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Information on EC 4.2.3.13 - (+)-delta-cadinene synthase and Organism(s) Ricinus communis and UniProt Accession B9S9Z3

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EC Tree
     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.3 Acting on phosphates
                4.2.3.13 (+)-delta-cadinene synthase
IUBMB Comments
The sesquiterpenoid (+)-delta-cadinene is an intermediate in phytoalexin biosynthesis. Mg2+ is required for activity.
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This record set is specific for:
Ricinus communis
UNIPROT: B9S9Z3
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Word Map
The taxonomic range for the selected organisms is: Ricinus communis
The enzyme appears in selected viruses and cellular organisms
Synonyms
sesquiterpene synthase, (+)-delta-cadinene synthase, cdn1-c1, cadinene synthase, delta-cadinene synthase, pntps2, cadinol/cadinene synthase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
2-trans,6-trans-farnesyl-diphosphate diphosphate-lyase (cyclizing, (+)-alpha-cadinene-forming)
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cyclase, delta-cadinene
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delta-cadinene cyclase
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delta-cadinene synthase
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farnesyl diphosphate-delta-cadinene cyclase
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
elimination of diphosphate
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SYSTEMATIC NAME
IUBMB Comments
(2E,6E)-farnesyl diphosphate diphosphate-lyase (cyclizing, (+)-delta-cadinene-forming)
The sesquiterpenoid (+)-delta-cadinene is an intermediate in phytoalexin biosynthesis. Mg2+ is required for activity.
CAS REGISTRY NUMBER
COMMENTARY hide
166800-09-5
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(2E,6E)-farnesyl diphosphate
(+)-delta-cadinene + diphosphate
show the reaction diagram
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products are about 60% (-)-alpha-copaene, 40% (+)-delta-cadinene
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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UniProt
Manually annotated by BRENDA team
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
TPS1_RICCO
558
0
64392
Swiss-Prot
Chloroplast (Reliability: 4)
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
exprsssion in Escherichia coli and Saccharomyces cerevisiae
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
synthesis
expression in Saccharomyces cerevisiae leads to a titer of (-)-alpha-copaene of 9 mg/l at 48 h and around 7 mg/l in Escherichia coli, and the titer of (+)-delta-cadinene is 6 mg/l in Saccharomyces cerevisiae and 3.5 mg/l in Escherichia coli
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Xie, X.; Kirby, J.; Keasling, J.D.
Functional characterization of four sesquiterpene synthases from Ricinus communis (castor bean)
Phytochemistry
78
20-28
2012
Ricinus communis (B9S9Z3)
Manually annotated by BRENDA team