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Information on EC 4.2.3.15 - myrcene synthase for references in articles please use BRENDA:EC4.2.3.15Word Map on EC 4.2.3.15
4.2.3.15
terpene
monoterpene
volatile
limonene
sesquiterpene
emit
terpenoids
blend
acyclic
lepidopteran
enemies
herbivory
herbivore-induced
gymnosperm
linalool
scent
olefins
jasmonate-induced
e-beta-ocimene
grandis
parasitoid
quercus
pinene
neryl
e-beta-farnesene
tepals
cotesia
headspace
olfactometer
conifer
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The enzyme appears in viruses and cellular organisms
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geranyl diphosphate = myrcene + diphosphate
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elimination, C-O bond cleavage
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ipsdienol biosynthesis
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monoterpene biosynthesis
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oleoresin monoterpene volatiles biosynthesis
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Monoterpenoid biosynthesis
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Biosynthesis of secondary metabolites
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geranyl-diphosphate diphosphate-lyase (myrcene-forming)
A recombinant enzyme (also known as a monoterpene synthase or cyclase) from the grand fir (Abies grandis) requires Mn2+ and K+ for activity. Mg2+ is essentially ineffective as the divalent metal ion cofactor.
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myrcene/(E)-beta-ocimene synthase
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grand fir
Uniprot
brenda
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brenda
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brenda
; the tandem arginine motif RRX8W is conserved in the deduced protein of TPS10; bifunctional myrcene/(E)-beta-ocimene synthase
SwissProt
brenda
bifunctional enzyme: geranyldiphosphate synthase/myrcene synthase
SwissProt
brenda
marine red alga
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brenda
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brenda
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geranyl diphosphate
(+)-limonene
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less than 5% of total hydrocarbon product
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geranyl diphosphate
(-)-limonene
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less than 5% of total hydrocarbon product
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geranyl diphosphate
(E)-beta-ocimene
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20% of total hydrocarbon product
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geranyl diphosphate
2-carene
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less than 5% of total hydrocarbon product
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geranyl diphosphate
myrcene + diphosphate
geranyl diphosphate
myrcene + sabinene + linalool + limonene + diphosphate
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formation of 53.8% myrcene, 20.9% sabinene, 19.8% linalool and 5.5% limonene
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geranyl diphosphate
tricyclene
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less than 5% of total hydrocarbon product
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linalyl diphosphate
acyclic and cyclic monoterpenes
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neryl diphosphate
limonene
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additional information
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terpenes produced from the wild-type and mutant enzymes are identical, showing low levels of beta-myrcene from AcTPS2-K514A
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geranyl diphosphate
myrcene + diphosphate
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geranyl diphosphate
myrcene + diphosphate
induced by stem wounding, enhances resistance to insects
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geranyl diphosphate
myrcene + diphosphate
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geranyl diphosphate
myrcene + diphosphate
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geranyl diphosphate
myrcene + diphosphate
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56% of total hydrocarbon product
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geranyl diphosphate
myrcene + diphosphate
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products of Arabidopsis thaliana TPS10 are 56% beta-myrcene, 20% (E)-beta-ocimene, and less than 5% each of (+)-limonene, (-)-limonene, 2-carene, and tricyclene and an unknown monoterpene
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geranyl diphosphate
myrcene + diphosphate
the acyclic monoterpene myrcene is required for the production of the major aggregation pheromone component, ipsdienol
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geranyl diphosphate
myrcene + diphosphate
bifunctional enzyme: geranyldiphosphate synthase (EC 2.5.1.1)/myrcene synthase (EC 4.2.3.15). When the recombinant enzyme is incubated with dimethylallyl diphosphate and isopentenyl diphosphate as cosubstrates, the level of geranyl diphosphate formed is approximately tenfold higher than the level of myrcene formed. The data suggest that geranyl diphosphate is a free intermediate in myrcene production
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geranyl diphosphate
myrcene + diphosphate
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geranyl diphosphate
myrcene + diphosphate
additional information
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terpenes produced from the wild-type and mutant enzymes are identical, showing low levels of beta-myrcene from AcTPS2-K514A
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geranyl diphosphate
myrcene + diphosphate
O24474
induced by stem wounding, enhances resistance to insects
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geranyl diphosphate
myrcene + diphosphate
Q9ZUH4
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geranyl diphosphate
myrcene + diphosphate
Q58GE8
the acyclic monoterpene myrcene is required for the production of the major aggregation pheromone component, ipsdienol
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geranyl diphosphate
myrcene + diphosphate
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K+
required, optimum concentration of 0.5 M
additional information
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wild-type AcTPS2 is significantly more active when KCl is absent from the activity buffer
Mg2+
ineffective
Mg2+
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10 mM are included in farnesyl diphosphate activity assay
Mg2+
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sequence contains conserved sequences R28(R)X8W and D321DXXD encoing putative Mg2+-binding sites
Mg2+
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required for activity
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7.5
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farnesyl diphosphate activity assay
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farnesyl diphosphate and geranyl diphosphate activity assay
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brenda
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expression correlates well with floral volatile organic compounds emission in scented Alstroemeria genotypes
brenda
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young
brenda
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brenda
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63000
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x * 63000, SDS-PAGE, x * 65300, calculated
65300
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x * 63000, SDS-PAGE, x * 65300, calculated
69278
x * 69278, calculated
72500
calculated MW of recombinant protein expressed in Escherichia coli
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x * 63000, SDS-PAGE, x * 65300, calculated
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recombinant AcTPS2 protein is extracted and purified
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expressed in Escherichia coli
expression in Escherichia coli
mutated enzymes are generated using the QuickChange II site-directed mutagenesis kit. The PCR-based mutagenesis protocol is performed using pET200-D-TOPO harbouring the respective AcTPS2 cDNAs as template. The single-site mutant enzymes overexpressed in Escherichia coli.
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expression in Escherichia coli
expression in Escherichia coli
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expression in Escherichia coli
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expression correlates well with floral volatile organic compounds emission in scented Alstroemeria genotypes
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K514A
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AcTPS2 mutant, 90% reduction in monoterpene synthase activity compared to wild-type enzyme
K514S
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AcTPS2 mutant, presence of Ser is not able to rescue any of the activity lost after the removal of the Lys residue
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MYRS2_ARATH
598
69812
Swiss-Prot
MYRS_OCIBA
599
69963
Swiss-Prot
MYRS_QUEIL
597
69660
Swiss-Prot
TPS2_ANTMA
581
66705
Swiss-Prot
TPS3_ANTMA
584
67339
Swiss-Prot
TPSA_ARATH
591
69279
Swiss-Prot
TPSD2_ABIGR
627
72479
Swiss-Prot
TPS3_SOLLC
607
70652
Swiss-Prot
MTS2_HUMLU
613
72214
Swiss-Prot
A0A0B2SLK6_GLYSO
119
13796
TrEMBL
A0A2G9GQ31_9LAMI
645
74958
TrEMBL
Q58GE8_IPSPI
416
48324
TrEMBL
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Bohlmann, J.; Steele C.L.; Croteau, R.
Monoterpene synthases from grand fir (Abies grandis): cDNA isolation, characterization, and functional expression of myrcene synthase, (-)-(4S)-limonene synthase, and (-)-(1S,5S)-pinene synthase
J. Biol. Chem.
272
21784-21792
1997
Abies grandis, Abies grandis (O24474)
brenda
Bohlmann, J.; Martin, D.; Oldham, N.J.; Gershenzon, J.
Terpenoid secondary metabolism in Arabidopsis thaliana: cDNA cloning, characterization, and functional expression of a myrcene/(E)-beta-ocimene synthase
Arch. Biochem. Biophys.
375
261-269
2000
Arabidopsis thaliana, Arabidopsis thaliana (Q9ZUH4)
brenda
Wise, M.L.; Rorrer, G.L.; Polzin, J.J.; Croteau, R.
Biosynthesis of marine natural products: isolation and characterization of a myrcene synthase from cultured tissues of the marine red alga Ochtodes secundiramea
Arch. Biochem. Biophys.
400
125-132
2002
Ochtodes secundiramea
brenda
Hosoi, M.; Ito, M.; Yagura, T.; Adams, R.P.; Honda, G.
cDNA isolation and functional expression of myrcene synthase from Perilla frutescens
Biol. Pharm. Bull.
27
1979-1985
2004
Perilla frutescens
brenda
Green, S.; Squire, C.J.; Nieuwenhuizen, N.J.; Baker, E.N.; Laing, W.
Defining the potassium binding region in an apple terpene synthase
J. Biol. Chem.
284
8661-8669
2009
Actinidia chinensis
brenda
Gilg, A.B.; Tittiger, C.; Blomquist, G.J.
Unique animal prenyltransferase with monoterpene synthase activity
Naturwissenschaften
96
731-735
2009
Ips pini (Q58GE8)
brenda
Aros, D.; Gonzalez, V.; Allemann, R.K.; Mueller, C.T.; Rosati, C.; Rogers, H.J.
Volatile emissions of scented Alstroemeria genotypes are dominated by terpenes, and a myrcene synthase gene is highly expressed in scented Alstroemeria flowers
J. Exp. Bot.
63
2739-2752
2012
Alstroemeria caryophyllaea
brenda
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