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Information on EC 4.2.3.124 - 2-deoxy-scyllo-inosose synthase and Organism(s) Streptoalloteichus tenebrarius and UniProt Accession Q2MF16

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EC Tree
     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.3 Acting on phosphates
                4.2.3.124 2-deoxy-scyllo-inosose synthase
IUBMB Comments
Requires Co2+ . Involved in the biosynthetic pathways of several clinically important aminocyclitol antibiotics, including kanamycin, butirosin, neomycin and ribostamycin. Requires an NAD+ cofactor, which is transiently reduced during the reaction [1,4]. The enzyme from the bacterium Bacillus circulans forms a complex with the glutamine amidotransferase subunit of pyridoxal 5'-phosphate synthase (EC 4.3.3.6), which appears to stabilize the complex [6,7].
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Streptoalloteichus tenebrarius
UNIPROT: Q2MF16
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Word Map
The taxonomic range for the selected organisms is: Streptoalloteichus tenebrarius
The enzyme appears in selected viruses and cellular organisms
Synonyms
2-deoxy-scyllo-inosose synthase, btrc2, doi synthase, alloh, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
btrC
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-
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kanC
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neoC
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SYSTEMATIC NAME
IUBMB Comments
D-glucose-6-phosphate phosphate-lyase (2-deoxy-scyllo-inosose-forming)
Requires Co2+ [2]. Involved in the biosynthetic pathways of several clinically important aminocyclitol antibiotics, including kanamycin, butirosin, neomycin and ribostamycin. Requires an NAD+ cofactor, which is transiently reduced during the reaction [1,4]. The enzyme from the bacterium Bacillus circulans forms a complex with the glutamine amidotransferase subunit of pyridoxal 5'-phosphate synthase (EC 4.3.3.6), which appears to stabilize the complex [6,7].
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
D-glucose 6-phosphate
2-deoxy-L-scyllo-inosose + phosphate
show the reaction diagram
-
-
-
?
D-glucose 6-phosphate
2-deoxy-L-scyllo-inosose + phosphate
show the reaction diagram
-
-
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
D-glucose 6-phosphate
2-deoxy-L-scyllo-inosose + phosphate
show the reaction diagram
-
-
-
?
D-glucose 6-phosphate
2-deoxy-L-scyllo-inosose + phosphate
show the reaction diagram
-
-
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
2-deoxy-scyllo-inosose (DOI) synthase, which uses glucose-6-phosphate as a substrate for DOI biosynthesis, is indispensably involved in the initial stage of the biosynthesis of 2-deoxystreptamine-containing aminoglycoside antibiotics including butirosin, gentamicin, kanamycin, and tobramycin
metabolism
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DOI synthase is a key enzyme in the biosynthesis of 2-deoxy-scyllo-inosose, DOS
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
gene tbmA, functional recombinant expression codon-optimized gene in Bacillus subtilis strain 168-DOI-15, in which both genes pgi and pgcA are disrupted
gene btrC, DNA and amino acid sequence determination and analysis, sequence comparisons, overexpression in Escherichia coli strain BL21(DE3)
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Kharel, M.; Subba, B.; Lee, H.; Liou, K.; Woo, J.; Kim, D.; Moon, Y.; Sohng, J.
Identification of 2-deoxy-scyllo-inosose synthase in aminoglycoside producer Streptomyces
J. Microbiol. Biotechnol.
13
828-831
2003
no activity in Streptomyces lividans, no activity in Streptomyces lividans TK24, Streptoalloteichus tenebrarius, Streptoalloteichus tenebrarius IFO 13396, Streptomyces kanamyceticus, Streptomyces kanamyceticus IFO13414, Streptomyces ribosidificus, Streptomyces ribosidificus IFO13796
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Manually annotated by BRENDA team
Lim, J.H.; Hwang, H.H.; Lee, N.J.; Lee, J.W.; Seo, E.G.; Son, H.B.; Kim, H.J.; Yoon, Y.J.; Park, J.W.
Enhanced biosynthesis of 2-deoxy-scyllo-inosose in metabolically engineered Bacillus subtilis recombinants
Front. Microbiol.
9
2333
2018
Niallia circulans (Q9S5E2), Streptoalloteichus tenebrarius (Q2MF16), Streptoalloteichus tenebrarius, Streptoalloteichus tenebrarius ATCC 17920 (Q2MF16)
Manually annotated by BRENDA team