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Information on EC 4.2.3.154 - demethyl-4-deoxygadusol synthase and Organism(s) Trichormus variabilis and UniProt Accession Q3M6C3

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EC Tree
     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.3 Acting on phosphates
                4.2.3.154 demethyl-4-deoxygadusol synthase
IUBMB Comments
The enzyme, characterized from the cyanobacterium Nostoc punctiforme PCC 73102, is involved in the biosynthesis of the sunscreen compound shinorine. It requires a divalent metal ion (Zn2+ or Co2+) and an NAD+ cofactor, which is transiently reduced during the reaction. cf. EC 4.2.3.152, 2-epi-5-epi-valiolone synthase and EC 4.2.3.155, 2-epi-valiolone synthase.
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This record set is specific for:
Trichormus variabilis
UNIPROT: Q3M6C3
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The taxonomic range for the selected organisms is: Trichormus variabilis
The enzyme appears in selected viruses and cellular organisms
Synonyms
desmethyl-4-deoxygadusol synthase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
desmethyl-4-deoxygadusol synthase
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PATHWAY SOURCE
PATHWAYS
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-
SYSTEMATIC NAME
IUBMB Comments
D-sedoheptulose-7-phosphate phosphate-lyase (cyclizing; demethyl-4-deoxygadusol-forming)
The enzyme, characterized from the cyanobacterium Nostoc punctiforme PCC 73102, is involved in the biosynthesis of the sunscreen compound shinorine. It requires a divalent metal ion (Zn2+ or Co2+) and an NAD+ cofactor, which is transiently reduced during the reaction. cf. EC 4.2.3.152, 2-epi-5-epi-valiolone synthase and EC 4.2.3.155, 2-epi-valiolone synthase.
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
D-sedoheptulose 7-phosphate
demethyl-4-deoxygadusol + phosphate + H2O
show the reaction diagram
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
D-sedoheptulose 7-phosphate
demethyl-4-deoxygadusol + phosphate + H2O
show the reaction diagram
the enzyme is involved in the biosynthesis of the sunscreen compound shinorine
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-
?
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0216
D-sedoheptulose 7-phosphate
pH 7.4, 25°C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0533
D-sedoheptulose 7-phosphate
pH 7.4, 25°C
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2.47
D-sedoheptulose 7-phosphate
pH 7.4, 25°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
SwissProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
the enzyme is involved in the biosynthesis of the sunscreen compound shinorine
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Asamizu, S.; Xie, P.; Brumsted, C.J.; Flatt, P.M.; Mahmud, T.
Evolutionary divergence of sedoheptulose 7-phosphate cyclases leads to several distinct cyclic products
J. Am. Chem. Soc.
134
12219-12229
2012
Nostoc punctiforme, Trichormus variabilis (Q3M6C3)
Manually annotated by BRENDA team