Substrates: mechanism involves an initial rapid equilibrium of enzyme with substrate to form an enzyme-substrate complex, followed by a slower conversion of farnesyl diphosphate to an enzyme-bound hydrocarbon, and a subsequent rate-limiting step Products: -
Substrates: infection of Phytophthora infestans with potato tubers causes transient increase in the transcript level of vetispiradiene synthase. Wound-induced expression of the squalene synthase is suppressed in favor of the expression of vetispiradiene synthase Products: -
Substrates: infection of Phytophthora infestans with potato tubers causes transient increase in the transcript level of vetispiradiene synthase. Wound-induced expression of the squalene synthase is suppressed in favor of the expression of vetispiradiene synthase Products: -
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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant vetispiradiene synthase, a chimeric 5-epi-aristolochene synthase and a chimeric sesquiterpene cyclase possessing multifunctional epi-aristolochene and vetispiradiene activity
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli. Highest level of sesquiterpene production occurs when the enzymes are expressed in strain DH5alpha from the trc promoter of the high-copy plasmid pTrc99A in M9 medium supplemented with 0.2% v/v glycerol at 30°C
hexahistidyl-tagged enzyme expressed in Escherichia coli, genes for vetispiradiene synthase, a chimeric 5-epi-aristolochene synthase and a chimeric sesquiterpene cyclase possessing multifunctional epi-aristolochene and vetispiradiene activity
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
after 24 h incubation in the dark, the gene coding for vetispiradiene synthase pvs1 is significantly induced incultured varieties Favorita and Longshu-3. The genes coding for hydroxymethylglutaryl coenzyme A reductase and squalene synthase remain relatively stable in wild species Solanum chacoense and variety Longshu-3
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