Information on EC 4.2.3.35 - syn-pimara-7,15-diene synthase

for references in articles please use BRENDA:EC4.2.3.35
Word Map on EC 4.2.3.35
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:

The enzyme appears in viruses and cellular organisms

top print hide
EC NUMBER
COMMENTARY hide
4.2.3.35
-
top print hide
RECOMMENDED NAME
GeneOntology No.
syn-pimara-7,15-diene synthase
top print hide
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
9alpha-copalyl diphosphate = 9beta-pimara-7,15-diene + diphosphate
show the reaction diagram
-
-
-
-
top print hide
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
cyclization
elimination of diphosphate
top print hide
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
diterpene phytoalexins precursors biosynthesis
Diterpenoid biosynthesis
-
-
Biosynthesis of secondary metabolites
-
-
top print hide
SYSTEMATIC NAME
IUBMB Comments
9alpha-copalyl-diphosphate diphosphate-lyase (9beta-pimara-7,15-diene-forming)
This enzyme is a class I terpene synthase [1]. 9beta-Pimara-7,15-diene is a precursor of momilactones A and B, rice diterpenoid phytoalexins that are produced in response to attack (by a pathogen, elicitor or UV irradiation) and are involved in the defense mechanism of the plant. Momilactone B can also act as an allochemical, being constitutively produced in the root of the plant and secreted to the rhizosphere where it suppresses the growth of neighbouring plants and soil microorganisms [1].
top
top print hide
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
SwissProt
Manually annotated by BRENDA team
top print hide
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(+)-copalyl-diphosphate
9beta-isopimara-7,15-diene + diphosphate
show the reaction diagram
reaction intermediate is C8-sandaracopimaradienyl cation
-
-
?
9,10-syn-copalyl diphosphate
9beta-pimara-7,15-diene + diphosphate
show the reaction diagram
-
-
product identification via radio TLC, GC and GC/MS
-
?
9alpha-copalyl diphosphate
9beta-pimara-7,15-diene + diphosphate
show the reaction diagram
top print hide
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
9,10-syn-copalyl diphosphate
9beta-pimara-7,15-diene + diphosphate
show the reaction diagram
-
-
product identification via radio TLC, GC and GC/MS
-
?
9alpha-copalyl diphosphate
9beta-pimara-7,15-diene + diphosphate
show the reaction diagram
Q0JEZ8
the enzyme is involved in the biosynthesis of oryzalexins A-F, phytoalexin biosynthesis
-
-
?
top print hide
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
cell culture
Manually annotated by BRENDA team
-
OsDTS2 mRNA in leaves is up-regulated by conditions that stimulate phytoalexin biosynthesis
Manually annotated by BRENDA team
-
OsDTS2 mRNA is constitutively expressed
Manually annotated by BRENDA team
top print hide
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
homology model of the second active site of enzyme based on the structure of 5-epiaristolochene synthase from Nicotiana tabacum, Protein Data Bank ID code 5EAT
top print hide
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
top print hide
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
A713S
products are isopimaradiene and sandaracopimaradiene
G651V
no change in product
V717L
no change in product
W679L/Y686H/A713S/V717L
products are isopimaradiene and sandaracopimaradiene
Y686H
no change in product
Y686H/A713S
main products are isopimaradiene and sandaracopimaradiene
Y686H/A713S A713S
main products are isopimaradiene and sandaracopimaradiene
Y686H/A713S/V717L
products are isopimaradiene and sandaracopimaradiene
additional information
swapping of residues 568�640 of isopimaradiene synhase to corresponding residues 560-632 of levopimaradiene/abietadiene synthase results in complete reversion of the product profiles of the two enzymes
top
top
top
LINKS TO OTHER DATABASES (specific for EC-Number 4.2.3.35)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
InterPro (database of protein families, domains and functional sites)