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IUBMB CommentsThis enzyme is involved in the biosynthesis of the diterpenoid viguiepinol and requires Mg2+, Co2+, Zn2+ or Ni2+ for activity.
The expected taxonomic range for this enzyme is: unclassified Streptomyces
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ent-copalyl diphosphate = ent-pimara-9(11),15-diene + diphosphate
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ent-copalyl-diphosphate diphosphate-lyase [ent-pimara-9(11),15-diene-forming]
This enzyme is involved in the biosynthesis of the diterpenoid viguiepinol and requires Mg2+, Co2+, Zn2+ or Ni2+ for activity.
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ent-copalyl diphosphate
pimara-9(11),15-diene + diphosphate
additional information
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ent-copalyl diphosphate
pimara-9(11),15-diene + diphosphate
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Substrates: -
Products: -
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ent-copalyl diphosphate
pimara-9(11),15-diene + diphosphate
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Substrates: -
Products: -
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additional information
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Substrates: no substrates: farnesyl diphosphate, geranylgeranyl diphosphate, terpentecin diphosphate
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additional information
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Substrates: no substrates: farnesyl diphosphate, geranylgeranyl diphosphate, terpentecin diphosphate
Products: -
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Co2+
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1 mM, 100% of the activity with Mg2+
Mg2+
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absolutely required, optimal concentraion 1 mM
Ni2+
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1 mM, 57% of the activity with Mg2+
Zn2+
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1 mM, 59% of the activity with Mg2+
additional information
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no or very weak activity in presence of Mn2+, Ca2+, Cu2+ and Fe2+
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2-mercaptoethanol
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5 mM, required for full activity
Tween-80
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0.1%, required for full activity
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0.00026
ent-copalyl diphosphate
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30°C, pH 7.0
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0.0014
ent-copalyl diphosphate
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30°C, pH 7.0
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brenda
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brenda
Highest Expressing Human Cell Lines
Cell Line Links
Gene Links
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PMDS_STREO
Streptomyces sp. (strain KO-3988)
295
0
32795
Swiss-Prot
other Location (Reliability: 1)
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monomer
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x * 35000, SDS-PAGE
monomer
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x * 35000, SDS-PAGE
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35
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pH 5.5, stable for at least 1 h
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Ikeda, C.; Hayashi, Y.; Itoh, N.; Seto, H.; Dairi, T.
Functional analysis of eubacterial ent-copalyl diphosphate synthase and pimara-9(11),15-diene synthase with unique primary sequences
J. Biochem.
141
37-45
2007
Streptomyces sp., Streptomyces sp. KO-3988
brenda
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