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Information on EC 4.2.3.189 - 9,13-epoxylabda-14-ene synthase and Organism(s) Marrubium vulgare and UniProt Accession A0A075FBG7

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EC Tree
     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.3 Acting on phosphates
                4.2.3.189 9,13-epoxylabda-14-ene synthase
IUBMB Comments
Isolated from the plants Marrubium vulgare (white horehound) and Vitex agnus-castus (chaste tree). Involved in marrubiin biosynthesis.
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This record set is specific for:
Marrubium vulgare
UNIPROT: A0A075FBG7
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The taxonomic range for the selected organisms is: Marrubium vulgare
The enzyme appears in selected viruses and cellular organisms
Synonyms
mvels, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
MvELS
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
peregrinol-diphosphate diphosphate-lyase (9,13-epoxylabda-14-ene-forming)
Isolated from the plants Marrubium vulgare (white horehound) and Vitex agnus-castus (chaste tree). Involved in marrubiin biosynthesis.
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
peregrinol diphosphate
(13epsilon)-9,13-epoxylabda-14-ene + diphosphate
show the reaction diagram
-
-
-
?
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
peregrinol diphosphate
(13epsilon)-9,13-epoxylabda-14-ene + diphosphate
show the reaction diagram
-
-
-
?
additional information
?
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
transcript level is 4fold higher in leaf compared with flower
Manually annotated by BRENDA team
transcript level is 4fold higher in leaf compared with flower
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
in vitro, in combination with the copalyl diphosphate-producing CPS3, ELS forms miltiradiene. When coupled with TPS2, ELS converts (13E)-8alpha-hydroxylabd-13-en-15-yl diphosphate into manoyl oxide. The coupled reaction of CPS1 and ELS leads to 80% (13epsilon)-9,13-epoxylabda-14-ene
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
ELS_MARVU
580
0
67098
Swiss-Prot
Chloroplast (Reliability: 4)
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
ELS belongs to a subgroup of the diTPS TPS–e/f clade with unusual betaalpha-domain architecture
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Zerbe, P.; Chiang, A.; Dullat, H.; ONeil-Johnson, M.; Starks, C.; Hamberger, B.; Bohlmann, J.
Diterpene synthases of the biosynthetic system of medicinally active diterpenoids in Marrubium vulgare
Plant J.
79
914-927
2014
Marrubium vulgare (A0A075FBG7), Marrubium vulgare
Manually annotated by BRENDA team