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Information on EC 4.2.3.32 - levopimaradiene synthase and Organism(s) Pinus taeda and UniProt Accession Q50EK2

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EC Tree
     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.3 Acting on phosphates
                4.2.3.32 levopimaradiene synthase
IUBMB Comments
In Ginkgo, the enzyme catalyses the initial cyclization step in the biosynthesis of ginkgolides, a structurally unique family of diterpenoids that are highly specific platelet-activating-factor receptor antagonists . Levopimaradiene is widely distributed in higher plants. In some species the enzyme also forms abietadiene, palustradiene, and neoabietadiene .
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This record set is specific for:
Pinus taeda
UNIPROT: Q50EK2
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Word Map
The taxonomic range for the selected organisms is: Pinus taeda
The enzyme appears in selected viruses and cellular organisms
Synonyms
levopimaradiene/abietadiene synthase, levopimaradiene synthase, lp synthase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SYSTEMATIC NAME
IUBMB Comments
(+)-copalyl-diphosphate diphosphate-lyase [abieta-8(14),12-diene-forming]
In Ginkgo, the enzyme catalyses the initial cyclization step in the biosynthesis of ginkgolides, a structurally unique family of diterpenoids that are highly specific platelet-activating-factor receptor antagonists [1]. Levopimaradiene is widely distributed in higher plants. In some species the enzyme also forms abietadiene, palustradiene, and neoabietadiene [2].
CAS REGISTRY NUMBER
COMMENTARY hide
369596-13-4
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(+)-copalyl-diphosphate
(-)-abietadiene + levopimaradiene + neoabietadiene + palustradiene + diphosphate
show the reaction diagram
reaction intermediate is C8-sandaracopimaradienyl cation
bifunctional levopimaradiene/abietadiene synthase, about 47% levopimaradiene, 27% abietadiene, 22% neoabietadiene, 3% palustradiene
-
?
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
SwissProt
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
TPSD1_PINTA
850
0
97499
Swiss-Prot
Chloroplast (Reliability: 3)
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression of green fluorescent fusion protein in tobacco cells
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Ro, D.K.; Bohlmann, J.
Diterpene resin acid biosynthesis in loblolly pine (Pinus taeda): Functional characterization of abietadiene/levopimaradiene synthase (PtTPS-LAS) cDNA and subcellular targeting of PtTPS-LAS and abietadienol/abietadienal oxidase (PtAO, CYP720B1)
Phytochemistry
67
1572-1578
2006
Pinus taeda (Q50EK2), Pinus taeda
Manually annotated by BRENDA team