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Information on EC 4.2.3.5 - chorismate synthase and Organism(s) Bacillus subtilis and UniProt Accession P31104

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EC Tree
     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.3 Acting on phosphates
                4.2.3.5 chorismate synthase
IUBMB Comments
Requires FMN. The reaction goes via a radical mechanism that involves reduced FMN and its semiquinone (FMNH(.)). Shikimate is numbered so that the double-bond is between C-1 and C-2, but some earlier papers numbered the ring in the reverse direction.
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This record set is specific for:
Bacillus subtilis
UNIPROT: P31104
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Word Map
The taxonomic range for the selected organisms is: Bacillus subtilis
The enzyme appears in selected viruses and cellular organisms
Synonyms
chorismate synthase, chorismate synthetase, vlaro2, 5-enolpyruvylshikimate-3-phosphate phospholyase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5-enolpyruvylshikimate-3-phosphate phospholyase
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chorismate synthase
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chorismate synthetase
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VEG216
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-
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Vegetative protein 216
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-
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
5-O-(1-carboxyvinyl)-3-phosphoshikimate = chorismate + phosphate
show the reaction diagram
combination with flavin reductase activity
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SYSTEMATIC NAME
IUBMB Comments
5-O-(1-carboxyvinyl)-3-phosphoshikimate phosphate-lyase (chorismate-forming)
Requires FMN. The reaction goes via a radical mechanism that involves reduced FMN and its semiquinone (FMNH(.)). Shikimate is numbered so that the double-bond is between C-1 and C-2, but some earlier papers numbered the ring in the reverse direction.
CAS REGISTRY NUMBER
COMMENTARY hide
9077-07-0
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
O5-(1-carboxyvinyl)-3-phosphoshikimate
chorismate + phosphate
show the reaction diagram
5-enolpyruvylshikimate 3-phosphate
chorismate + phosphate
show the reaction diagram
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?
additional information
?
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enzyme has flavin reductase activity
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?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
O5-(1-carboxyvinyl)-3-phosphoshikimate
chorismate + phosphate
show the reaction diagram
seventh enzyme in shikimate pathway
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?
5-enolpyruvylshikimate 3-phosphate
chorismate + phosphate
show the reaction diagram
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?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
FMNH2
absolute requirement, Km: 0.0125 mM
flavins
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required, reduced forms e.g. FMN, FAD
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FMN
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required
NADPH
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required
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
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pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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Uniprot
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
24000
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x * 24000, SDS-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
trimer
heterotrimer
additional information
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, 0.05 mM Tris-HCl, pH 7.5, 0.01 mM FMN, 10% glycerol, 3 mM mercaptoethanol, 4 months
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4°C, 2 weeks
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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
together with NADPH dependent flavin reductase
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in a chorismate synthase deficient Saccharomyces cerevisiae strain
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Hasan, N.; Nester, E.W.
Dehydroquinate synthase in Bacillus subtilis. An enzyme associated with chorismate synthase and flavin reductase
J. Biol. Chem.
253
4999-5004
1978
Bacillus subtilis
Manually annotated by BRENDA team
White, P.J.; Mousdale, D.M.; Coggins, J.R.
A simple anaerobic assay for chorismate synthase
Biochem. Soc. Trans.
15
144-145
1978
Bacillus subtilis, Escherichia coli, Neurospora crassa
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Manually annotated by BRENDA team
Hasan, N.; Nester, E.W.
Purification and properties of chorismate synthase from Bacillus subtilis
J. Biol. Chem.
253
4993-4998
1978
Bacillus subtilis
Manually annotated by BRENDA team
Macheroux, P.; Schmid, J.; Amrhein, N.; Schaller, A.
A unique reaction in a common pathway. Mechanism and function of chorismate synthase in the shikimate pathway
Planta
207
325-334
1999
Bacillus subtilis (P31104), Capnoides sempervirens (P27793), Escherichia coli, Euglena gracilis, Neurospora crassa (Q12640), Saccharomyces cerevisiae, Solanum lycopersicum, Staphylococcus aureus
Manually annotated by BRENDA team
Ehammer, H.; Rauch, G.; Prem, A.; Kappes, B.; Macheroux, P.
Conservation of NADPH utilization by chorismate synthase and its implications for the evolution of the shikimate pathway
Mol. Microbiol.
65
1249-1257
2007
Bacillus subtilis, Escherichia coli, Plasmodium falciparum, Solanum lycopersicum, Tetrahymena thermophila, Toxoplasma gondii, Vitis vinifera
Manually annotated by BRENDA team