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EC Tree
IUBMB Comments Initial cyclization gives germacrene C in an enzyme bound form which is not released to the medium.
The enzyme appears in viruses and cellular organisms
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(2E,6E)-farnesyl diphosphate = (+)-delta-selinene + diphosphate
(2E,6E)-farnesyl diphosphate = (+)-delta-selinene + diphosphate
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(2E,6E)-farnesyl diphosphate = (+)-delta-selinene + diphosphate
enzyme utilizes both cisoid and transoid-nerolidyl diphosphate as cyclization intermediates
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(2E,6E)-farnesyl-diphosphate diphosphate-lyase [(+)-delta-selinene-forming]
Initial cyclization gives germacrene C in an enzyme bound form which is not released to the medium.
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(2E,6E)-farnesyl diphosphate
(+)-delta-selinene + diphosphate
(2E,6E)-farnesyl diphosphate
(+)-delta-selinene + diphosphate
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main products are 12.3% delta-selinene, 17.2% germacrene A, 14.2% germacrene C, 33.2% germacrene B
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(2E,6E)-farnesyl diphosphate
(+)-delta-selinene + diphosphate
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main products are 25.3% delta-selinene, 17.4% (E,E)-germacrene B, 9.7% guaia-6,9-diene, plus 31 further products. Most of the germacrenes produced bear a trans-C2,C3-double bond, revealing the transoid nature of the nerolidyl diphosphate intermediate
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UniProt
brenda
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TPSD4_ABIGR
581
0
67968
Swiss-Prot
other Location (Reliability: 2 )
A0A4Y6GLQ2_9ROSI
126
0
14815
TrEMBL
other Location (Reliability: 3 )
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67600
X * 67600, calculated
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D331A
mutation results in main products 24.6% delta-amorphene, 12.9 beta-bisabolene, 22.0% germacrene A
D331E
mutation results in main products 16.6% beta-bisabolene, 29.3% germacrene A, 9.3% alpha-bisabolene
D475A
mutation results in main products 16.6% beta-bisabolene, 29.1% germacrene A, 8.8% alpha-bisabolene
D475E
mutation results in main products 13.4% siberene, 27.4% amorphene, 9.1% beta-bisabolene, 10.9% germacrene A
D475N
mutation results in main products 16.8% siberene, 34.7% amorphene, 8.2% beta-bisabolene, 6.8% germacrene A
D566N
mutation results in main products 20.6% beta-bisabolene, 29.1% germacrene A, 8.4% alpha-bisabolene
H16A
mutation results in main products 2.0% delta-selinene, 19.5% germacrene A, 33.7% germacrene C, 21.9% germacrene B
H17A
mutation results in main products 1.5% delta-selinene, 19.8% germacrene A, 34.0% germacrene C, 21.8% germacrene B
R11A
mutation results in main products 2.5% delta-selinene, 18.2% germacrene A, 33.2% germacrene C, 26.5% germacrene B
R12A
mutation results in main products 3.4% delta-selinene, 17.7% germacrene A, 35.8% germacrene C, 28.1% germacrene B
Y552F
mutation results in main products 9.1% dleta-selinene, 17.0% germacrene A, 22.9% germacrene C, 33.4% germacrene B
additional information
truncation at residue R11 results in main products 8.7% delta-selinene, 17.7% germacrene A, 21.8% germacrene C, 32.4% germacrene B. Truncation at residue H16 results in main products 1.8% delta-selinene, 17.0% germacrene A, 27.6% germacrene C, 19.7% germacrene B, 18.0% gamma-humulene. Truncation at residue W21 results in main products 23.4% beta-bisabolene, 24.4% germacrene A, 15.2% germacrene C, 4.7% germacrene B, 26.4% gamma-humulene
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expression in Escherichia coli
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Little, D.B.; Croteau, R.B.
Alteration of product formation by directed mutagenesis and truncation of the multiple-product sesquiterpene synthases delta-selinene synthase and gamma-humulene synthase
Arch. Biochem. Biophys.
402
120-135
2002
Abies grandis (O64404)
brenda
Steele, C.L.; Crock, J.; Bohlmann, J.; Croteau, R.
Sesquiterpene synthases from grand fir (Abies grandis). Comparison of constitutive and wound-induced activities, and cDNA isolation, characterization, and bacterial expression of delta-selinene synthase and gamma-humulene synthase
J. Biol. Chem.
273
2078-2089
1998
Abies grandis (O64404)
brenda
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History
Enzyme Nomenclature
4.2.3.76 (+)-delta-selinene synthase
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