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Information on EC 4.2.3.114 - gamma-terpinene synthase and Organism(s) Origanum vulgare and UniProt Accession E2E2P0

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EC Tree
     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.3 Acting on phosphates
                4.2.3.114 gamma-terpinene synthase
IUBMB Comments
Isolated from Thymus vulgaris (thyme) [1,2], Citrus limon (lemon) , Citrus unshiu (satsuma) and Origanum vulgare (oregano) . Requires Mg2+. Mn2+ less effective. The reaction involves a 1,2-hydride shift. The 5-pro-S hydrogen of geranyl diphosphate is lost. Traces of several other monoterpenoids are formed in addition to gamma-terpinene.
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This record set is specific for:
Origanum vulgare
UNIPROT: E2E2P0
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Word Map
The taxonomic range for the selected organisms is: Origanum vulgare
The enzyme appears in selected viruses and cellular organisms
Synonyms
gamma-terpinene synthase, tvtps, ostps24, ovtps2, clcts, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ClcTS
-
-
-
-
OvTPS2
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
geranyl-diphosphate diphosphate-lyase (cyclizing, gamma-terpinene-forming)
Isolated from Thymus vulgaris (thyme) [1,2], Citrus limon (lemon) [3], Citrus unshiu (satsuma) [4] and Origanum vulgare (oregano) [5]. Requires Mg2+. Mn2+ less effective. The reaction involves a 1,2-hydride shift. The 5-pro-S hydrogen of geranyl diphosphate is lost. Traces of several other monoterpenoids are formed in addition to gamma-terpinene.
CAS REGISTRY NUMBER
COMMENTARY hide
68943-55-5
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
geranyl diphosphate
gamma-terpinene + diphosphate
show the reaction diagram
additional information
?
-
GC-MS analysis of reaction product
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
geranyl diphosphate
gamma-terpinene + diphosphate
show the reaction diagram
-
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mn2+
or Mg2+, required. Mn2+ is preferred over Mg2+, optimum concentration 0.001 mM
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00871
geranyl diphosphate
pH 6.8, 30°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 6.5
half maximal activity
7.2 - 7.5
half maximal activity
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
additional information
GC-MS analysis of plant volatiles at different developmental plant stages and varieties, overview
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
the monoterpene synthase activity is predominantly regulated at the level of transcription, and the phenolic monoterpene alcohol thymol is derived from gamma-terpinene, the product of a single monoterpene synthase. Terpene synthase expression levels directly control the composition of the essential oils. Proportion (%) changes of essential oil components in different varieties of Origanum vulgare, GC-MS analysis of plant volatiles, overview
additional information
tissue-specific expression patterns of genes associated with the terpeniod biosynthesis in two oregano species Origanum vulgare and Origanum majorana, GC-MS analysis of plant volatiles, overview
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
GTPS_ORIVU
594
0
69063
Swiss-Prot
Chloroplast (Reliability: 1)
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
real-time PCR enzyme expression analysis
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
terpene synthase expression levels directly control the composition of the essential oil
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Crocoll, C.; Asbach, J.; Novak, J.; Gershenzon, J.; Degenhardt, J.
Terpene synthases of oregano (Origanum vulgare L.) and their roles in the pathway and regulation of terpene biosynthesis
Plant Mol. Biol.
73
587-603
2010
Origanum vulgare (E2E2P0)
Manually annotated by BRENDA team
Jan, S.; Mir, J.; Shafi, W.; Faktoo, S.; Singh, D.; Wijaya, L.; Alyemeni, M.; Ahmad, P.
Divergence in tissue-specific expression patterns of genes associated with the terpeniod biosynthesis in two oregano species Origanum vulgare L., and Origanum majorana
Indust. Crops Prod.
123
546-555
2018
Origanum majorana, Origanum vulgare (E2E2P0)
-
Manually annotated by BRENDA team