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EC Tree
IUBMB Comments Requires Mg2+. Isolated from the bacterium Streptomyces sp. SANK 60404. This bifunctional enzyme also produces (R)-nephthenol. See EC 4.2.3.149, nephthenol synthase.
The expected taxonomic range for this enzyme is: unclassified Streptomyces
Synonyms
DtcycA ,
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DtcycA
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geranylgeranyl diphosphate = cembrene C + diphosphate
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geranylgeranyl-diphosphate diphosphate-lyase (cembrene-C-forming)
Requires Mg2+. Isolated from the bacterium Streptomyces sp. SANK 60404. This bifunctional enzyme also produces (R)-nephthenol. See EC 4.2.3.149, nephthenol synthase.
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geranylgeranyl diphosphate
cembrene C + diphosphate
geranylgeranyl diphosphate
cembrene C + diphosphate
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cembrene C i.e. (1E,5E,9E)-1,5,9-trimethyl-12-(propan-2-ylidene)cyclotetradeca-1,5,9-triene
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geranylgeranyl diphosphate
cembrene C + diphosphate
the bifunctional enzyme also produces (R)-nephthenol. See EC 4.2.3.149, nephthenol synthase
cembrene C i.e. (1E,5E,9E)-1,5,9-trimethyl-12-(propan-2-ylidene)cyclotetradeca-1,5,9-triene
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?
geranylgeranyl diphosphate
cembrene C + diphosphate
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cembrene C i.e. (1E,5E,9E)-1,5,9-trimethyl-12-(propan-2-ylidene)cyclotetradeca-1,5,9-triene
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?
geranylgeranyl diphosphate
cembrene C + diphosphate
the bifunctional enzyme also produces (R)-nephthenol. See EC 4.2.3.149, nephthenol synthase
cembrene C i.e. (1E,5E,9E)-1,5,9-trimethyl-12-(propan-2-ylidene)cyclotetradeca-1,5,9-triene
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?
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geranylgeranyl diphosphate
cembrene C + diphosphate
geranylgeranyl diphosphate
cembrene C + diphosphate
-
cembrene C i.e. (1E,5E,9E)-1,5,9-trimethyl-12-(propan-2-ylidene)cyclotetradeca-1,5,9-triene
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?
geranylgeranyl diphosphate
cembrene C + diphosphate
-
cembrene C i.e. (1E,5E,9E)-1,5,9-trimethyl-12-(propan-2-ylidene)cyclotetradeca-1,5,9-triene
-
?
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0.0937
geranylgeranyl diphosphate
pH 7.5, 30°C
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0.047
geranylgeranyl diphosphate
pH 7.5, 30°C
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UniProt
brenda
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UniProt
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DTCYA_STRSQ
Streptomyces sp
371
0
41747
Swiss-Prot
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42000
2 * 42000, SDS-PAGE
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homodimer
2 * 42000, SDS-PAGE
homodimer
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2 * 42000, SDS-PAGE
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overexpression in Escherichia coli
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Meguro, A.; Tomita, T.; Nishiyama, M.; Kuzuyama, T.
Identification and characterization of bacterial diterpene cyclases that synthesize the cembrane skeleton
ChemBioChem
14
316-321
2013
Streptomyces sp. (M1V9Q0), Streptomyces sp. SANK 60404 (M1V9Q0)
brenda
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