show all | hide all No of entries

Information on EC 4.2.3.49 - (3R,6E)-nerolidol synthase and Organism(s) Zea mays and UniProt Accession Q84ZW8

for references in articles please use BRENDA:EC4.2.3.49
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.3 Acting on phosphates
                4.2.3.49 (3R,6E)-nerolidol synthase
IUBMB Comments
The enzyme catalyses a step in the formation of (3E)-4,8-dimethylnona-1,3,7-triene, a key signal molecule in induced plant defense mediated by the attraction of enemies of herbivores . Nerolidol is a naturally occurring sesquiterpene found in the essential oils of many types of plants.
Specify your search results
Select one or more organisms in this record: ?
This record set is specific for:
Zea mays
UNIPROT: Q84ZW8
The taxonomic range for the selected organisms is: Zea mays
The enzyme appears in selected viruses and cellular organisms
Synonyms
terpene synthase 1, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
terpene synthase 1
291414
-
TPS1
291414
-
SYSTEMATIC NAME
IUBMB Comments
(2E,6E)-farnesyl-diphosphate diphosphate-lyase [(3R,6E)-nerolidol-forming]
The enzyme catalyses a step in the formation of (3E)-4,8-dimethylnona-1,3,7-triene, a key signal molecule in induced plant defense mediated by the attraction of enemies of herbivores [1]. Nerolidol is a naturally occurring sesquiterpene found in the essential oils of many types of plants.
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(2E,6E)-farnesyl diphosphate + H2O
(3R,6E)-nerolidol + diphosphate
show the reaction diagram
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
(2E,6E)-farnesyl diphosphate + H2O
(3R,6E)-nerolidol + diphosphate
show the reaction diagram
herbivore-induced production of (3R,6E)-nerolidol, an intermediate in the formation of 4,8-dimethyl-1,3(E),7-nonatriene
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.001
(2E,6E)-farnesyl diphosphate
30°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 7.7
half-maximal activity at pH 6.0 and pH 7.7
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30 - 37
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
cultivar B73 and Delprim
UniProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
transcripts are detected in the leaf and sheath tissue of 2-week-old uninjured maize plants, but none is found in the roots. After 16 h of herbivory by Egyptian cotton leafworm larvae, transcript levels in the leaves of maize cv B73 are induced about 8-fold. The tps1 transcript levels in the maize cv Delprim are high even in uninjured plants and are only marginally elevated by herbivory. In older plants (3 months), moderate levels of transcript are present in leaves of both herbivore-treated and untreated plants of both cultivars. 16 hours after the initiation of herbivore damage, there is a significant elevation in transcript level
Manually annotated by BRENDA team
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
ACSS_MAIZE
590
0
67328
Swiss-Prot
Chloroplast (Reliability: 5)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
140000
dimer, recombinant enzyme, gel filtration
67400
71000
monomer, gel filtration
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
2 * 67400, calculated from sequence
monomer
1 * 67400, calculated from sequence
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-80°C, stable for at least 1 week
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
overexpression in Escherichia coli
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
transcripts are detected in the leaf and sheath tissue of 2-week-old uninjured maize plants, but none is found in the roots. After 16 h of herbivory by Egyptian cotton leafworm larvae, transcript levels in the leaves of maize cv B73 are induced about 8-fold. The tps1 transcript levels in the maize cv Delprim are high even in uninjured plants and are only marginally elevated by herbivory. In older plants (3 months), moderate levels of transcript are present in leaves of both herbivore-treated and untreated plants of both cultivars
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Schnee, C.; Köllner, T.G.; Gershenzon, J.; Degenhardt, J.
The maize gene terpene synthase 1 encodes a sesquiterpene synthase catalyzing the formation of (E)-beta-farnesene, (E)-nerolidol, and (E,E)-farnesol after herbivore damage
Plant Physiol.
130
2049-2060
2002
Zea mays (Q84ZW8), Zea mays
Manually annotated by BRENDA team
Select items on the left to see more content.