Any feedback?
Information on EC 4.2.3.82 - alpha-santalene synthase and Organism(s) Santalum album and UniProt Accession E3W202
for references in articles please use BRENDA:EC4.2.3.82Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
4.2.3.82 alpha-santalene synthaseIUBMB Comments
The enzyme synthesizes a mixture of sesquiterpenoids from (2E,6E)-farnesyl diphosphate. As well as (+)-alpha-santalene, (-)-beta-santalene and (-)-exo-alpha-bergamotene are formed with traces of (+)-epi-beta-santalene. See EC 4.2.3.83 [(-)-beta-santalene synthase], and EC 4.2.3.81 [(-)-exo-alpha-bergamotene synthase]. cf. EC 4.2.3.50 alpha-santalene synthase [(2Z,6Z)-farnesyl diphosphate cyclizing]
Specify your search results
Word Map
- 4.2.3.82
-
terpene
- sandalwood
-
sesquiterpene
-
santalols
- synthases
- santalum
- album
- mevalonate
- clausena
- lansium
- pyrophosphate
-
fed-batch
-
cyclization
- sesquiterpenoids
-
monoterpene
-
heartwood
-
unigenes
- sassy
-
bergamotene
- synthesis
The taxonomic range for the selected organisms is: Santalum album
The enzyme appears in selected viruses and cellular organisms
The enzyme appears in selected viruses and cellular organisms
Synonyms
santalene synthase, sansyn, 
more
topSYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
PATHWAY SOURCE
PATHWAYS
-
-
SYSTEMATIC NAME
IUBMB Comments
(2E,6E)-farnesyl diphosphate lyase (cyclizing, (+)-alpha-santalene-forming)
The enzyme synthesizes a mixture of sesquiterpenoids from (2E,6E)-farnesyl diphosphate. As well as (+)-alpha-santalene, (-)-beta-santalene and (-)-exo-alpha-bergamotene are formed with traces of (+)-epi-beta-santalene. See EC 4.2.3.83 [(-)-beta-santalene synthase], and EC 4.2.3.81 [(-)-exo-alpha-bergamotene synthase]. cf. EC 4.2.3.50 alpha-santalene synthase [(2Z,6Z)-farnesyl diphosphate cyclizing]
SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
(2E,6E)-farnesyl diphosphate
alpha-santalene + diphosphate
-
products are alpha-santalene, beta-, and epi-beta-santalene, exo-alpha-bergamotene, as well as traces of alpha- and beta-farnesene
-
?
(2E,6E)-farnesyl diphosphate
alpha-santalene + diphosphate
-
synthesis of a mixture of sesquiterpenes namely alpha-santalene, beta-santalene, epi-beta-santalene and alpha-bergamotene
-
?
(2E,6E)-farnesyl diphosphate
(+)-alpha-santalene + diphosphate
-
-
-
?
(2E,6E)-farnesyl diphosphate
(+)-alpha-santalene + diphosphate
when incubated in the presence of 10 mM Mg2+ the enzyme converts (2E,6E)-farnesyl diphosphate into alpha-santalene, beta-santalene and epi-beta-santalene,alpha-exo-bergamotene, as well as traces of alpha-farnesene and beta-farnesene. Incubations with Mn2+ yields mainly alpha-exo-bergamotene
-
-
?
(2E,6E)-farnesyl diphosphate
(+)-alpha-santalene + diphosphate
when incubated in the presence of 10 mM Mg2+ the enzyme converts (2Z,6Z)-farnesyl diphosphate into alpha-endo-bergamotene, alpha-santalene, (Z)-beta-farnesene, epi-beta-santalene and beta-santalene
-
-
?
additional information
?
-
Santalum album Ssy also accepts (2Z,6Z)-farnesyl diphosphate as substrate, producing a mixture of alpha-endo-bergamotene, alpha-santalene, (Z)-beta-farnesene, epi-beta-santalene, and beta-santalene. With substrate geranyl diphosphate, products are linalool, geraniol, and terpineol along with traces of alpha-pinene and camphene. The product profile resulting from incubations of SaSSy with (E,E)-FPP resembles the authentic essential oil of Santalum album more closely than that of the (Z,Z)-FPP incubations
-
-
?
additional information
?
-
Santalum album Ssy also accepts (2Z,6Z)-farnesyl diphosphate as substrate, producing a mixture of alpha-endo-bergamotene, alpha-santalene, (Z)-beta-farnesene, epi-beta-santalene, and beta-santalene. With substrate geranyl diphosphate, products are linalool, geraniol, and terpineol along with traces of alpha-pinene and camphene
-
-
?
additional information
?
-
the enzyme produces high amounts of (+)-alpha-santalene and (-)-beta-santalene, also (-)-exo-alpha-bergamotene, and low amounts of (-)-epi-beta-santalene from (2E,6E)-farnesyl diphosphate
-
-
?
additional information
?
-
the enzyme produces high amounts of (+)-alpha-santalene and (-)-beta-santalene, also (-)-exo-alpha-bergamotene, and low amounts of (-)-epi-beta-santalene from (2E,6E)-farnesyl diphosphate. The enzyme assay is coupled to the inorganic diphosphatase (IPP1) from Saccharomyces cerevisiae. Compound identification and quantification by GC-MS and NMR analysis
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
(2E,6E)-farnesyl diphosphate
(+)-alpha-santalene + diphosphate
-
-
-
?
(2E,6E)-farnesyl diphosphate
alpha-santalene + diphosphate
-
products are alpha-santalene, beta-, and epi-beta-santalene, exo-alpha-bergamotene, as well as traces of alpha- and beta-farnesene
-
?
additional information
?
-
Santalum album Ssy also accepts (2Z,6Z)-farnesyl diphosphate as substrate, producing a mixture of alpha-endo-bergamotene, alpha-santalene, (Z)-beta-farnesene, epi-beta-santalene, and beta-santalene. With substrate geranyl diphosphate, products are linalool, geraniol, and terpineol along with traces of alpha-pinene and camphene. The product profile resulting from incubations of SaSSy with (E,E)-FPP resembles the authentic essential oil of Santalum album more closely than that of the (Z,Z)-FPP incubations
-
-
?
additional information
?
-
the enzyme produces high amounts of (+)-alpha-santalene and (-)-beta-santalene, also (-)-exo-alpha-bergamotene, and low amounts of (-)-epi-beta-santalene from (2E,6E)-farnesyl diphosphate
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Mg2+
Mn2+
when incubated in the presence of 10 mM Mg2+ the enzyme converts (E,E)-farnesyl diphosphate into alpha-santalene, beta-santalene and epi-beta-santalene,alpha-exo-bergamotene, as well as traces of alpha-farnesene and beta-farnesene. Incubations with Mn2+ yields mainly alpha-exo-bergamotene
Mg2+
when incubated in the presence of 10 mM Mg2+ the enzyme converts (E,E)-farnesyl diphosphate into alpha-santalene, beta-santalene and epi-beta-santalene,alpha-exo-bergamotene, as well as traces of alpha-farnesene and beta-farnesene. Incubations with Mn2+ yields mainly alpha-exo-bergamotene
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
additional information
additional information
Michaelis-Menten kinetic analysis, overview. Kinetics are measured by IPP1 coupled colorimetric assay
-
0.0027
(2E,6E)-farnesyl diphosphate
recombinant His-tagged enzyme, pH 7.4, 37°C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.073
(2E,6E)-farnesyl diphosphate
recombinant His-tagged enzyme, pH 7.4, 37°C
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
27.04
(2E,6E)-farnesyl diphosphate
recombinant His-tagged enzyme, pH 7.4, 37°C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
pI VALUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
expression in mature outer and transition zone is higher by 10% as compared to immature wood sample
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
physiological function
the enzyme is involved in sandalwood oil biosynthesis
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). SASY_SANAL
569
0
65163
Swiss-Prot
other Location (Reliability: 2)
PDB
SCOP
CATH
UNIPROT
ORGANISM
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) ickel affinity chromatography, dialysis, and ultrafiltration to near homogeneity
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
DNA and amino acid sequence determination and analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Jones, C.G.; Moniodis, J.; Zulak, K.G.; Scaffidi, A.; Plummer, J.A.; Ghisalberti, E.L.; Barbour, E.L.; Bohlmann, J.
Sandalwood fragrance biosynthesis involves sesquiterpene synthases of both the terpene synthase (TPS)-a and TPS-b subfamilies, including santalene synthases
J. Biol. Chem.
286
17445-17454
2011
Santalum album (E3W202), Santalum austrocaledonicum (E3W203), Santalum spicatum (E3W204)
Rani, A.; Ravikumar, P.; Reddy, M.D.; Kush, A.
Molecular regulation of santalol biosynthesis in Santalum album L.
Gene
527
642-648
2013
Santalum album (K7WTQ7), Santalum album
Hua, G.; Hu, Y.; Yang, C.; Liu, D.; Mao, Z.; Zhang, L.; Zhang, Y.
Characterization of santalene synthases using an inorganic pyrophosphatase coupled colorimetric assay
Anal. Biochem.
547
26-36
2018
Clausena lansium (E5LLI1), Clausena lansium, Santalum album (E3W202), Santalum spicatum (E3W204)
EXTERNAL LINKS (specific for EC-Number 4.2.3.82)
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
