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Information on EC 4.2.3.81 - exo-alpha-bergamotene synthase and Organism(s) Lavandula angustifolia and UniProt Accession Q2XSC4

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EC Tree
     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.3 Acting on phosphates
                4.2.3.81 exo-alpha-bergamotene synthase
IUBMB Comments
The enzyme synthesizes a mixture of sesquiterpenoids from (2E,6E)-farnesyl diphosphate. As well as (-)-exo-alpha-bergamotene (74%) there were (E)-nerolidol (10%), (Z)-alpha-bisabolene (6%), (E)-beta-farnesene (5%) and beta-sesquiphellandrene (1%).
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This record set is specific for:
Lavandula angustifolia
UNIPROT: Q2XSC4
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Word Map
The taxonomic range for the selected organisms is: Lavandula angustifolia
The enzyme appears in selected viruses and cellular organisms
Synonyms
sassy, cotps2, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
PATHWAY SOURCE
PATHWAYS
-
-, -
SYSTEMATIC NAME
IUBMB Comments
(2E,6E)-farnesyl diphosphate lyase (cyclizing, (-)-exo-alpha-bergamotene-forming)
The enzyme synthesizes a mixture of sesquiterpenoids from (2E,6E)-farnesyl diphosphate. As well as (-)-exo-alpha-bergamotene (74%) there were (E)-nerolidol (10%), (Z)-alpha-bisabolene (6%), (E)-beta-farnesene (5%) and beta-sesquiphellandrene (1%).
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(2E,6E)-farnesyl diphosphate
(-)-exo-alpha-bergamotene + diphosphate
show the reaction diagram
-
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
(2E,6E)-farnesyl diphosphate
(-)-exo-alpha-bergamotene + diphosphate
show the reaction diagram
-
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mg2+
product formation is highest in the presence of the cofactor Mg2+ with product concentration peaking at 50 mM
Mn2+
the assays with Mn2+ shows highest activity at 0.05 mM with 49% of the maximum product level
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0047
(2E,6E)-farnesyl diphosphate
pH 7.5, 23°C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.033
(2E,6E)-farnesyl diphosphate
pH 7.5, 23°C
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.7
(2E,6E)-farnesyl diphosphate
pH 7.5, 23°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
LABER_LAVAN
538
0
62405
Swiss-Prot
other Location (Reliability: 2)
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
bovine serum albumin stabilizes
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Landmann, C.; Fink, B.; Festner, M.; Dregus, M.; Engel, K.H.; Schwab, W.
Cloning and functional characterization of three terpene synthases from lavender (Lavandula angustifolia)
Arch. Biochem. Biophys.
465
417-429
2007
Lavandula angustifolia (Q2XSC4)
Manually annotated by BRENDA team