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Information on EC 4.2.3.35 - syn-pimara-7,15-diene synthase and Organism(s) Oryza sativa and UniProt Accession Q0JEZ8

for references in articles please use BRENDA:EC4.2.3.35
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EC Tree
     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.3 Acting on phosphates
                4.2.3.35 syn-pimara-7,15-diene synthase
IUBMB Comments
This enzyme is a class I terpene synthase . 9beta-Pimara-7,15-diene is a precursor of momilactones A and B, rice diterpenoid phytoalexins that are produced in response to attack (by a pathogen, elicitor or UV irradiation) and are involved in the defense mechanism of the plant. Momilactone B can also act as an allochemical, being constitutively produced in the root of the plant and secreted to the rhizosphere where it suppresses the growth of neighbouring plants and soil microorganisms .
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This record set is specific for:
Oryza sativa
UNIPROT: Q0JEZ8
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Word Map
  • 4.2.3.35
  • phytoalexins
  • diterpene
  • sativa
  • momilactones
  • diterpenoids
  • ent-copalyl
  • cyclization
  • gibberellin
  • geranylgeranyl
  • type-a
  • phytocassanes
  • elicitor
  • cyclases
The taxonomic range for the selected organisms is: Oryza sativa
The enzyme appears in selected viruses and cellular organisms
Synonyms
osks4, osdts2, syn-pimara-7,15-diene synthase, 9beta-pimara-7,15-diene synthase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
9beta-pimara-7,15-diene synthase
-
-
OsDTS2
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
cyclization
-
-
elimination of diphosphate
-
-
SYSTEMATIC NAME
IUBMB Comments
9alpha-copalyl-diphosphate diphosphate-lyase (9beta-pimara-7,15-diene-forming)
This enzyme is a class I terpene synthase [1]. 9beta-Pimara-7,15-diene is a precursor of momilactones A and B, rice diterpenoid phytoalexins that are produced in response to attack (by a pathogen, elicitor or UV irradiation) and are involved in the defense mechanism of the plant. Momilactone B can also act as an allochemical, being constitutively produced in the root of the plant and secreted to the rhizosphere where it suppresses the growth of neighbouring plants and soil microorganisms [1].
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
9alpha-copalyl diphosphate
9beta-pimara-7,15-diene + diphosphate
show the reaction diagram
9,10-syn-copalyl diphosphate
9beta-pimara-7,15-diene + diphosphate
show the reaction diagram
-
-
product identification via radio TLC, GC and GC/MS
-
?
9alpha-copalyl diphosphate
9beta-pimara-7,15-diene + diphosphate
show the reaction diagram
-
-
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
9alpha-copalyl diphosphate
9beta-pimara-7,15-diene + diphosphate
show the reaction diagram
the enzyme is involved in the biosynthesis of oryzalexins A-F, phytoalexin biosynthesis
-
-
?
9,10-syn-copalyl diphosphate
9beta-pimara-7,15-diene + diphosphate
show the reaction diagram
-
-
product identification via radio TLC, GC and GC/MS
-
?
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
SwissProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
cell culture
Manually annotated by BRENDA team
-
OsDTS2 mRNA in leaves is up-regulated by conditions that stimulate phytoalexin biosynthesis
Manually annotated by BRENDA team
-
OsDTS2 mRNA is constitutively expressed
Manually annotated by BRENDA team
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Mohan, R.S.; Yee, N.K.; Coates, R.M.; Ren, Y.Y.; Stamenkovic, P.; Mendez, I.; West, C.A.
Biosynthesis of cyclic diterpene hydrocarbons in rice cell suspensions: conversion of 9,10-syn-labda-8(17),13-dienyl diphosphate to 9beta-pimara-7,15-diene and stemar-13-ene
Arch. Biochem. Biophys.
330
33-47
1996
Oryza sativa
Manually annotated by BRENDA team
Otomo, K.; Kanno, Y.; Motegi, A.; Kenmoku, H.; Yamane, H.; Mitsuhashi, W.; Oikawa, H.; Toshima, H.; Itoh, H.; Matsuoka, M.; Sassa, T.; Toyomasu, T.
Diterpene cyclases responsible for the biosynthesis of phytoalexins, momilactones A, B, and oryzalexins A-F in rice
Biosci. Biotechnol. Biochem.
68
2001-2006
2004
Oryza sativa (Q0JEZ8), Oryza sativa
Manually annotated by BRENDA team
Wilderman, P.R.; Xu, M.; Jin, Y.; Coates, R.M.; Peters, R.J.
Identification of syn-pimara-7,15-diene synthase reveals functional clustering of terpene synthases involved in rice phytoalexin/allelochemical biosynthesis
Plant Physiol.
135
2098-2105
2004
Oryza sativa
Manually annotated by BRENDA team