Information on EC 4.2.3.138 - (+)-epi-alpha-bisabolol synthase

for references in articles please use BRENDA:EC4.2.3.138
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results
Include results (AMENDA + additional results, but less precise)


The expected taxonomic range for this enzyme is: Phyla dulcis

top print hide
EC NUMBER
COMMENTARY hide
4.2.3.138
-
top print hide
RECOMMENDED NAME
GeneOntology No.
(+)-epi-alpha-bisabolol synthase
-
top print hide
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
(2E,6E)-farnesyl diphosphate + H2O = (+)-epi-alpha-bisabolol + diphosphate
show the reaction diagram
-
-
-
-
top print hide
SYSTEMATIC NAME
IUBMB Comments
(2E,6E)-farnesyl-diphosphate diphosphate-lyase (cyclizing, (+)-epi-alpha-bisabolol-forming)
Isolated from the plant Phyla dulcis (Aztec sweet herb). (+)-epi-alpha-Bisabolol is the precursor of the sweetener hernandulcin.
top
top print hide
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
top print hide
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(2E,6E)-farnesyl diphosphate + H2O
(+)-epi-alpha-bisabolol + diphosphate
show the reaction diagram
-
single terpene product in vitro
-
?
top print hide
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0048
(2E,6E)-farnesyl diphosphate
pH 7.4, 30°C; pH 7.5, 30°C
top print hide
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.04
(2E,6E)-farnesyl diphosphate
pH 7.4, 30°C; pH 7.5, 30°C
top print hide
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.4
assay at
top print hide
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
assay at
top print hide
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
top print hide
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
using Ni-NTA chromatography
top print hide
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in yeast and Escherichia coli. The engineered yeast can synthesize a significant amount (0.3 mg per ml) of alpha-bisabolol in shake-flask cultivation; expression in Saccharomyces cerevisiae and Escherichia coli
top
top
top
LINKS TO OTHER DATABASES (specific for EC-Number 4.2.3.138)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
InterPro (database of protein families, domains and functional sites)