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Information on EC 4.2.3.138 - (+)-epi-alpha-bisabolol synthase for references in articles please use BRENDA:EC4.2.3.138Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
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The expected taxonomic range for this enzyme is: Phyla dulcis
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(+)-epi-alpha-bisabolol synthase
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(2E,6E)-farnesyl diphosphate + H2O = (+)-epi-alpha-bisabolol + diphosphate
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(2E,6E)-farnesyl-diphosphate diphosphate-lyase (cyclizing, (+)-epi-alpha-bisabolol-forming)
Isolated from the plant Phyla dulcis (Aztec sweet herb). (+)-epi-alpha-Bisabolol is the precursor of the sweetener hernandulcin.
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(+)-epi-alpha-bisabolol synthase
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UniProt
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(2E,6E)-farnesyl diphosphate + H2O
(+)-epi-alpha-bisabolol + diphosphate
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single terpene product in vitro
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0.0048
(2E,6E)-farnesyl diphosphate
pH 7.4, 30°C; pH 7.5, 30°C
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0.04
(2E,6E)-farnesyl diphosphate
pH 7.4, 30°C; pH 7.5, 30°C
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using Ni-NTA chromatography
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expressed in yeast and Escherichia coli. The engineered yeast can synthesize a significant amount (0.3 mg per ml) of alpha-bisabolol in shake-flask cultivation; expression in Saccharomyces cerevisiae and Escherichia coli
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TPS8_PHYDL
546
63782
Swiss-Prot
A0A2G9GJS1_9LAMI
543
63856
TrEMBL
A0A2G9GJH3_9LAMI
120
14231
TrEMBL
A0A2G9GJJ4_9LAMI
515
60751
TrEMBL
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Attia, M.; Kim, S.U.; Ro, D.K.
Molecular cloning and characterization of (+)-epi-alpha-bisabolol synthase, catalyzing the first step in the biosynthesis of the natural sweetener, hernandulcin, in Lippia dulcis
Arch. Biochem. Biophys.
527
37-44
2012
Phyla dulcis (J7LH11), Phyla dulcis
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