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Information on EC 4.2.3.133 - alpha-copaene synthase and Organism(s) Helianthus annuus and UniProt Accession Q4U3F6

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EC Tree
     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.3 Acting on phosphates
                4.2.3.133 alpha-copaene synthase
IUBMB Comments
Isolated from Helianthus annuus (sunflower). The enzyme also produces beta-caryophyllene, delta-cadinene and traces of other sesquiterpenoids. See EC 4.2.3.13 (+)-delta-cadinene synthase, EC 4.2.3.57 (-)-beta-caryophyllene synthase.
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This record set is specific for:
Helianthus annuus
UNIPROT: Q4U3F6
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The taxonomic range for the selected organisms is: Helianthus annuus
The enzyme appears in selected viruses and cellular organisms
Synonyms
alpha-copaene synthase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
sesquiterpene synthase
-
SYSTEMATIC NAME
IUBMB Comments
(2E,6E)-farnesyl-diphosphate diphosphate-lyase (cyclizing, alpha-copaene-forming)
Isolated from Helianthus annuus (sunflower). The enzyme also produces beta-caryophyllene, delta-cadinene and traces of other sesquiterpenoids. See EC 4.2.3.13 (+)-delta-cadinene synthase, EC 4.2.3.57 (-)-beta-caryophyllene synthase.
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(2E,6E)-farnesyl diphosphate
alpha-copaene + diphosphate
show the reaction diagram
-
delta-cadinene is one of the two main products, while alpha-muuorlene, beta-caryophyllene, alpha-humulene and alpha-copaene are minor products
-
?
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
isoform TPS2; cultivar HA300
UniProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
CS_HELAN
555
0
64263
Swiss-Prot
other Location (Reliability: 4)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
64200
x * 64200, calculated from amino acid sequence
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 64200, calculated from amino acid sequence
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
Ni-NTA affinity column chromatography
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Saccharomyces cerevisiae strain EPY300 as a thioredoxin-fusion protein
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Goepfert, J.C.; Macnevin, G.; Ro, D.K.; Spring, O.
Identification, functional characterization and developmental regulation of sesquiterpene synthases from sunflower capitate glandular trichomes
BMC Plant Biol.
9
86
2009
Helianthus annuus (Q4U3F6)
Manually annotated by BRENDA team