The recombinant enzyme from Vitis vinifera forms 49.5% (+)-valencene (cf. EC 4.2.3.73, valencene synthase) and 35.5% (-)-7-epi-alpha-selinene. Initial cyclization gives (+)-germacrene A in an enzyme bound form which is not released to the medium.
Specify your search results
The expected taxonomic range for this enzyme is: Vitis vinifera
The recombinant enzyme from Vitis vinifera forms 49.5% (+)-valencene (cf. EC 4.2.3.73, valencene synthase) and 35.5% (-)-7-epi-alpha-selinene. Initial cyclization gives (+)-germacrene A in an enzyme bound form which is not released to the medium.
male flower parts of grapevines are responsible for sesquiterpenoid floral scent formation. Sesquiterpene volatiles, which are emitted with a light-dependent diurnal pattern early in the morning at prebloom and bloom, are localized to anthers and, more specifically, within the developing pollen grains
transcripts become detectable in September, almost two months after flowering, then increase and reach a maximum at the final sample date in early October when the fruit is at peak maturity
molecular modelling of the two enzymes, selinene synthase (EC 4.2.3.86) and alpha-guayene synthase (EC 4.2.3.87) based on the TEAS template structure reveals that two of the varying amino acid positions are directly located in the active site, proximal to the location of FPP binding and subsequent catalysis, while the other four amino acid differences are located more peripherally, structure-function analysis. The S414 and M530 residues both contribute to the internal binding site of farnesyl diphosphate and are located on separate alpha-helices that contribute to the formation of the internal cavity comprising the FPP substrate-binding site
molecular modelling of the two enzymes, selinene synthase (EC 4.2.3.86) and alpha-guayene synthase (EC 4.2.3.87) based on the TEAS template structure reveals that two of the varying amino acid positions are directly located in the active site, proximal to the location of FPP binding and subsequent catalysis, while the other four amino acid differences are located more peripherally, structure-function analysis. The S414 and M530 residues both contribute to the internal binding site of farnesyl diphosphate and are located on separate alpha-helices that contribute to the formation of the internal cavity comprising the FPP substrate-binding site
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
construction of the synthetic terpene synthase construct from the sequence of alpga-guaiene synthase (EC 4.2.3.87), sequence comparisons and phylogenetic analysis, the recombinant enzyme is transiently expressed in Nicotiana benthamiana leaves and produces mainly alpha-guaiene and alpha-bulnesene, and also lower amounts of alpha-copaene, alpha-gurjunene, gamm-gurlunene, epiglobulol, epi-?alpha-selinene, and pogostol. Selina-4(14),11-diene, alpha-selinene, delta-cadinene, and intermedeol are not found
Vitis vinifera terpenoid cyclases: functional identification of two sesquiterpene synthase cDNAs encoding (+)-valencene synthase and (-)-germacrene D synthase and expression of mono- and sesquiterpene synthases in grapevine flowers and berries
Two key polymorphisms in a newly discovered allele of the Vitis vinifera TPS24 gene are responsible for the production of the rotundone precursor ?-guaiene
BRENDA home
History
4.2.3.86 7-epi-alpha-selinene synthase
more
top
