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Information on EC 4.2.3.199 - (-)-5-epieremophilene synthase
for references in articles please use BRENDA:EC4.2.3.199
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EC Tree 4 Lyases
4.2 Carbon-oxygen lyases
4.2.3 Acting on phosphates
4.2.3.199 (-)-5-epieremophilene synthase
IUBMB Comments
The plant Salvia miltiorrhiza (danshen) produces three different forms of the enzyme, encoded by paralogous genes, that exhibit different spacial expression patterns and respond differently to hormone treatment.
The expected taxonomic range for this enzyme is: Salvia miltiorrhiza
showSynonyms
(-)-5-epieremophilene synthase, more
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
STP2
STP3
STPS1
STP2
-
-
-
-
STP3
-
-
-
-
STPS1
-
-
-
-
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
(2E,6E)-farnesyl diphosphate = (-)-5-epieremophilene + diphosphate
-
-
-
-
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SYSTEMATIC NAME
IUBMB Comments
(2E,6E)-farnesyl-diphosphate diphosphate-lyase [cyclizing, (-)-epieremophilene-forming]
The plant Salvia miltiorrhiza (danshen) produces three different forms of the enzyme, encoded by paralogous genes, that exhibit different spacial expression patterns and respond differently to hormone treatment.
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
(2E,6E)-farnesyl diphosphate
(-)-5-epieremophilene + diphosphate
Substrates: -
Products: -
Products: -
?
(2E,6E)-farnesyl-diphosphate
(-)-5-epieremophilene + diphosphate
Substrates: -
Products: -
Products: -
?
(2E,6E)-farnesyl-diphosphate
(-)-5-epieremophilene + diphosphate
Substrates: (-)-5-epieremophilene i.e. (3R,4aS,5S)-4a,5-dimethyl-3-(prop-1-en-2-yl)-1,2,3,4,4a,5,6,7-octahydronaphthalene. Salvia miltiorrhiza produces three different forms of the enzyme, encoded by paralogous genes, that exhibit different spacial expression patterns and respond differently to hormone treatment (STPS1, STP2m and STP3)
Products: -
Products: -
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
(2E,6E)-farnesyl-diphosphate
(-)-5-epieremophilene + diphosphate
Substrates: -
Products: -
Products: -
?
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.01044
(2E,6E)-farnesyl-diphosphate
30°C, pH 7.0, enzyme form SmSTPS3
0.0242
(2E,6E)-farnesyl-diphosphate
30°C, pH 7.0, enzyme form SmSTPS2
0.02535
(2E,6E)-farnesyl-diphosphate
30°C, pH 7.0, enzyme form SmSTPS1
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
1.53
(2E,6E)-farnesyl-diphosphate
30°C, pH 7.0, enzyme form SmSTPS3
2.07
(2E,6E)-farnesyl-diphosphate
30°C, pH 7.0, enzyme form SmSTPS2
2.09
(2E,6E)-farnesyl-diphosphate
30°C, pH 7.0, enzyme form SmSTPS1
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kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
82.7
(2E,6E)-farnesyl-diphosphate
30°C, pH 7.0, enzyme form SmSTPS1
85.6
(2E,6E)-farnesyl-diphosphate
30°C, pH 7.0, enzyme form SmSTPS2
148
(2E,6E)-farnesyl-diphosphate
30°C, pH 7.0, enzyme form SmSTPS3
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
mainly expressed in leaf and inflorescence, transcript level is low or undetectable in stem and root
brenda
mainly expressed in leaf and inflorescence, transcript level is low or undetectable in stem and root. SmSTPS2 is weakly expressed
brenda
mainly expressed in leaf and inflorescence, transcript level is low or undetectable in stem and root. SmSTPS3 is expressed predominantly in inflorescence
brenda
mainly expressed in leaf and inflorescence, transcript level is low or undetectable in stem and root. SmSTPS1 is expressed at a higher level than the SmSTPS2 and SmSTPS3, and its transcripts are most abundant in leaf
brenda
mainly expressed in leaf and inflorescence, transcript level is low or undetectable in stem and root. SmSTPS2 is weakly expressed and exhibits a clear expression only in leaf
brenda
mainly expressed in leaf and inflorescence, transcript level is low or undetectable in stem and root
brenda
mainly expressed in leaf and inflorescence, transcript level is low or undetectable in stem and root
brenda
mainly expressed in leaf and inflorescence, transcript level is low or undetectable in stem and root. SmSTPS2 is weakly expressed
brenda
mainly expressed in leaf and inflorescence, transcript level is low or undetectable in stem and root
brenda
mainly expressed in leaf and inflorescence, transcript level is low or undetectable in stem and root. SmSTPS2 is weakly expressed
brenda
Highest Expressing Human Cell Lines
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GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
physiological function
(-)-5-epieremophilene shows significant antifeedant activity against Helicoverpa armigera (EC50 is 1.25 microg/cm2), a common pest of Salvia miltiorrhiza
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UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). STPS3_SALMI
546
0
63501
Swiss-Prot
other Location (Reliability: 1)
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SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli Rosetta (DE3)
transient expression in Nicotiana benthamiana
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
SmSTPS1 is induced strongly by ethylene and moderately by methyl jasmonate, abscicic acid and gibberellin A3
SmSTPS2 is induced by ethylene or by abscicic acid
SmSTPS3 is up-regulated slightly only by abscicic acid
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
synthesis
synthesis of (-)-5-epieremophilene in engineered Escherichia coli harboring a heterologous methylvalonate pathway and FDP synthase yields up to 76 mg/l (-)-5-epieremophilene. Expression of a fusion protein FDP synthase-STPS1 increases the yield, and segmentation of the FDP biosynthetic pathway into two modules further improves production to 68.6 mg/l
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Fang, X.; Li, C.Y.; Yang, Y.; Cui, M.Y.; Chen, X.Y.; Yang, L.
Identification of a novel (-)-5-epieremophilene synthase from Salvia miltiorrhiza via transcriptome mining
Front. Plant Sci.
8
627
2017
Salvia miltiorrhiza (A0A1W6GW32), Salvia miltiorrhiza (A0A1W6GW06), Salvia miltiorrhiza (A0A1W6GW18), Salvia miltiorrhiza
brenda
Luo, L.Q.; Chen, Y.G.; Li, D.S.; Liu, Y.; Li, S.H.
Production of the inaccessible sesquiterpene (-)-5-epieremophilene by metabolically engineered Escherichia coli
Chem. Biodivers.
17
e2000219
2020
Salvia miltiorrhiza (A0A1W6GW32)
brenda
EXTERNAL LINKS (specific for EC-Number 4.2.3.199)
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Release 2026.1 (March 2026)
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