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Information on EC 4.2.3.133 - alpha-copaene synthase and Organism(s) Ricinus communis and UniProt Accession B9S9Z3

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EC Tree
     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.3 Acting on phosphates
                4.2.3.133 alpha-copaene synthase
IUBMB Comments
Isolated from Helianthus annuus (sunflower). The enzyme also produces beta-caryophyllene, delta-cadinene and traces of other sesquiterpenoids. See EC 4.2.3.13 (+)-delta-cadinene synthase, EC 4.2.3.57 (-)-beta-caryophyllene synthase.
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This record set is specific for:
Ricinus communis
UNIPROT: B9S9Z3
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The taxonomic range for the selected organisms is: Ricinus communis
The enzyme appears in selected viruses and cellular organisms
Synonyms
alpha-copaene synthase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
sesquiterpene synthase 1
-
SYSTEMATIC NAME
IUBMB Comments
(2E,6E)-farnesyl-diphosphate diphosphate-lyase (cyclizing, alpha-copaene-forming)
Isolated from Helianthus annuus (sunflower). The enzyme also produces beta-caryophyllene, delta-cadinene and traces of other sesquiterpenoids. See EC 4.2.3.13 (+)-delta-cadinene synthase, EC 4.2.3.57 (-)-beta-caryophyllene synthase.
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(2E,6E)-farnesyl diphosphate
(+)-delta-cadinene + diphosphate
show the reaction diagram
-
yield of 40% (+)-delta-cadinene
-
?
(2E,6E)-farnesyl diphosphate
(-)-alpha-copaene + diphosphate
show the reaction diagram
-
yield of 60% (-)-alpha-copaene, products are about 60% (-)-alpha-copaene, 40% (+)-delta-cadinene
-
?
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
UniProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
TPS1_RICCO
558
0
64392
Swiss-Prot
Chloroplast (Reliability: 4)
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Saccharomyces cerevisiae strain EPY300
exprsssion in Escherichia coli and Saccharomyces cerevisiae
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
synthesis
expression in Saccharomyces cerevisiae leads to a titer of (-)-alpha-copaene of 9 mg/l at 48 h and around 7 mg/l in Escherichia coli, and the titer of (+)-delta-cadinene is 6 mg/l in Saccharomyces cerevisiae and 3.5 mg/l in Escherichia coli
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Xie, X.; Kirby, J.; Keasling, J.D.
Functional characterization of four sesquiterpene synthases from Ricinus communis (castor bean)
Phytochemistry
78
20-28
2012
Ricinus communis (B9S9Z3)
Manually annotated by BRENDA team