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Synonyms
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sesterterpene synthase
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(2E,6E,10E,14E)-geranylfarnesyl diphosphate = stellata-2,6,19-triene + diphosphate
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MetaCyc
stellatic acid biosynthesis
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(2E,6E,10E,14E)-geranylfarnesyl-diphosphate diphosphate-lyase (cylizing, stellata-2,6,19-triene-forming)
Isolated from the fungus Aspergillus stellatus.
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(2E,6E,10E,14E)-geranylfarnesyl diphosphate
stellata-2,6,19-triene + diphosphate
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Substrates: -
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(2E,6E,10E,14E)-geranylfarnesyl diphosphate
stellata-2,6,19-triene + diphosphate
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Substrates: -
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Mg2+
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the DDXXD motif for binding a trinuclear Mg2+ cluster is conserved in both the N-terminal terpene cyclase and C-terminal prenyl tranferase domains as 92DDVTD96 and 473DDVED477, respectively. Assay in presence of Mg2+
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Highest Expressing Human Cell Lines
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physiological function
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the enzyme synthesizes the C25 linear substrate (2E,6E,10E,14E)-geranylfarnesyl diphosphate from dimethylallyl diphosphate and 4 molecules of isopentenyl dipohosphate and also cyclizes (2E,6E,10E,14E)-geranylfarnesyl diphosphate into stellata-2,6,19-triene, which is further transformed into stellatic acid by a cytochrome P450 monooxygenase encoded by the gene adjacent to the sesterterpene synthase gene
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STLSS_EMEVA
714
0
80706
Swiss-Prot
other Location (Reliability: 5 )
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expression in Aspergillus oryzae and Escherichia coli
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Matsuda, Y.; Mitsuhashi, T.; Quan, Z.; Abe, I.
Molecular basis for stellatic acid biosynthesis a genome mining approach for discovery of sesterterpene synthases
Org. Lett.
17
4644-4647
2015
Aspergillus stellatus
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