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Information on EC 4.2.3.81 - exo-alpha-bergamotene synthase and Organism(s) Zea mays and UniProt Accession Q2NM15

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EC Tree
     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.3 Acting on phosphates
                4.2.3.81 exo-alpha-bergamotene synthase
IUBMB Comments
The enzyme synthesizes a mixture of sesquiterpenoids from (2E,6E)-farnesyl diphosphate. As well as (-)-exo-alpha-bergamotene (74%) there were (E)-nerolidol (10%), (Z)-alpha-bisabolene (6%), (E)-beta-farnesene (5%) and beta-sesquiphellandrene (1%).
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Zea mays
UNIPROT: Q2NM15
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Word Map
The taxonomic range for the selected organisms is: Zea mays
The enzyme appears in selected viruses and cellular organisms
Synonyms
sassy, cotps2, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
PATHWAY SOURCE
PATHWAYS
-
-, -
SYSTEMATIC NAME
IUBMB Comments
(2E,6E)-farnesyl diphosphate lyase (cyclizing, (-)-exo-alpha-bergamotene-forming)
The enzyme synthesizes a mixture of sesquiterpenoids from (2E,6E)-farnesyl diphosphate. As well as (-)-exo-alpha-bergamotene (74%) there were (E)-nerolidol (10%), (Z)-alpha-bisabolene (6%), (E)-beta-farnesene (5%) and beta-sesquiphellandrene (1%).
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(2E,6E)-farnesyl diphosphate
(-)-exo-alpha-bergamotene + diphosphate
show the reaction diagram
additional information
?
-
enzyme TPS10 forms (E)-alpha-bergamotene and (E)-beta-farnesene along with 13 minor sesquiterpenes in herbivore-damaged leaves
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
(2E,6E)-farnesyl diphosphate
(-)-exo-alpha-bergamotene + diphosphate
show the reaction diagram
additional information
?
-
enzyme TPS10 forms (E)-alpha-bergamotene and (E)-beta-farnesene along with 13 minor sesquiterpenes in herbivore-damaged leaves
-
-
?
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
transgenic maize plants overexpressing EREB58 show constitutive overaccumulation of TPS10 transcripts and also two major TPS10 products, (E)-beta-farnesene and (E)-alpha-bergamotene. By contrast, the contents of these two sesquiterpenes are reduced in EREB58-RNAi transgenic lines, in which the expression of TPS10 is suppressed
metabolism
the herbivore-induced sesquiterpenes of maize are mainly produced by two terpene synthases, TPS10 and TPS23, which convert the substrate farnesyl diphosphate (FPP) into various structurally diverse products
physiological function
enzyme TPS10 forms (E)-alpha-bergamotene and (E)-beta-farnesene along with 13 minor sesquiterpenes in herbivore-damaged leaves
additional information
the region from -300 to -200 of the maize TPS10 promoter is both necessary and sufficient for its inducibility by herbivory, the herbivore- and jasmonate-responsive AP2/ERF transcription factor EREB58 is a positive regulator of TPS10 expression and TPS10 volatile emission in maize, and plays a potential role in indirect defense against herbivores in maize, EREB58 binds directly to the GCC-box within the promoter fragment from -300 to -200 and promotes TPS10 expression, phylogenetic analysis, overview
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
FARS_MAIZE
533
0
61534
Swiss-Prot
other Location (Reliability: 3)
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
transgenic maize plants overexpressing EREB58 show constitutive overaccumulation of TPS10 transcripts and also two major TPS10 products, (E)-beta-farnesene and (E)-alpha-bergamotene
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
gene TPS10, identification of the region from -300 to -200 of the maize TPS10 promoter as both necessary and sufficient for its inducibility by herbivory through 5' deletion mapping in Arabidopsis thaliana. Gene EREB58 has a spatiotemporal expression pattern highly similar to that of TPS10
overexpression of tps10 in Arabidopsis thaliana results in plants emitting high quantities of TPS10 sesquiterpene products identical to that released by maize
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
expressed in response to herbivore attack and is regulated at the transcript level
jasmonate induction of gene TPS10. The region from -300 to -200 of the maize TPS10 promoter is both necessary and sufficient for its inducibility by herbivory, phylogenetic analysis identifies the herbivore- and jasmonate-responsive AP2/ERF transcription factor EREB58 as a candidate regulator of TPS10 in maize, EREB58 binds directly to the GCC-box within the promoter fragment from -300 to -200 and promotes TPS10 expression
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Schnee, C.; Kllner, T.G.; Held, M.; Turlings, T.C.; Gershenzon, J.; Degenhardt, J.
The products of a single maize sesquiterpene synthase form a volatile defense signal that attracts natural enemies of maize herbivores
Proc. Natl. Acad. Sci. USA
103
1129-1134
2006
Zea mays (Q2NM15)
Manually annotated by BRENDA team
Li, S.; Wang, H.; Li, F.; Chen, Z.; Li, X.; Zhu, L.; Wang, G.; Yu, J.; Huang, D.; Lang, Z.
The maize transcription factor EREB58 mediates the jasmonate-induced production of sesquiterpene volatiles
Plant J.
84
296-308
2015
Zea mays (Q2NM15)
Manually annotated by BRENDA team