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Information on EC 4.2.3.33 - stemar-13-ene synthase and Organism(s) Oryza sativa and UniProt Accession Q6BDZ9

for references in articles please use BRENDA:EC4.2.3.33
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EC Tree
     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.3 Acting on phosphates
                4.2.3.33 stemar-13-ene synthase
IUBMB Comments
This diterpene cyclase produces stemar-13-ene, a putative precursor of the rice phytoalexin oryzalexin S. Phytoalexins are diterpenoid secondary metabolites that are involved in the defense mechanism of the plant, and are produced in response to pathogen attack through the perception of elicitor signal molecules such as chitin oligosaccharide, or after exposure to UV irradiation.
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This record set is specific for:
Oryza sativa
UNIPROT: Q6BDZ9
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Word Map
  • 4.2.3.33
  • phytoalexins
  • diterpene
  • oryzalexins
  • diterpenoids
  • oryza
  • sativa
  • syn-copalyl
  • momilactones
  • phytocassanes
  • gibberellin
  • oscps4
  • labdane-related
  • phytohormone
  • elicitors
  • chitin
  • suspension-cultured
  • geranylgeranyl
  • indica
  • ent-copalyl
The taxonomic range for the selected organisms is: Oryza sativa
The expected taxonomic range for this enzyme is: Oryza
Synonyms
osdtc2, osksl8, stemar-13-ene synthase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
OsDTC2
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-
stemar-13-ene synthase
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-
SYSTEMATIC NAME
IUBMB Comments
9alpha-copalyl-diphosphate diphosphate-lyase (stemar-13-ene-forming)
This diterpene cyclase produces stemar-13-ene, a putative precursor of the rice phytoalexin oryzalexin S. Phytoalexins are diterpenoid secondary metabolites that are involved in the defense mechanism of the plant, and are produced in response to pathogen attack through the perception of elicitor signal molecules such as chitin oligosaccharide, or after exposure to UV irradiation.
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
9alpha-copalyl diphosphate
stemar-13-ene + diphosphate
show the reaction diagram
9alpha-copalyl diphosphate
stemar-13-ene + diphosphate
show the reaction diagram
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
9alpha-copalyl diphosphate
stemar-13-ene + diphosphate
show the reaction diagram
the enzyme converts syn-copalyl diphosphate to stemar-13-ene, a putative diterpene hydrocarbon precursor of the phytoalexin oryzalexin S
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-
?
9alpha-copalyl diphosphate
stemar-13-ene + diphosphate
show the reaction diagram
-
the enzyme converts syn-copalyl diphosphate into stemar-13-ene, a putative diterpene hydrocarbon precursor of the phytoalexin oryzalexin S. The transcriptional expression of OsDTC2 is induced by treatment of suspension-cultured rice cells with a chitin oligosaccharide elicitor
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-
?
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
SwissProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
the level of OsDTC2 mRNA in suspension-cultured rice cells began to increase 3 h after addition of the elicitor and reached the maximum after 8 h
Manually annotated by BRENDA team
expression of OsDTC2 is induced in UV-irradiated rice leaves
Manually annotated by BRENDA team
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
OsDTC2 cDNA is overexpressed in Escherichia coli as a fusion protein with glutathione S-transferase
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Mohan, R.S.; Yee, N.K.; Coates, R.M.; Ren, Y.Y.; Stamenkovic, P.; Mendez, I.; West, C.A.
Biosynthesis of cyclic diterpene hydrocarbons in rice cell suspensions: conversion of 9,10-syn-labda-8(17),13-dienyl diphosphate to 9beta-pimara-7,15-diene and stemar-13-ene
Arch. Biochem. Biophys.
330
33-47
1996
Oryza sativa
Manually annotated by BRENDA team
Nemoto, T.; Okada, A.; Okada, K.; Shibuya, N.; Toyomasu, T.; Nojiri, H.; Yamane, H.
Promoter analysis of the rice stemar-13-ene synthase gene OsDTC2, which is involved in the biosynthesis of the phytoalexin oryzalexin S
Biochim. Biophys. Acta
1769
678-683
2007
Oryza sativa
Manually annotated by BRENDA team
Nemoto, T.; Cho, E.M.; Okada, A.; Okada, K.; Otomo, K.; Kanno, Y.; Toyomasu, T.; Mitsuhashi, W.; Sassa, T.; Minami, E.; Shibuya, N.; Nishiyama, M.; Nojiri, H.; Yamane, H.
Stemar-13-ene synthase, a diterpene cyclase involved in the biosynthesis of the phytoalexin oryzalexin S in rice
FEBS Lett.
571
182-186
2004
Oryza sativa (Q6BDZ9), Oryza sativa
Manually annotated by BRENDA team