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Information on EC 4.2.3.44 - isopimara-7,15-diene synthase and Organism(s) Picea abies and UniProt Accession Q675L5

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EC Tree
     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.3 Acting on phosphates
                4.2.3.44 isopimara-7,15-diene synthase
IUBMB Comments
The enzyme only gave isopimara-7,15-diene.
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This record set is specific for:
Picea abies
UNIPROT: Q675L5
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The taxonomic range for the selected organisms is: Picea abies
The enzyme appears in selected viruses and cellular organisms
Synonyms
irksl6, isopimara-7,15-diene synthase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
diTPS-ISO
-
isopimara-7, 15-diene synthase
-
PaTPS-Iso
-
PaTPS-Iso
-
-
-
-
additional information
cf. EC 5.5.1.12
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
(+)-copalyl diphosphate = isopimara-7,15-diene + diphosphate
show the reaction diagram
proposed reaction scheme
SYSTEMATIC NAME
IUBMB Comments
copalyl diphosphate-lyase (isopimara-7,15-diene-forming)
The enzyme only gave isopimara-7,15-diene.
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(+)-copalyl diphosphate
isopimara-7,15-diene + diphosphate
show the reaction diagram
-
-
-
?
copalyl diphosphate
isopimara-7,15-diene + diphosphate
show the reaction diagram
proposed reaction scheme
-
-
?
additional information
?
-
the bifunctional diterpene synthase isopimara-7,15-diene synthase (diTPS-ISO) catalyzes the cyclization of geranylgeranyl diphosphate to (+)-copalyl diphosphate (cf. EC 5.5.1.12) and the formation of isopimara-7,15-diene from (+)-copalyl diphosphate. Compound identification and quantification by GC-MS analysis
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
(+)-copalyl diphosphate
isopimara-7,15-diene + diphosphate
show the reaction diagram
-
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mg2+
required
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.8
calculated from sequence
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
the bifunctional diterpene synthase, isopimara-7,15-diene synthase (diTPS-ISO) and a multifunctional cytochrome P450, CYP720B4 perform the biosynthesis of isopimaric acid. The diterpene synthase converts geranylgeranyl diphosphate (GGPP) to the tricyclic diterpene isopimara-7,15-diene (isopimaradiene) via formation of (+)-copalyl diphosphate. In three subsequent oxygenation reactions, the endoplasmic reticulum-associated enzyme CYP720B4 catalyzes the conversion of isopimaradiene into isopimaric acid, pathway overview
physiological function
the chloroplastidic bifunctional diterpene synthase, isopimara-7,15-diene synthase (diTPS-ISO) from Picea abies and a cytosolic multifunctional cytochrome P450 (CYP720B4, UniProt ID E5FA70) from Picea sitchensis, which is associated to the endoplasmic reticulum. The diterpene synthase diTPS-ISO also converts geranylgeranyl diphosphate (GGPP) to the tricyclic diterpene isopimara-7,15-diene (isopimaradiene)
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
TPSD2_PICAB
867
0
99203
Swiss-Prot
Chloroplast (Reliability: 3)
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
transient heterologous expression of the isopimaric acid pathway in Nicotiana benthamiana. Expressing DXS and GGPPs from Plectranthus barbatus in Nicotiana benthamiana are efficient in boosting diterpenoid levels compared to coexpression of the Nicotiana endogenous DXS and GGPPs genes, which have no effect on boosting diterpenoid level. Pigment analysis of infiltrated leaves, overview
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
functional expression of Picea abies isopimara-7,15-diene synthase (diTPS-ISO) in Nicotiana benthamiana leaves via Agrobacterium tumefaciens transfection method, coexpression with a multifunctional cytochrome P450 (CYP720B4) from Picea sitchensis, subcloning in Escherichia coli strain NEB10beta
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Martin, D.M.; Faeldt, J.; Bohlmann, J.
Functional characterization of nine Norway spruce TPS genes and evolution of gymnosperm terpene synthases of the TPS-d subfamily
Plant Physiol.
135
1908-1927
2004
Picea abies (Q675L5), Picea abies
Manually annotated by BRENDA team
Gnanasekaran, T.; Vavitsas, K.; Andersen-Ranberg, J.; Nielsen, A.Z.; Olsen, C.E.; Hamberger, B.; Jensen, P.E.
Heterologous expression of the isopimaric acid pathway in Nicotiana benthamiana and the effect of N-terminal modifications of the involved cytochrome P450 enzyme
J. Biol. Eng.
9
24
2015
Picea abies (Q675L5)
Manually annotated by BRENDA team