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Information on EC 4.2.3.73 - valencene synthase and Organism(s) Vitis vinifera and UniProt Accession Q6Q3H2

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EC Tree
     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.3 Acting on phosphates
                4.2.3.73 valencene synthase
IUBMB Comments
The recombinant enzyme from Vitis vinifera gave 49.5% (+)-valencene and 35.5% (-)-7-epi-alpha-selinene. Initial cyclization gives (+)-germacrene A in an enzyme bound form which is not released to the medium.
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This record set is specific for:
Vitis vinifera
UNIPROT: Q6Q3H2
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Word Map
The taxonomic range for the selected organisms is: Vitis vinifera
The enzyme appears in selected viruses and cellular organisms
Synonyms
valencene synthase, (+)-valencene synthase, vvval, valcs, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
PATHWAY SOURCE
PATHWAYS
-
-
SYSTEMATIC NAME
IUBMB Comments
(2E,6E)-farnesyl-diphosphate diphosphate-lyase (valencene-forming)
The recombinant enzyme from Vitis vinifera gave 49.5% (+)-valencene and 35.5% (-)-7-epi-alpha-selinene. Initial cyclization gives (+)-germacrene A in an enzyme bound form which is not released to the medium.
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(2E,6E)-farnesyl diphosphate
(+)-valencene + diphosphate
show the reaction diagram
-
about 49%, plus 35.5% (-)-7-epi-alpha-selinene
-
?
farnesyl diphosphate
(+)-valencene + diphosphate
show the reaction diagram
-
plus 7-epi-alpha-selinene
-
?
(2E,6E)-farnesyl diphosphate
(+)-valencene + diphosphate
show the reaction diagram
-
49.5% (+)-valencene and 35.5% (-)-7-epi-alpha-selinene, plus minor amounts of alpha-selinene, epi-aristolochene, E-beta-caryophyllene, alpha-humulene
-
?
farnesyl diphosphate
(+)-valencene + diphosphate
show the reaction diagram
-
49.5% (+)-valencene and 35.5% (-)-7-epi-alpha-selinene, plus minor amounts of alpha-selinene, epi-aristolochene, E-beta-caryophyllene, alpha-humulene
-
?
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
farnesyl diphosphate
(+)-valencene + diphosphate
show the reaction diagram
-
49.5% (+)-valencene and 35.5% (-)-7-epi-alpha-selinene, plus minor amounts of alpha-selinene, epi-aristolochene, E-beta-caryophyllene, alpha-humulene
-
?
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.8
calculated
5.8
calculated
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
SwissProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
detection of VvVal transcripts coincides with the stabilization of acid levels in the fruits following a four-week period of rapid depletion
Manually annotated by BRENDA team
vegetative tissues of young leaves
Manually annotated by BRENDA team
pollen grain, during bloom
Manually annotated by BRENDA team
green stem
Manually annotated by BRENDA team
transcripts become detectable in September, almost two months after flowering, then increase and reach a maximum at the final sample date in early October when the fruit is at peak maturity
Manually annotated by BRENDA team
additional information
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
TPSVS_VITVI
556
0
64432
Swiss-Prot
other Location (Reliability: 5)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
64400
x * 64400, calculated
64400
x * 64400, calculated
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 64400, calculated
?
x * 64400, calculated
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
expression in Escherichia coli
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
transcript levels are low in open pre-anthesis flowers, flowers after anthesis, or at early onset of fruit development
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Luecker, J.; Bowen, P.; Bohlmann, J.
Vitis vinifera terpenoid cyclases: functional identification of two sesquiterpene synthase cDNAs encoding (+)-valencene synthase and (-)-germacrene D synthase and expression of mono- and sesquiterpene synthases in grapevine flowers and berries
Phytochemistry
65
2649-2659
2004
Vitis vinifera, Vitis vinifera (Q6Q3H2), Vitis vinifera cv. Gewuerztraminer
Manually annotated by BRENDA team
Martin, D.M.; Toub, O.; Chiang, A.; Lo, B.C.; Ohse, S.; Lund, S.T.; Bohlmann, J.
The bouquet of grapevine (Vitis vinifera L. cv. Cabernet Sauvignon) flowers arises from the biosynthesis of sesquiterpene volatiles in pollen grains
Proc. Natl. Acad. Sci. USA
106
7245-7250
2009
Vitis vinifera (Q6Q3H2), Vitis vinifera L. cv. Cabernet Sauvignon (Q6Q3H2)
Manually annotated by BRENDA team