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Information on EC 4.2.3.114 - gamma-terpinene synthase and Organism(s) Citrus limon and UniProt Accession Q8L5K4

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EC Tree
     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.3 Acting on phosphates
                4.2.3.114 gamma-terpinene synthase
IUBMB Comments
Isolated from Thymus vulgaris (thyme) [1,2], Citrus limon (lemon) , Citrus unshiu (satsuma) and Origanum vulgare (oregano) . Requires Mg2+. Mn2+ less effective. The reaction involves a 1,2-hydride shift. The 5-pro-S hydrogen of geranyl diphosphate is lost. Traces of several other monoterpenoids are formed in addition to gamma-terpinene.
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This record set is specific for:
Citrus limon
UNIPROT: Q8L5K4
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Word Map
The taxonomic range for the selected organisms is: Citrus limon
The enzyme appears in selected viruses and cellular organisms
Synonyms
gamma-terpinene synthase, tvtps, ovtps2, ostps24, clcts, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ClcTS
-
-
-
-
OvTPS2
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
geranyl-diphosphate diphosphate-lyase (cyclizing, gamma-terpinene-forming)
Isolated from Thymus vulgaris (thyme) [1,2], Citrus limon (lemon) [3], Citrus unshiu (satsuma) [4] and Origanum vulgare (oregano) [5]. Requires Mg2+. Mn2+ less effective. The reaction involves a 1,2-hydride shift. The 5-pro-S hydrogen of geranyl diphosphate is lost. Traces of several other monoterpenoids are formed in addition to gamma-terpinene.
CAS REGISTRY NUMBER
COMMENTARY hide
68943-55-5
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
geranyl diphosphate
gamma-terpinene + diphosphate
show the reaction diagram
-
products are 71.4% gamma-terpinene, with lower amounts of limonene (9.1%), alpha-pinene (5.6%), beta-pinene (4.7%), alpha-terpinolene (3.7%), alpha-thujene (2.5%), alpha-terpinene (1.7%), myrcene (0.9%), sabinene (0.4%) and a trace of p-cymene
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mg2+
or Mn2+, Mn2+ is preferred over Mg2+
Mn2+
or Mg2+, Mn2+ is preferred over Mg2+, optimum concentration 0.6 mM
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0027
geranyl diphosphate
pH 7.0, 30°C
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
UniProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
glants of fruit lavedo and fruit peel
Manually annotated by BRENDA team
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
GTPS_CITLI
600
0
69522
Swiss-Prot
Chloroplast (Reliability: 4)
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Lucker, J.; El Tamer, M.K.; Schwab, W.; Verstappen, F.W.; van der Plas, L.H.; Bouwmeester, H.J.; Verhoeven, H.A.
Monoterpene biosynthesis in lemon (Citrus limon). cDNA isolation and functional analysis of four monoterpene synthases
Eur. J. Biochem.
269
3160-3171
2000
Citrus limon (Q8L5K4)
Manually annotated by BRENDA team