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Information on EC 4.2.3.126 - gamma-muurolene synthase and Organism(s) Santalum album and UniProt Accession B5A435

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EC Tree
     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.3 Acting on phosphates
                4.2.3.126 gamma-muurolene synthase
IUBMB Comments
The enzyme has been characterized from the fungus Coprinus cinereus. Also gives germacrene A and alpha-muurolene, see EC 4.2.3.23, germacrene-A synthase and EC 4.2.3.125, alpha-muurolene synthase.
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This record set is specific for:
Santalum album
UNIPROT: B5A435
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The taxonomic range for the selected organisms is: Santalum album
The enzyme appears in selected viruses and cellular organisms
Synonyms
sasesquitps1, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SasesquiTPS1
-
sesquiterpene synthase
-
SYSTEMATIC NAME
IUBMB Comments
(2E,6E)-farnesyl-diphosphate diphosphate-lase (cyclizing, gamma-muurolene-forming)
The enzyme has been characterized from the fungus Coprinus cinereus. Also gives germacrene A and alpha-muurolene, see EC 4.2.3.23, germacrene-A synthase and EC 4.2.3.125, alpha-muurolene synthase.
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(2E,6E)-farnesyl diphosphate
gamma-muurolene + diphosphate
show the reaction diagram
-
SasesquiTPS1 with (2E,6E)-farnesyl diphosphate as substrate produces a complex mixture of mono- and bicyclic sesquiterpenes. The main products as analysed by GC-MS and GC-FID are the cyclic alcohol germacrene D-4-ol, and helminthogermacrene. Also present are alpha-bulnesene, gamma-muurolene, alpha- and beta-selinenes, as well as several other minor bicyclic compounds, alpha-humulene and nerolidol are detected in proportions below 5%
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mg2+
activates, required
Mn2+
activates, 10fold less affective compared to Mg2+
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
UniProt
Manually annotated by BRENDA team
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
STPS1_SANAL
559
0
65791
Swiss-Prot
-
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant HIs6-tagged SasesquiTPS1 from Escherichia coli by nickel affinity chromatography
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
SasesquiTPS1, DNA and amino acid sequence determination and analysis, sequence comparison, functional expression of His6-tagged full-length enzyme in Escherichia coli
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Jones, C.G.; Keeling, C.I.; Ghisalberti, E.L.; Barbour, E.L.; Plummer, J.A.; Bohlmann, J.
Isolation of cDNAs and functional characterisation of two multi-product terpene synthase enzymes from sandalwood, Santalum album L
Arch. Biochem. Biophys.
477
121-130
2008
Santalum album (B5A435), Santalum album
Manually annotated by BRENDA team