis vinifera (Q6Q3H2), Vitis vinifera L. cv. Cabernet Sauvignon (Q6Q3H2)
Manually annotated by BRENDA team
Drew, D.P.; Andersen, T.B.; Sweetman, C.; M?ller, B.L.; Ford, C.; Simonsen, H.T.
Two key polymorphisms in a newly discovered allele of the Vitis vinifera TPS24 gene are responsible for the production of the rotundone precursor ?-guaiene
J. Exp. Bot.
67
799-808
2016
Vitis vinifera (AM459143), Vitis vinifera
Manually annotated by BRENDA team